Analysis of Sema4D/ ARHGEF10 signal transduction on the myelin seath formation of Schwann cells

虽然ARHGEF10（鸟嘌呤交换因子）被称为Rho家族蛋白GEF，与肌动蛋白细胞骨架的调节有关，但ARHGEF10的功能尚不清楚。由于在开始对ARHGEF10在雪旺细胞中的功能分析之前，需要获得ARHGEF10的基本功能信息，因此我们对ARHGEF10和ARHGEF10耗尽细胞的细胞质定位进行了研究。这些研究表明，ARHGEF10定位于从高尔基体转运到质膜的囊泡，其功能与胞外化囊泡的胞质转运有关。

Though ARHGEF10 (guanine exchange factor) is known as GEF for Rho family protein which is related to the regulation of actin cytoskeleton, the function of ARHGEF10 had been unknown. Because it was necessary to obtain the basal functional information of ARHGEF10 before starting the functional analysis of ARHGEF10 in the Schwann cells, we carried out the research about the cytoplasmic localization of ARHGEF10 and ARHGEF10 depleted cells. These research indicated that ARHGEF10 localized at the vesicles which is transported from golgi-apparatus to plasmamembrane and the function of ARHGEF10 is related to the cytoplasmic transport of the exocytosed vesicles.