Detect of the new genes which are key regulator of skin growth and differentiation by DNA microarray
通过DNA微阵列检测皮肤生长和分化关键调节因子的新基因
基本信息
- 批准号:15591175
- 负责人:
- 金额:$ 1.92万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The epidermis on the surface of the body is responsible for defense of the body. Many biologists have clarified the mechanisms involved in the growth and differentiation of the skin. The pathways of Ras, Wnt, and Notch signaling, the transcription factors such as Oct family NF-kappaB signaling, retinoid receptors, keratin, catenin, and the integrin families are both important for growth and differentiation of the skin. GPI-anchored proteins are also essential for skin differentiation. Nevertheless, identification of the factors involved in differentiation of the skin remains insufficient, and the search for factors that control the stem cells of the skin has just begun, so that many of these factors remain unknown. We cultured human keratinocytes, promoted their differentiation by adding calcium and extracted the RNA. We then analyzed the genes in order to determine the difference between differentiation and normal condition of the keratinocytes. We found that the expressions of the integrin beta3, RafD, Gp130 transcript variant1, the elastase 3A, IL6 receptor transcript variant 1, and the Ras homolog gene family member 1, PIK3AP1 are reduced when calcium levels are high. These genes are related to Ras signaling and integrin family. On the other hand, we found that the expressions of the hypothetical protein FLJ23168, arrestin 3, the uncharacterized bone marrow protein BM045, TGF bata1 induced transcript 1, cellular retionic acid binding protein 2, the hypothetical protein FLJ14624, and plexin C1 are increased when calcium levels are high.
身体表面的表皮负责身体的防御。许多生物学家已经阐明了皮肤生长和分化的机制。Ras、Wnt和Notch信号传导途径、转录因子如Oct家族NF-κ B信号传导、类维生素A受体、角蛋白、连环蛋白和整联蛋白家族对于皮肤的生长和分化都是重要的。GPI锚定蛋白也是皮肤分化所必需的。然而,对参与皮肤分化的因素的鉴定仍然不足,并且对控制皮肤干细胞的因素的研究刚刚开始,因此这些因素中的许多因素仍然未知。我们培养人角质形成细胞,通过添加钙促进其分化并提取RNA。然后,我们分析了基因,以确定角质形成细胞的分化和正常状态之间的差异。我们发现,当钙水平高时,整合素β 3,RafD,Gp 130转录变体1,弹性蛋白酶3A,IL 6受体转录变体1和Ras同源基因家族成员1,PIK 3AP 1的表达降低。这些基因与Ras信号转导和整合素家族有关。另一方面,我们发现假设蛋白FLJ 23168、抑制蛋白3、未表征的骨髓蛋白BM 045、TGF β 1诱导的转录物1、细胞视黄酸结合蛋白2、假设蛋白FLJ 14624和丛蛋白C1的表达在钙水平高时增加。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Epidermal-specific defect of GPI anchor in Pig-a null mice results in harlequin ichthyosis-like features
- DOI:10.1111/j.0022-202x.2004.23227.x
- 发表时间:2004-09-01
- 期刊:
- 影响因子:6.5
- 作者:Hara-Chikuma, M;Takeda, J;Inoue, S
- 通讯作者:Inoue, S
Inducible activation of Ras and Raf in adult epidermis.
- DOI:
- 发表时间:2003-01
- 期刊:
- 影响因子:11.2
- 作者:M. Tarutani;T. Cai;M. Dajee;P. Khavari
- 通讯作者:M. Tarutani;T. Cai;M. Dajee;P. Khavari
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TARUTANI Masahito其他文献
TARUTANI Masahito的其他文献
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{{ truncateString('TARUTANI Masahito', 18)}}的其他基金
Development of the therapy for psoriasis by using mouse model
利用小鼠模型开发牛皮癣疗法
- 批准号:
21591476 - 财政年份:2009
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of a new therapy and create a mouse model for psoriasis with MAPK signal transmission system
利用MAPK信号传输系统开发银屑病新疗法并创建小鼠模型
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18591247 - 财政年份:2006
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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