In vivo differentiation and proliferation of mesenchymal stem cells derived from cord blood stem cell transplantation donor.

来自脐带血干细胞移植供体的间充质干细胞的体内分化和增殖。

• 批准号: 15591102
• 负责人: KUDO Kazuko
• 金额: $ 1.73万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591102/
• 关键词: cord blood stem cell transplantation; mesenchymal stem cell; chimerism analysis

Research Background : Mesenchymal stem cells in bone marrow have been known to differentiate into bone and cartilage cells in vitro culture system. Human cord blood is abundant with the mesenchymal stem cells in comparison with adult bone marrow. We analyzed the possibility of differentiation and proliferation of mesenchymal stem cell(MSC) in vivo, which derived from cord blood stem cell transplantation donor.1)Analysis of bone and cartilage cells derived from donor cells (cord blood stem cell)An infant with familial hemophagocytic lymphohistiocytosis(FHL) received a cord blood stem cell transplantation. After the engraftment of the donor cells occurred, osteolysis was found in his skull and bone biopsy was done. We analyzed whether the specimen include the donor (cord blood stem cell) derived MSC or not, using the PCR method targeting the G08062 on the chromosome 20. The results revealed the mixed chimerism with 70% of donor derived cells. Then we prepared the single cell PCR method to analyze the origin of single cell extracted by the microdissection method, but it was difficult to get the enough DNA from the bone tissue for the analyses.2)Analysis of bone marrow cells derived from donor cells (cord blood stem cell)A 6-year-old boy with refractory Burkitt lymphoma received a cord blood stem cell transplantation from HLA 2 loci mismatched donor. We analyzed how the chimerism varied in the different cell lineage of bone marrow after the transplantation, using the PCR method targeting the microsatellite markers on the chromosome 18,20,22 and HLA disparity. Unfortunately, the bone marrow relaplse was found soon after the transplantation, so the results revealed the 100% of recipient derived cells.

研究背景：骨髓间充质干细胞在体外培养体系中可分化为骨和软骨细胞。与成人骨髓相比，人脐血中含有丰富的间充质干细胞。分析脐血干细胞移植供体来源的骨髓间充质干细胞(MSC)在体内分化和增殖的可能性。1)供体细胞来源的骨和软骨细胞分析一例家族性噬血细胞淋巴组织细胞增多症(FHL)婴儿接受了脐血干细胞移植。在供体细胞植入后，发现他的头骨有骨溶解，并进行了骨活检。以20号染色体G08062为靶点，用聚合酶链式反应方法分析标本中是否含有供体(脐血干细胞)来源的MSC。结果显示，供体来源的细胞中有70%为混合嵌合体。2)供体细胞(脐血干细胞)来源的骨髓细胞分析1例6岁难治性Burkitt淋巴瘤患儿接受了人类白细胞抗原2(HLA2)不相合供者脐血干细胞移植。以18、20、22号染色体上的微卫星标记和人类白细胞抗原差异为靶点，采用聚合酶链式反应方法分析移植后不同细胞系骨髓嵌合体的变化。不幸的是，移植后不久就发现了骨髓移植，所以结果显示了100%的受者来源细胞。

项目成果

Frequent mutations in the GATA-1 gene in the transient myeloproliferative disorder of Down syndrome

• DOI: 10.1182/blood-2003-02-0390
• 发表时间: 2003-10-15
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Xu, G;Nagano, M;Ito, E
• 通讯作者: Ito, E

Expansion of human CMV-specific cytotoxic T lymphocytes to a clinical scale : a simple culture system using tetrameric HLA-peptide complexes.

将人 CMV 特异性细胞毒性 T 淋巴细胞扩展到临床规模：使用四聚体 HLA-肽复合物的简单培养系统。

• 发表时间: 2004
• 期刊: Cytotherapy. 6
• 作者: Watanabe N;Kojima S;et al.
• 通讯作者: et al.

Induction of Complete Remission of Hypoplastic Leukemia with Antithymocyte Globulin

抗胸腺细胞球蛋白诱导发育不全性白血病完全缓解

• 发表时间: 2003
• 期刊: International journal of hematology
• 影响因子: 2.1
• 作者: A. Yoshimi;Chikako Nakamoto;Yoichi Nakamura;Koji Kato;T. Matsuyama;K. Kudo;S. Kojima
• 通讯作者: S. Kojima

Identification and characterization of polymorphic variations of the ataxia telangiectasia mutated(ATM)

共济失调毛细血管扩张突变体（ATM）多态性变异的鉴定和表征

• 发表时间: 2004
• 期刊: Blood Jan 1;103(1)
• 作者: Takagi M;et al.
• 通讯作者: et al.

Identification and characterization of polymorphic variations of the ataxia telangiectasia mutated (ATM) gene in childhood Hodgkin disease

儿童霍奇金病共济失调毛细血管扩张突变（ATM）基因多态性变异的鉴定和表征

• 发表时间: 2004
• 期刊: Blood 103
• 作者: Takagi M;Mizutani S et al.
• 通讯作者: Mizutani S et al.