An association between peripheral artery disease and periodontal pathogen relating with platelet aggregation

外周动脉疾病与血小板聚集相关牙周病原菌之间的关系

• 批准号: 22591399
• 负责人: INOUE Yoshinori
• 金额: $ 2.5万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591399/
• 关键词: 歯周病; 血書版凝集能; 血小板凝集能; 動脈疾患; 閉塞性動脈硬化症; P.gingivalis; 全血血小板凝集能; 歯周病菌Ig抗体価

We reported that Porphyromonas gingivalis (P. gingivalis) induced human platelet aggregation. Platelet aggregation using whole blood was assessed between patients with peripheral artery disease and normal volunteers. P. gingivalis induced platelet aggregation increased in PAD patients than normal control, which showed higher in patients with Fontaine III/IV compared with Fontaine II, and exhibited positive correlation with IgG titer for P. gingivalis. FcrRIIa/IIIb(CD32) antibody pretreatment resulted PAD patients in three categories including complete abolishment, partial suppression, and full reaction of Pg induced platelet aggregation. When platelet aggregation was induced sufficiently after CD32 antibody pretreatment (more than 5 ohm), Pg did not induce additionalplatelet aggregation. In contrast, Pg induced platelet aggregation was not suppressed when slight upregulation was shown (less than 1-3 ohm), suggesting that CD32 antibody and Pg induced platelet aggregation competitively. Platelet aggregation was induced after tooth brushing in patients with severe periodontitis. It was suggested that P. gingivalis involved in PAD aggravation by platelet activation.

我们报道了牙龈卟啉单胞菌（P. gingivalis）诱导人血小板聚集。外周动脉疾病患者和正常志愿者全血血小板聚集情况进行了评估。牙龈卟啉单胞菌诱导的血小板聚集在PAD患者中高于正常对照组，且方丹III/IV组高于方丹II组，且与牙龈卟啉单胞菌IgG滴度呈正相关。FcrRIIa/IIIb（CD32）抗体预处理使PAD患者出现Pg诱导的血小板聚集完全消除、部分抑制和完全反应三种情况。当CD32抗体预处理（大于5欧姆）后充分诱导血小板聚集时，Pg不诱导额外的血小板聚集。相比之下，Pg诱导的血小板聚集在轻微上调（小于1-3欧姆）时不受抑制，提示CD32抗体和Pg竞争性地诱导血小板聚集。重度牙周炎患者刷牙后可诱导血小板聚集。提示牙龈卟啉卟啉菌通过血小板活化参与PAD的加重。

项目成果

Outcome of coil embolization for internal iliac artery in patients with endovascular abdominal aortic aneurysm repair

腹主动脉瘤腔内修复术患者髂内动脉弹簧圈栓塞治疗效果

• 发表时间: 2011
• 作者: Uchiyama H;Inoue Y
• 通讯作者: Inoue Y

重症虚血肢に対する下肢血行再建術、持続陰圧吸引療法と遊離筋皮弁術による集学的治療

下肢血运重建、持续负压吸引、游离肌皮瓣手术等多学科治疗严重肢体缺血

• 发表时间: 2012
• 期刊: 日血外会誌
• 作者: 猪狩公宏;田中顕太郎;工藤敏文;豊福崇浩;地引政利;井上芳徳
• 通讯作者: 井上芳徳

Conservative treatment for isolated superior mesenteric artery dissection

• DOI: 10.1007/s00595-012-0304-8
• 发表时间: 2013-03-01
• 期刊: SURGERY TODAY
• 影响因子: 2.5
• 作者: Jibiki, Masatoshi;Inoue, Yoshinori;Kudo, Toshifumi
• 通讯作者: Kudo, Toshifumi

Multidisciplinary Approach to a Peripheral Arteriovenous Malformation

外周动静脉畸形的多学科治疗方法

• 发表时间: 2012
• 期刊: EJVES Extra
• 作者: Igari K;Kudo T;Toyofuku T;Jibiki M;Inoue Y
• 通讯作者: Inoue Y

The usefulness of the post-exercise common femoral artery doppler waveform and peak systolic velocity

运动后股总动脉多普勒波形和收缩期峰值速度的有用性

• 发表时间: 2011
• 作者: Nakashima R;Miyai M;Kagayama T;Toyofuku T;Kudo T;Jibiki M;Inoue Y;Usui A
• 通讯作者: Usui A