Characterization of clinically useful human monoclonal antibodies against cancers of digestive organ origin.

目的：SKI是一种人类免疫球蛋白单抗，可识别AgSK1抗原，该抗原在人类腺癌，特别是胃肠道恶性肿瘤中优先表达。本研究旨在探讨结直肠癌组织中AgSK1基因表达的临床病理意义。方法：采用定量RT-PCR方法检测40例结直肠癌手术切除标本中AgSK1基因的表达水平。计算肿瘤组织值(T值)、非肿瘤组织值(N值)、T值与N值的比值(T/N值)。为了进一步分析，我们将这些数据与临床病理特征进行了比较。结果：在有淋巴结转移状态中，PN阳性组AgSK1mRNA的T值明显高于PN阴性组(P=0.076)。根据平均T值(8.032)或平均T/N比(1.549)，我们将这些患者分为两组，低表达组和高表达组。高表达组阳性淋巴结转移率明显高于对照组(T值：P=0.021，T/N值；P=0.024)。结论：肿瘤组织中AgSK1mRNA的表达可能成为判断结直肠癌淋巴结转移的有用指标和潜在的恶性标志物。

PURPOSE : SKI, a human IgM monoclonal antibody recognizes the antigen, termed AgSK1 which was shown to be preferentially expressed by human adenocarcinomas, particularly human gastrointestinal malignancies. The aim of this study was to clarify the clinicopathological significance of AgSK1 mRNA expression in human colorectal cancer. METHODS : Using a quantitative RT-PCR, we studied the mRNA expression level of AgSK1 in the resected specimen of 40 patients with colorectal cancer. We estimated the tumor tissue value (T-value), nontumorous tissue value (N-value), and the ratio of T-value to N-value (T/N Ratio). For further analysis, we compared these data with the clinicopathological features. RESULTS : In the status of lymph node metastases, pN positive status tended to have a higher T-value level of AgSK1 mRNA than pN negative status (P=0.076). According to a mean T-value (8.032) or a mean T/N Ratio (1.549), we divided these patients into two groups, low expression group and high expression group. A high expression group showed a significantly higher frequency of positive lymph node metastases (T-value ; P=0.021,T/N Ratio ; P=0.024). CONCLUSION : AgSK1 mRNA expression in tumor tissue may become a useful marker for lymph node metastases and a malignant potential marker of colorectal cancer.