BIOLOGICAL CLOCK AND CYTOKINE NETWORK

我们报道了在2004年进行的一项研究中，在UVB照射后12小时，在正常成纤维细胞中观察到b mal-1 mRNA的显著上调和per-1 mRNA的下调。而clock的表达不变。与正常对照相比，来自特应性皮炎患者的成纤维细胞获得了相反的结果。这些结果表明，b mal-1和per-1是分析uvb照射下成纤维细胞生物钟表达模式的有用标记，并且在正常和特应性皮炎中的表达模式与最初预期的不同。今年我们建立了动物模型，分析特应性皮炎患者昼夜颠倒后变应性炎症加重的机制，这是临床上讨论较多的问题。方法将9周龄雄性Balb/c小鼠分为A组和B组，每组又分为实验组和对照组。A组每12小时进行一次UVB照射，连续照射6天。B组与a组同样接受UVB照射，但照射时间较a组偏移12小时。第6天，每只小鼠在侧翼涂0.2% DNFB致敏。第1天对耳廓皮肤进行激射试验。每隔12 h测量耳厚的增加。攻毒试验结束后，每只小鼠接受UVB照射24 h， A组小鼠接受相同照射，B组小鼠在黑暗条件下48 ~ 72 h。“结果”连续UVB照射组与黑暗条件组相比，耳部厚度增加，提示昼夜颠倒影响皮肤炎症。

We reported that significant upregulation of b mal-1 mRNA and downregulation of per-1 were observed at 12 hours after UVB irradiation in normal fibroblasts in the study performed in 2004. While expression of clock was unchanged. Reversed results were obtained in the fibroblasts derived from atopic dermatitis patients when compared to those from normal control. These results suggest that b mal-1 and per-1 are useful markers to analyze expression pattern of biological clock in UVB-irradiated fibroblasts and pattern is different between normal and atopic dermatitis as initially expected. This year we conducted to establish animal model to analyze the mechanism of exacerbation of allergic inflammation after reversal of day and night seen in atopic dermatitis patients which is the matter of discussion in the clinical fields.「Method」Nine week old male Balb/c mice were divided into two A and B groups and each group was subdivided into experimental and control groups. Group A were UVB irradiated for every 12 hours for 6 days. Group B were received same UVB irradiation but 12 hours shift of irradiation schedule from group A. On day 6, each mouse was sensitized with 0.2% DNFB on the flank ski. And on day 1, challenge test was performed on the pinna skin. Increase of ear thickness was measured every 12 hours. Each mouse received UVB irradiation for 24 hours after challenge test and group A received same irradiation and group B were kept under dark condition for next 48-72 hours.「Results」Increased ear thickness was observed in the group which received continuous UVB irradiation in contrast to the group which were kept under dark condition which suggests that reversal of day and night affect skin inflammation.