The establishment of liver metastasis model in colonic cancer and its gene exression using microarray analyses

结肠癌肝转移模型的建立及其基因表达的微阵列分析

• 批准号: 12671250
• 负责人: ITOI Hirosumi
• 金额: $ 2.24万
• 依托单位: Kyoto Prefectural University of Medicine(K.P.U.M)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671250/
• 关键词: MICROARRAY ANALYSIS; GENE EXPRESSION; SURGICAL TRANSPLANTATION; COLON CANCER; LIVER METASTASIS MODEL; 肝転移; 増殖・進展

The purposes of this project are the establishment of animal experiments model (liver metastases of colonic cancer) and the analyses of its gene expression using microarray analysis. The conventional technique of animal models is made by the injection of cancer cells in blood vessel or skin. In this project, the unique method was applied and made by injecting cancer cells in the subserosa of colon under surgical operation. The comparison of gene expression in both primary cancer region and metastatic region, is analyzed by micro-array assay by the m-RNA extract of cancer cells. There are still difficulties to establish the animal models, because of surgical techniques and culture cell conditions. And also there is the economical issue of the micro-array analysis cost, which is the limaitation of the number of samples.

本课题的目的是建立结肠癌肝转移动物模型，并利用基因芯片技术分析结肠癌肝转移的基因表达。传统的动物模型技术是通过将癌细胞注射到血管或皮肤中。本课题采用独特的方法，通过手术将癌细胞注射到结肠浆膜下。通过癌细胞的m-RNA提取物，通过微阵列测定来分析原发癌区域和转移区域中的基因表达的比较。由于手术技术和细胞培养条件的限制，建立动物模型仍有困难。另外，微阵列分析的成本也是一个经济问题，即样本数量的限制。

项目成果

Fujita K., Sakakura T., Shimomura K., Itoi H., Yamagishi H.: "The analysis of target genes of esophageal carcinomas detected by CGH methods"The Abstract of Japanese Society of Esophageal disease Research. 41 (2001)

Fujita K.、Sakakura T.、Shimomura K.、Itoi H.、Yamagishi H.：“CGH 方法检测的食管癌靶基因分析”日本食管疾病研究学会摘要。

下村克己他12名: "大腸癌肝転移に関連する染色体遺伝子変化の検討"Jpn J. of Cancer Research. 91・9. 69-69 (2001)

Katsumi Shimomura等12人：“与结直肠癌肝转移相关的染色体遗传变化的检查”Jpn J. of Cancer Research 91・9 (2001)。

藤田佳史他7名: "CGH法で同定された食道癌における20番染色体長腕コピー数増加と標的候補遺伝子群の解析"Japanese Society of Esopahgeal disease Research. 54・6. 41-41 (2001)

Yoshifumi Fujita等7人：“通过CGH方法鉴定的食道癌中20号染色体长臂拷贝数的增加和候选靶基因的分析”日本食管疾病研究会54・6（2001年）。

Shimomura K., Sakakura T., Fujita K., Itoi H., Yamagishi H. etc: "The analysis of chromosomal gene changes concerning the metastasis of colonic carcinomas"Jpn. J. of Cancer Research. Vol.91 Supplement. 69 (2001)

Shimomura K.、Sakakura T.、Fujita K.、Itoi H.、Yamagishi H.等：“与结肠癌转移有关的染色体基因变化的分析”Jpn。

下村 克己, 他12名: "大腸癌肝転移に関連する染色体遺伝子変化の検討"Jpn J.of Cancer Research. 91・9. 69-69 (2001)

Katsumi Shimomura等12人：“与结直肠癌肝转移相关的染色体遗传变化的检查”Jpn J.of Cancer Research 69-69(2001)。