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Establishing of cell strain from mouse ES cells for the therapeutic use and study in neurodegenerative disorders

从小鼠 ES 细胞中建立细胞株，用于神经退行性疾病的治疗和研究

基本信息

批准号：
15591125
负责人：
TANAKA Akemi
金额：
$ 2.3万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591125/
关键词：
ES cell glial precursor cell Cell therapy Lysosomal storage disease β-Galactosidase knock-out mouse Neurodegenerative disorder

项目摘要

The study was carried out for treating the brain of neurodegenerative disorders by transplanting cells to deliver the deficit materials.At first, we established ES cell-derived glial precursor cells. ES cells were grown to be embryoid bodies when cultured with insulin, transferrin, and fibronectin, in the absence of LIF. The cells were spread by trypsinization and continued the culture with insulin, transferrin, progesterone, putrescine, and laminin. The cells became bipolar cells when basic fibroblast growth factor (FGF2) and epidermal growth factor (EGF) were added. The cells were stained immunochemically with anti-A2B5, anti-GFAP, and anti-04 antibodies, and confirmed the cell-differentiations.The ES cell-derived glial precursor cells were transplanted into the brain ventricle of β-galactosidase knock-out mouse at 2 weeks of age. One week after the transplantation, the mice were sacrificed and the brain specimens were stained with X-Gal. However, no X-Gal positive cells were found in the brain. When the cells were transplanted in the ventricle of newborn mice, a few X-Gal positive cells were clotted in the brain at one week after the plantation.

这项研究的目的是通过移植细胞来输送缺陷物质来治疗患有神经退行性疾病的大脑。首先，我们建立了ES细胞衍生的神经胶质前体细胞。在缺乏 LIF 的情况下，用胰岛素、转铁蛋白和纤连蛋白培养时，ES 细胞生长为胚状体。通过胰蛋白酶消化将细胞扩散并用胰岛素、转铁蛋白、黄体酮、腐胺和层粘连蛋白继续培养。当添加碱性成纤维细胞生长因子（FGF2）和表皮生长因子（EGF）时，细胞变成双极细胞。用抗A2B5、抗GFAP和抗04抗体对细胞进行免疫化学染色，并证实细胞分化。将ES细胞来源的胶质前体细胞移植到2周龄的β-半乳糖苷酶敲除小鼠的脑室中。移植后一周，处死小鼠，用X-Gal对脑标本进行染色。然而，大脑中没有发现X-Gal阳性细胞。当这些细胞被移植到新生小鼠的心室中时，植入一周后，一些X-Gal阳性细胞在大脑中凝结。

项目成果

期刊论文数量（38）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

ムコ多糖症スクリーニング

粘多糖贮积症筛查

DOI：
发表时间：
2004
期刊：
小児科 45
影响因子：
0
作者：
田中あけみ;坂口知子;戸松俊治;鈴木康之
通讯作者：
鈴木康之

mTOR is essential for growth and proliferation in early mouse embryos and embryonic stem cells

DOI：
10.1128/mcb.24.15.6710-6718.2004
发表时间：
2004-08-01
期刊：
MOLECULAR AND CELLULAR BIOLOGY
影响因子：
5.3
作者：
Murakami, M;Ichisaka, T;Yamanaka, S
通讯作者：
Yamanaka, S

Maeda M, Ohba N, Nakagomi S, Suzuki Y, Kiryu-Seo S, Namikawa K, Kondoh W, Tanaka A, Kiyama H: "Vesicular acetylcholine transporter can be a morphological marker for the reinnervation to muscle of regenerating mortor axons"Neuroscience Res. 48. 305-314 (20

Maeda M、Ohba N、Nakagomi S、Suzuki Y、Kiryu-Seo S、Namikawa K、Kondoh W、Tanaka A、Kiyama H：“囊泡乙酰胆碱转运蛋白可以成为再生运动轴突肌肉神经支配的形态学标记”神经科学研究

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Vesicular acetylcholine transporter can be a morphological marker for the reinnervation to muscle of regenerating mortor axons.

囊泡乙酰胆碱转运蛋白可以作为再生运动轴突肌肉神经支配的形态学标记。

DOI：
发表时间：
2004
期刊：
Neuroscience Research 48
影响因子：
0
作者：
Maeda M;Ohba N;Nakagomi S;Suzuki Y;Kiryu-Seo S;Namikawa K;Kondoh W;Tanaka A;Kiyama H
通讯作者：
Kiyama H

Postischemic administration of Sendai virus vector carrying neurotrophic factor genes prevents delayed neuronal death in gerbils