In vivo differentiation and proliferation of mesenchymal stem cells derived from cord blood stem cell transplantation donor.

研究背景：骨髓间充质干细胞在体外培养系统中可分化为骨细胞和软骨细胞。与成人骨髓相比，人脐带血中含有丰富的间充质干细胞。我们分析了来自脐带血干细胞移植供体的间充质干细胞（MSC）在体内分化和增殖的可能性。1)来自供体细胞（脐带血干细胞）的骨和软骨细胞分析一例患有家族性噬血细胞淋巴组织细胞病（FHL）的婴儿接受了脐带血干细胞移植。供体细胞植入后，患者颅骨出现骨溶解，行骨活检。我们采用PCR方法，以20号染色体上的G08062为靶点，分析标本中是否含有供体（脐带血干细胞）来源的间充质干细胞。结果显示与70%的供体来源细胞混合嵌合。然后我们准备了单细胞PCR方法来分析显微解剖法提取的单细胞的来源，但很难从骨组织中获得足够的DNA进行分析。2)供体细胞（脐带血干细胞）骨髓细胞来源分析一例6岁顽固性伯基特淋巴瘤男童接受HLA 2基因不匹配供体的脐带血干细胞移植。我们采用PCR方法，针对18、20、22染色体上的微卫星标记和HLA差异，分析了移植后骨髓不同细胞系嵌合性的变化。不幸的是，移植后不久就发现骨髓复发，因此结果显示100%的受体来源细胞。

Research Background : Mesenchymal stem cells in bone marrow have been known to differentiate into bone and cartilage cells in vitro culture system. Human cord blood is abundant with the mesenchymal stem cells in comparison with adult bone marrow. We analyzed the possibility of differentiation and proliferation of mesenchymal stem cell(MSC) in vivo, which derived from cord blood stem cell transplantation donor.1)Analysis of bone and cartilage cells derived from donor cells (cord blood stem cell)An infant with familial hemophagocytic lymphohistiocytosis(FHL) received a cord blood stem cell transplantation. After the engraftment of the donor cells occurred, osteolysis was found in his skull and bone biopsy was done. We analyzed whether the specimen include the donor (cord blood stem cell) derived MSC or not, using the PCR method targeting the G08062 on the chromosome 20. The results revealed the mixed chimerism with 70% of donor derived cells. Then we prepared the single cell PCR method to analyze the origin of single cell extracted by the microdissection method, but it was difficult to get the enough DNA from the bone tissue for the analyses.2)Analysis of bone marrow cells derived from donor cells (cord blood stem cell)A 6-year-old boy with refractory Burkitt lymphoma received a cord blood stem cell transplantation from HLA 2 loci mismatched donor. We analyzed how the chimerism varied in the different cell lineage of bone marrow after the transplantation, using the PCR method targeting the microsatellite markers on the chromosome 18,20,22 and HLA disparity. Unfortunately, the bone marrow relaplse was found soon after the transplantation, so the results revealed the 100% of recipient derived cells.