Characterization of clinically useful human monoclonal antibodies against cancers of digestive organ origin.

临床上有用的抗消化器官来源的癌症的人单克隆抗体的表征。

• 批准号: 15591376
• 负责人: KODA Keiji
• 金额: $ 1.34万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591376/
• 关键词: human monoclonal antibody; gastrointestinal malignancy; SK-1; Colon cancer; lymph node metastasis; 乳癌; 特異的癌免疫治療

PURPOSE : SKI, a human IgM monoclonal antibody recognizes the antigen, termed AgSK1 which was shown to be preferentially expressed by human adenocarcinomas, particularly human gastrointestinal malignancies. The aim of this study was to clarify the clinicopathological significance of AgSK1 mRNA expression in human colorectal cancer. METHODS : Using a quantitative RT-PCR, we studied the mRNA expression level of AgSK1 in the resected specimen of 40 patients with colorectal cancer. We estimated the tumor tissue value (T-value), nontumorous tissue value (N-value), and the ratio of T-value to N-value (T/N Ratio). For further analysis, we compared these data with the clinicopathological features. RESULTS : In the status of lymph node metastases, pN positive status tended to have a higher T-value level of AgSK1 mRNA than pN negative status (P=0.076). According to a mean T-value (8.032) or a mean T/N Ratio (1.549), we divided these patients into two groups, low expression group and high expression group. A high expression group showed a significantly higher frequency of positive lymph node metastases (T-value ; P=0.021,T/N Ratio ; P=0.024). CONCLUSION : AgSK1 mRNA expression in tumor tissue may become a useful marker for lymph node metastases and a malignant potential marker of colorectal cancer.

目的：SKI 是一种人类 IgM 单克隆抗体，可识别称为 AgSK1 的抗原，该抗原被证明优先由人类腺癌，特别是人类胃肠道恶性肿瘤表达。本研究的目的是阐明AgSK1 mRNA表达在人结直肠癌中的临床病理意义。方法：采用定量RT-PCR方法，研究了40例结直肠癌患者的切除标本中AgSK1 mRNA的表达水平。我们估计了肿瘤组织值（T 值）、非肿瘤组织值（N 值）以及 T 值与 N 值之比（T/N 比）。为了进一步分析，我们将这些数据与临床病理特征进行了比较。结果：在淋巴结转移状态中，pN阳性状态的AgSK1 mRNA T值水平往往高于pN阴性状态（P=0.076）。根据平均T值（8.032）或平均T/N比（1.549），我们将这些患者分为两组，低表达组和高表达组。高表达组显示阳性淋巴结转移的频率显着较高（T值；P=0.021，T/N比；P=0.024）。结论:肿瘤组织中AgSK1 mRNA的表达可能成为淋巴结转移的有用标志物和结直肠癌的潜在恶性标志物。

项目成果

Natural killer cell activity and distant metastasis in rectal cancers treated surgically with/without neoadjuvant cehmo-radiotherapy.

使用/不使用新辅助化疗的手术治疗直肠癌的自然杀伤细胞活性和远处转移。

• 发表时间: 2003
• 期刊: J Am Col Surg 197
• 作者: Keiji Koda;et al.
• 通讯作者: et al.

Kondo E, Koda K, et al.: "Preoperative natural killer cell activity as a prognostic factor for distant metastasis following surgery for advanced colon cancer"Digestive Surg. 20. 445-451 (2003)

Kondo E、Koda K 等人：“术前自然杀伤细胞活性作为晚期结肠癌手术后远处转移的预后因素”消化外科。

Keiji Koda, et al.: "Natural killer cell activity and distant metastasis in rectal cancers treated surgically with/without neoadjuvant chemo-radiotherapy"J Am Col Surg. 197. 254-260 (2003)

Keiji Koda 等人：“使用/不使用新辅助化疗放疗进行手术治疗的直肠癌中的自然杀伤细胞活性和远处转移”J Am Col Surg。

Preoperative natural killer cell activity as a prognostic factor for distant metastasis following surgery for colon cancer

• DOI: 10.1159/000072714
• 发表时间: 2003-01-01
• 期刊: DIGESTIVE SURGERY
• 影响因子: 2.7
• 作者: Kondo, E;Koda, K;Miyazaki, M
• 通讯作者: Miyazaki, M

Oda K, Koda K, et al.: "Detection of Epstein-Barr virus in gastric carcinoma cells and surrounding lymphocytes"Gastric Cancer. 6. 173-178 (2003)

Oda K、Koda K 等人：“胃癌细胞和周围淋巴细胞中 Epstein-Barr 病毒的检测”胃癌。