Mechanism of action of factors involved in regulating the cellular interaction in melanocyte
参与调节黑素细胞细胞相互作用的因子的作用机制
基本信息
- 批准号:15591204
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Mouse epidermal melanoblasts preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in serum-free melanoblast-defined medium (MDM). After 14 days, almost all keratinocytes that existed predominantly in the early stage of primary culture died, and pure cultures of melanoblasts were obtained. Epidermal melanoblasts dramatically increased in number in MDMDF consisting of MDM supplemented with dibutyryl adenosine 3':5'-cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). Epidermal melanocytes increased in number in MDMD consisting of MDM supplemented with DBcAMP. On the other hand, epidermal melanocytes were induced to differentiate in MDMM consisting of MDM supplemented with □-melanocyte-stimulating hormone (MSH). Pure cultured primary melanoblasts or melanocytes in MDMDF or MDMD were further cultured with MDMDF or MDMD supplemented with endothelin (ET)-1 from 14 days. A dramatic increase in the number of melanoblasts … More or melanocytes was observed after 7 days. However, no increase in the number of melanoblasts or melanocytes was observed in the absence of ET-1. The increase in the number of melanoblasts or melanocytes was comparable with that of melanoblasts or melanocytes cocultured with secondary keratinocytes in MDMDF or MDMD. Also, pure cultured primary melanoblasts in MDM were further cultured with MDMM supplemented with ET-1 from 14 days. A dramatic increase in the percentage of melanocytes in the melanoblast-melanocyte population was observed after 7 days. However, no increase in the percentage of melanocytes was observed in the absence of ET-1. The increase was comparable with that of melanocytes cocultured with secondary keratinocytes in MDMM. Moreover, anti- ET-1 antibody inhibited both the proliferation of melanoblasts or melanocytes in MDMDF or MDMD and the differentiation of melanocytes in MDMM in primary culture. These results suggest that ET-1 is one member of the keratinocyte-derived factors that are involved in regulating the proliferation and differentiation of mouse epidermal melanocytes. By using the same method it was shown that ET-2, -3, leukemia inhibitory factor, hepatocyte growth factor, stem cell factor, granulocyte-macrophage colocy-stimulating factor are also one of the keratinocyte-derived factors. Less
小鼠表皮成黑素细胞在无血清成黑素细胞定义培养基(MDM)中优先从新生小鼠皮肤中分离的表皮细胞悬浮液中增殖。14天后,原代培养早期主要存在的角质形成细胞几乎全部死亡,获得纯黑色素细胞培养物。在添加3′:5′-环单磷酸二丁基腺苷(DBcAMP)和碱性成纤维细胞生长因子(bFGF)的MDMDF中,表皮成黑素细胞数量显著增加。在添加DBcAMP的MDMD中,表皮黑色素细胞数量增加。另一方面,在由MDM补充□-黑色素细胞刺激激素(MSH)组成的MDMM中,表皮黑色素细胞被诱导分化。在MDMDF或MDMD中培养的纯原代黑色素母细胞或黑色素细胞在MDMDF或MDMD中添加内皮素(ET)-1培养14天后。7天后观察到更多的黑素细胞。然而,在没有ET-1的情况下,未观察到黑色素母细胞或黑素细胞数量的增加。成黑素细胞或黑素细胞数量的增加与在MDMDF或MDMD中与继发性角质形成细胞共培养的成黑素细胞或黑素细胞数量相当。从第14天开始,用添加ET-1的MDMM进一步培养MDM中纯培养的原代黑素母细胞。7天后,观察到成黑素细胞群中黑素细胞的百分比急剧增加。然而,在没有ET-1的情况下,没有观察到黑素细胞百分比的增加。这种增加与在MDMM中与继发性角质形成细胞共培养的黑色素细胞相当。此外,抗ET-1抗体抑制MDMDF或MDMD中黑色素母细胞或黑色素细胞的增殖以及MDMM中黑色素细胞的分化。这些结果表明ET-1是角化细胞衍生因子之一,参与调节小鼠表皮黑色素细胞的增殖和分化。同样的方法表明,ET-2、-3、白血病抑制因子、肝细胞生长因子、干细胞因子、粒细胞-巨噬细胞结肠刺激因子也是角化细胞衍生因子之一。少
项目成果
期刊论文数量(43)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Role of kerationocyte-derived factors involved in regulating the proliferation and differentiation of mammalian epidermal melanocytes.
角质细胞衍生因子在调节哺乳动物表皮黑素细胞的增殖和分化中的作用。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:T.Hirobe;K.Wakamatsu;S.Ito;et al.;Kusumi I;Mori F at al.;Okamoto Hiroyuki et al.;T.Hirobe.
- 通讯作者:T.Hirobe.
Pheomelanin production in the epidermis from newborn agouti mice is induced by the expression of the agouti gene in the dermis.
新生刺鼠小鼠表皮中褐黑色素的产生是由真皮中刺鼠基因的表达诱导的。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:T.Hirobe;S.Takeuchi;E.Hotta;K.Wakamatsu;S.Ito
- 通讯作者:S.Ito
Pheomelanin production in the epidermis from newborn agouti ice is induced by the expression of the agouti gene in the dermis.
新生刺豚鼠冰在表皮中产生褐黑色素是由真皮中刺豚鼠基因的表达诱导的。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:T.Hirobe;S.Takeuchi;E.Hotta;K.Wakamatsu;S.Ito
- 通讯作者:S.Ito
T.Hirobe, M.Osawa, S-I.Nishikawa.: "Hepatocyte growth factor controls the proliferation of cultured epidermal melanoblasts and melanocytes from newborn mice."Pigment Cell Res.. 17・1. 51-61 (2004)
T.Hirobe、M.Osawa、S-I.Nishikawa.:“肝细胞生长因子控制新生小鼠培养的表皮黑素细胞和黑素细胞的增殖。”Pigment Cell Res.. 17・1 (2004)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
The melanocortin receptor-1 gene but not the proopiomelanocortin gene is expressed in melanoblasts and contributes their differentiation in the mouse skin
- DOI:10.1111/j.1600-0749.2004.00179.x
- 发表时间:2004-12-01
- 期刊:
- 影响因子:0
- 作者:Hirobe, T;Takeuchi, S;Hotta, E
- 通讯作者:Hotta, E
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$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)