Association of allergic disorders and FOXP3, the responsible gene for X-linked autoimmunity-allergic disregulation syndrome (XLAAD).

过敏性疾病与 FOXP3 的关联，FOXP3 是 X 连锁自身免疫过敏失调综合征 (XLAAD) 的负责基因。

• 批准号: 15591142
• 负责人: TSUGE Ikuya
• 金额: $ 2.24万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591142/
• 关键词: XLAAD; IPEX; FOXP3; TH1; TH2; Food allergy; anaphylaxis; Foxp3; Th1,Th2サイトカイン; X-linked autoimmunity-allergic disregulation syndrome; アレルギー

1.Allergen-specific helper T-cell response in patients with cow's milk allergyBy using carboxyfluorescein succinimidyl ester (CFSE) dilution assay, both cow's milk protein (CMP)-specific T-cell proliferation and cytokine production were analyzed simultaneously in children with cow's milk allergy (CMA). Significant differences of both CMP-specific CD4+ cell proliferation and TH1/TH2 cytokine production between CMA and non-allergic children were observed. While the proliferative responses of children who recently outgrew CMA were not significantly different from those of patients, the patterns of cytokine production were similar to those of non-allergic children. Proliferation of CD4+IL-10+ regulatory T cell was not different between CMA and non-allergic children.2.An association study between the FOXP3 gene and allergic disorders.A microsatellite polymorphisms, (GT)n, located on intron zero of FOXP3 gene was analysed by ABI PRISM 3100 Genetic Analyzer and an association study between the FOXP3 gene and food allergy were performed. The (GT)_<16> allele showed a significantly lower frequency in patients with food anaphylasxis than in controls, suggesting the possibility that FOXP3 gene may affect severity of food allergy.

1.牛奶过敏患者的过敏原特异性辅助性T细胞反应采用羧基荧光素琥珀酰亚胺酯（CFSE）稀释法，同时检测了牛奶过敏（CMA）患儿的牛奶蛋白（CMP）特异性T细胞增殖和细胞因子产生。CMA与非过敏儿童之间观察到CMP特异性CD 4+细胞增殖和TH 1/TH 2细胞因子产生的显著差异。虽然最近长大的CMA儿童的增殖反应与患者没有显着差异，细胞因子的产生模式与非过敏性儿童相似。采用ABI PRISM 3100型遗传分析仪检测FOXP 3基因第0内含子的微卫星多态性（GT）n，分析FOXP 3基因与食物过敏的相关性。食物过敏患者的（GT）_<16>等位基因频率显著低于对照组，提示FOXP 3基因可能影响食物过敏的严重程度。

项目成果

食物アレルギーの寛解誘導療法

食物过敏的缓解诱导治疗

• 发表时间: 2004
• 期刊: アレルギーの臨床 24巻7号
• 作者: 柘植郁哉;宇理須厚雄
• 通讯作者: 宇理須厚雄

各務美智子, 柘植郁哉等: "IgE結合能に基づいた魚類の関係と生物学的分類"日本小児アレルギー学会誌. 17. 180-189 (2003)

Michiko Kagami、Ikuya Tsuge 等：“基于 IgE 结合能力的鱼类的关系和生物学分类”日本儿科过敏学会杂志 17. 180-189 (2003)。

アレルギー疾患の最新疫学 消化管のアレルギー

过敏性疾病最新流行病学胃肠道过敏

• 发表时间: 2006
• 期刊: アレルギーの臨床 26巻1号
• 作者: 柘植郁哉;宇理須厚雄
• 通讯作者: 宇理須厚雄

柘植郁哉等: "牛乳アレルギー"アレルギー・免疫. 10. 741-744 (2003)

Ikuya Tsuge 等人：“牛奶过敏”过敏与免疫学 10. 741-744 (2003)。

IgE cross-reactivity between fish roes and chicken egg in the patients who have anaphylaxis to salmon roe.

对鲑鱼子过敏的患者，鱼子和鸡蛋之间存在 IgE 交叉反应。

• 发表时间: 2005
• 期刊: Allergology International 7(in press)
• 作者: Y Kondo;M Kakami;I Tsuge et al.
• 通讯作者: I Tsuge et al.