Amelioration of engraftment after islet transplantation

背景：胰岛植入的不一致性仍然是胰岛移植应用的主要障碍。本研究旨在探讨中性粒细胞弹性蛋白酶抑制剂在胰岛移植中对受损胰岛的保护作用。将胰岛移植于受体大鼠门静脉。分别于移植前及移植后24小时测定肝脏胰岛素含量。结果：移植后24小时，移植胰岛存活率为32.4%。中性粒细胞弹性蛋白酶抑制剂的管理并没有改善胰岛的存活率。结论：神经弹性蛋白酶抑制剂在胰岛移植中不起作用。说明中性粒细胞在移植胰岛中的浸润在胰岛植入过程中并不起重要作用。另一机制有待进一步研究以改善移植胰岛植入率低的问题。

Background :The inconsistency of islet engraftment remains the major obstacle to the application of islet transplantation. This study addresses the impact of neutrophil elastase inhibitor applied during islet transplantation to rescue the injured islets.Method :Islets from Wistar rat were purified in an usual manner. Islets were transplanted into portal vein in recipient rats. The insulin content in the liver were measured before and 24hr after transplantation. The ratio of insulin content in liver was regarded as the islet-survival rate (%) after transplantation.Results :The survival rate of transplanted islets was 32.4% at 24hr after transplantation. The administration of neutrophol elastase inhibitor did not ameliorate the survival rate of islets. The rate was 34.3%.Conclusions :Neutrophil elastase inhibitor did not work in the islet transplantation. It means that the infiltration of neutrophil in the transplanted islets did not play an important role in islet engraftment. Another mechanism should be researched to improve the low engraftment of transplanted islets.