Amelioration of engraftment after islet transplantation

胰岛移植后植入的改善

• 批准号: 15591374
• 负责人: SHIBATA Satoshi
• 金额: $ 1.98万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591374/
• 关键词: neutrophl elastase inhibitor; islet transplantation; engraftment; 好中球浸潤; 虚血再灌流傷害; グラフト生着率

Background :The inconsistency of islet engraftment remains the major obstacle to the application of islet transplantation. This study addresses the impact of neutrophil elastase inhibitor applied during islet transplantation to rescue the injured islets.Method :Islets from Wistar rat were purified in an usual manner. Islets were transplanted into portal vein in recipient rats. The insulin content in the liver were measured before and 24hr after transplantation. The ratio of insulin content in liver was regarded as the islet-survival rate (%) after transplantation.Results :The survival rate of transplanted islets was 32.4% at 24hr after transplantation. The administration of neutrophol elastase inhibitor did not ameliorate the survival rate of islets. The rate was 34.3%.Conclusions :Neutrophil elastase inhibitor did not work in the islet transplantation. It means that the infiltration of neutrophil in the transplanted islets did not play an important role in islet engraftment. Another mechanism should be researched to improve the low engraftment of transplanted islets.

背景：胰岛植入的不一致性一直是胰岛移植应用的主要障碍。本研究旨在探讨中性粒细胞弹性蛋白酶抑制剂在胰岛移植中的应用对损伤胰岛修复的影响。将胰岛移植到受体大鼠的门静脉。分别于移植前和移植后24小时测定肝脏胰岛素含量。结果：移植后24小时胰岛存活率为32.4%。给予中性粒细胞弹性酶抑制剂并不能改善胰岛的存活率。总有效率为34.3%。结论：中性粒细胞弹性蛋白酶抑制剂在胰岛移植中无效。说明中性粒细胞在移植胰岛内的渗透在胰岛植入过程中不起重要作用。另一种机制需要研究，以改善移植胰岛的低植入率。

项目成果

Outcomes and indications of segmental pancreatectomy. Comparison with distal pancreatectomy.

节段性胰腺切除术的结果和适应症。

• 发表时间: 2004
• 期刊: Dig Surg 21・1
• 作者: Shibata S.;et al.
• 通讯作者: et al.

Reversal of diabetes in non-immunosuppressed rhesus macaques by intraportal porcine islet xenografts precedes acute cellular rejection

• DOI: 10.1111/j.1399-3089.2004.00157.x
• 发表时间: 2004-09-01
• 期刊: XENOTRANSPLANTATION
• 影响因子: 3.9
• 作者: Kirchhof, N;Shibata, S;Hering, BJ
• 通讯作者: Hering, BJ