The functional analysis of Helicobacter pylori VacA toxin on the proliferation of remnant stomach cancer

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Recent studies show that transepithelial ion transport plays an important role in fundamental cellular functions such as cell proliferation and cell death. Particularly, chloride ion transport is reported to affect cell proliferation by regulating cell volume. In addition, mitochondria which have an extremely important role as energy-producing organella are known to be influenced by intracellular ion environments. In the present study, we studied whether chloride ion transport regulates cell proliferation and activity of mitochondria in renal epithelial A6 cells by using regulators of chloride ion channel and Na+/K+/2Cl-cotransporter (NKCC). Treatment with NPPB (a chloride ion channel blocker) and quercetin (an NKCC regulator) inhibited cell growth and activity of mitochondria, but bumetanide (an NKCC inhibitor) did not affect them. We have reported that regulators of chloride ion transport would influence cell proliferation and mitochondrial activity by regulating intracellular ion environments. Based on these results, we have started the same assay of human gastric cancer cell lines instead of frog renal epithelial cells. And also we have been extracting Helicobacter pylori VacA toxin to evaluate the function of the toxin in this assay system. On the other hand, we have reported the clinico-pathological and genetic analysis concerning human gastric cancer, especially remnant stomach cancer as references comprehensively. These research projects will bring the great advancement in human remnant stomach cancer.Supported by JSPS 15659053 (YM), 15590189 (NN), 15790120 (HM)