Interaction between Iopamidol and Gadopentetate Dimeglumine : An in-vitro experimental study of direct mixing

目的：采用直接混合的方法，在试管中测定碘帕美与Gd-DTPA是否存在相互作用。材料与方法：将iopamidol （Iopamiron 300）与Gd-DTPA （Magnevist）按1:1的比例混合制备试验溶液。立即评估混合溶液的颜色、粘度和pH值，并在混合后1、3、6和24小时评估。测定混合溶液中芳香伯胺浓度、碘帕美醇含量、游离碘离子浓度、Gd-DTPA含量，并采用薄层色谱法测定除碘帕美醇和Gd-DTPA引起的斑点外是否存在斑点。这些试验在混合后立即和24小时进行。以单用iopamidol和单用Gd-DTPA作为对照，检查相同的项目。结果：与Gd-DTPA溶液混合后即刻及直接混合后1.3、6、24 h外观及pH均无明显变化。单用碘帕美多尔和单用Gd-DTPA的研究未见明显异常值或发现。结论：吡帕美与Gd-DTPA在试管内直接混合使用无明显相互作用。

Objective : To determine whether or not there is any interaction between iopamidol and Gd-DTPA in test tubes using the direct mixing manner.Materials and Methods : The test solution was prepared by mixing iopamidol (Iopamiron 300) and Gd-DTPA (Magnevist) at a ratio of 1:1. The color, viscosity, and pH of the mixed solutions were assessed immediately and 1,3,6,and 24 hours after mixing. The concentration of aromatic primary amines, content of iopamidol, concentration of free iodine ion, and content of Gd-DTPA in the mixed solution were determined, and presence or absence of spots, other than those resulting from iopamidol and Gd-DTPA, was determined using thin layer chromatography. These tests were carried out immediately and 24 hours after mixing. Using iopamidol alone and Gd-DTPA alone as controls, the same items were examined.Results : There was no significant change in the appearance or pH of the mixed iopamidol and Gd-DTPA solution immediately and 1.3,6,and 24 hours after direct mixing. The study of iopamidol alone and Gd-DTPA alone showed no apparently abnormal values or findings.Conclusion : The direct mixing of iopamidol and Gd-DTPA in a test tube results in no significant interaction.