An involvement and influence of periodontopathic bacteria for aortic atherosclerotic lesions

牙周病菌对主动脉粥样硬化病变的参与及影响

• 批准号: 15591331
• 负责人: INOUE Yoshinori
• 金额: $ 1.92万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591331/
• 关键词: arteriosclerosis obliterans; abdominal aortic aneurysm; periodontitis; periodotopathic bacteria; cytokines; サイトカン; 動脈疾患; 単球中サイトカイン; 動脈瘤; TNF-α; IL-1β

Recent epidemiologic survey reported a relationship between periodontitis and cardiovascular or carotid artery diseases. We investigated a relationship between arterial disease and periodontitis or monocyte cytokines. The patients comprised of arteriosclerosis obliterans (ASO group) : 55 cases, and abdominal aortic aneurysms (AAA group) : 50 cases. The patients were evaluated as grading of periodontal disease, periodontopathic bacteria-DNA of the saliva and arterial lesions by polymerase chain reaction, and localization of periodontopathic bacteria-DNA. The patients also underwent blood analysis and measurement of monocyte cytokines (TNF-α, IL-1β) after LPS stimulation.The grading of periodontitis was severe (dental pocket > 5mm) : 26 cases, moderate (2-5mm) : 19 cases, normal (< 2mm) : 2 cases, and edentulous 8 cases in the ASO group. The grading of periodontitis was severe : 28 cases, moderate : 14 cases, normal : 2 cases, and edentulous : 6 cases in the AAA group. In the ASO group, … More positive rates of periodontopathic bacteria-DNA were 40/53 (94%), 9/10 (90%), and 21/43 (48%), in the saliva, atherosclerotic lesion, and anastomotic site (mild lesion), respectively. In contrast, the positive rates of the AAA group were 43/50 (86%), and 41/50 (82%), in the saliva, and aneurysmal wall, respectively. Positive rate of periodontopathic bacteria-DNA was significantly higher in AAA (86%) than in ASO (56.6%). Localization of periodontopathic bacteria-DNA was evaluated in 10 patients, which was detected at all three layers in all five patients with AAA, and only at the intima and media in all five patients with ASO. Fluorescent intensity of monocyte TNF-α was significantly increased in the AAA group compared with the ASO group.Prevalence of periodontitis was higher than that of Japanese population, and periodontopathic bacteria-DNA was frequently detected at the arterial lesions. Periodontopathic bacteria were supposed to influence arterial disease by identification of periodontopathic bacteria-DNA at the arterial wall. TNF-α upregulated production of matrix metalloproteinase, which degraded collagen of the aortic wall. Fluorescent intensity of monocyte TNF-α was significantly higher in the AAA group than in the ASO group, which was supposed to be related with aneurysmal formation. Less

最近的流行病学调查报告牙周炎与心血管或颈动脉疾病的关系。我们调查了动脉疾病和牙周炎或单核细胞因子之间的关系。其中动脉硬化闭塞症（阿索）55例，腹主动脉瘤（AAA）50例。对患者进行牙周病分级、聚合酶链反应检测唾液和动脉病变中的牙周致病菌DNA、牙周致病菌DNA定位。阿索组重度牙周炎（牙囊> 5 mm）26例，中度牙周炎（2- 5 mm）19例，正常牙周炎（牙囊<2 mm）2例，无牙颌8例。AAA组中重度牙周炎28例，中度牙周炎14例，正常牙周炎2例，缺牙6例。在阿索组中， ...更多信息 唾液、动脉粥样硬化病变和吻合口（轻度病变）中牙周致病菌DNA的阳性率分别为40/53（94%）、9/10（90%）和21/43（48%）。而AAA组唾液和囊壁的阳性率分别为43/50（86%）和41/50（82%）。AAA组牙周致病菌DNA阳性率（86%）明显高于阿索组（56.6%）。在10名患者中进行了牙周病细菌-DNA的定位评估，在所有5名AAA患者的所有三个层中均检测到牙周病细菌-DNA，而在所有5名阿索患者中仅在内膜和中膜中检测到牙周病细菌-DNA。AAA组单核细胞TNF-α荧光强度较阿索组显著增高，牙周炎患病率高于日本人群，动脉病变处经常检测到牙周致病菌DNA。通过对动脉壁牙周致病菌DNA的鉴定，推测牙周致病菌对动脉疾病的影响。TNF-α上调基质金属蛋白酶的产生，从而降解主动脉壁的胶原。AAA组单核细胞TNF-α的荧光强度明显高于阿索组，可能与肿瘤形成有关。少

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