Immunological analysis of the mechanism of oral immunotherapy for food allergy.

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We analyzed the mechanism of oral immunotherapy for food allergy immunologically, with the aim to establish a safe oral immunotherapy. In this study, we evaluated allergen-specific T cells and the activation of basophils in immunotherapy using hypo-allergic hydrolyzed milk. Milk allergen specific T cells were identified as CD154 positive cells after allergen stimulation, and intracytoplasmic cytokines were simultaneously analyzed by multi-color flow-cytometry (Galios). As a result, the milk allergic patients, compared to non-milk allergic patients, milk allergen-specific Th2 cytokine producing cells have increased significantly, and milk allergen-specific IL-4 producing cells were positively correlated with milk specific IgE. On the other hand, in the analysis of basophils, the reduction of Syk involved in signal from Fc epsilon RI was observed after immunotherapy, although the number of cases is still small. It is under investigation by increasing the number of cases currently.