The functional analysis of Helicobacter pylori VacA toxin on the proliferation of remnant stomach cancer

幽门螺杆菌VacA毒素对残胃癌增殖的功能分析

• 批准号: 15591427
• 负责人: ITOI Hirosumi
• 金额: $ 1.86万
• 依托单位: Meiji University of Oriental Medicine(M.U.O.M), Graduate School of Oriental Medicine (2004)Kyoto Prefectural University of Medicine (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591427/
• 关键词: Helicobacter pylori; Vac-A toxin; CagA gene; Cloride channel; human gastric cancer; remnant stomach cancer

Recent studies show that transepithelial ion transport plays an important role in fundamental cellular functions such as cell proliferation and cell death. Particularly, chloride ion transport is reported to affect cell proliferation by regulating cell volume. In addition, mitochondria which have an extremely important role as energy-producing organella are known to be influenced by intracellular ion environments. In the present study, we studied whether chloride ion transport regulates cell proliferation and activity of mitochondria in renal epithelial A6 cells by using regulators of chloride ion channel and Na+/K+/2Cl-cotransporter (NKCC). Treatment with NPPB (a chloride ion channel blocker) and quercetin (an NKCC regulator) inhibited cell growth and activity of mitochondria, but bumetanide (an NKCC inhibitor) did not affect them. We have reported that regulators of chloride ion transport would influence cell proliferation and mitochondrial activity by regulating intracellular ion environments. Based on these results, we have started the same assay of human gastric cancer cell lines instead of frog renal epithelial cells. And also we have been extracting Helicobacter pylori VacA toxin to evaluate the function of the toxin in this assay system. On the other hand, we have reported the clinico-pathological and genetic analysis concerning human gastric cancer, especially remnant stomach cancer as references comprehensively. These research projects will bring the great advancement in human remnant stomach cancer.Supported by JSPS 15659053 (YM), 15590189 (NN), 15790120 (HM)

最近的研究表明，跨上皮离子转运在细胞增殖和细胞死亡等基本细胞功能中起着重要作用。特别地，据报道氯离子转运通过调节细胞体积来影响细胞增殖。此外，已知作为产生能量的细胞器具有极其重要作用的线粒体受细胞内离子环境的影响。本研究应用氯离子通道调节剂和Na+/K+/2Cl-协同转运蛋白（Na+/K+/2Cl-cotransporter，NKCC）研究了氯离子转运对肾上皮A6细胞增殖和线粒体活性的调节作用。用NPPB（一种氯离子通道阻断剂）和槲皮素（一种NKCC调节剂）处理抑制细胞生长和线粒体活性，但布美他尼（一种NKCC抑制剂）不影响它们。我们已经报道了氯离子转运的调节剂可以通过调节细胞内离子环境来影响细胞增殖和线粒体活性。基于这些结果，我们开始了相同的人胃癌细胞系而不是青蛙肾上皮细胞的测定。同时，我们还对幽门螺杆菌VacA毒素进行了提取，并对该毒素在该检测系统中的作用进行了评价。另一方面，我们对人类胃癌，特别是残胃癌的临床病理和遗传学分析作了全面的报道。这些研究项目将为人类残胃癌的研究带来巨大进步。JSPS 15659053（YM）、15590189（NN）、15790120（HM）支持

项目成果

S-1-induced, prolonged complete of lung metastasis from gastric cancer refractory to 5'-DFUR ; a case report with pharmacokinetic study.

S-1诱导的、5-DFUR难治性胃癌肺转移的延长完成；

• 发表时间: 2004
• 期刊: Jpn J Clin Oncol. 34巻5号
• 作者: Ueda Y;Yamagishi H;Itoi H et ai.
• 通讯作者: Itoi H et ai.

萩原明於, 糸井啓純, 山岸久一他: "食道浸潤胃癌に対する横隔膜脚合併切除による新しいリンパ節完全郭清手技"京都府立医科大学雑誌. 第112巻・12号. 887-889 (2003)

Akio Hagiwara、Keizumi Itoi、Hisakazu Yamagishi 等：“一种新的完整淋巴结清扫技术，联合膈腿切除术治疗侵袭食管的胃癌”，京都府立医科大学学报，第 112 卷，第 12 期。887。 -889 (2003)

The extending range of the tumor is a more suitable predictive risk factor for lymph node metastases than the location of the deepest tumor invasion in distal thoracic esophageal and cardiac cancer

在远端胸段食管癌和贲门癌中，肿瘤的延伸范围是比肿瘤最深浸润位置更适合的淋巴结转移预测危险因素

• 发表时间: 2005
• 期刊: Oncol Rep. (in press)
• 作者: Shiozaki A;Yamagishi H;Itoi H et al.
• 通讯作者: Itoi H et al.

充実型および非充実型胃低分化腺癌の臨床病理学的検討

实性和非实性胃低分化腺癌的临床病理学检查

• 发表时间: 2003
• 期刊: 京都府立医科大学雑誌 第112巻・12号
• 作者: 大辻英吾;糸井啓純;山岸久一他
• 通讯作者: 山岸久一他

S-1-induced, prolonged complete regression of lung metastasis from gastric cancer refractory to 5'-DFUR : a case report with pharmacokinetic study.

S-1 诱导的 5-DFUR 难治性胃癌肺转移的长期完全消退：药代动力学研究病例报告。

• 发表时间: 2004
• 期刊: Jpn J Clin Oncol. 34巻・5号
• 作者: Ueda Y;Yamagishi H;Itoi H etc
• 通讯作者: Itoi H etc