The establishment of liver metastasis model in colonic cancer and its gene exression using microarray analyses

本课题的目的是建立动物实验模型（结肠癌肝转移），并利用微阵列分析其基因表达。传统的动物模型技术是通过在血管或皮肤中注射癌细胞来制造的。本项目采用独特的方法，在外科手术的情况下，在结肠浆膜下注射癌细胞。采用微阵列法对肿瘤细胞的m-RNA提取物进行分析，比较原发癌区和转移癌区基因表达的差异。由于手术技术和培养细胞条件的限制，建立动物模型仍然存在困难。此外，由于样品数量的限制，还存在微阵列分析成本的经济问题。

The purposes of this project are the establishment of animal experiments model (liver metastases of colonic cancer) and the analyses of its gene expression using microarray analysis. The conventional technique of animal models is made by the injection of cancer cells in blood vessel or skin. In this project, the unique method was applied and made by injecting cancer cells in the subserosa of colon under surgical operation. The comparison of gene expression in both primary cancer region and metastatic region, is analyzed by micro-array assay by the m-RNA extract of cancer cells. There are still difficulties to establish the animal models, because of surgical techniques and culture cell conditions. And also there is the economical issue of the micro-array analysis cost, which is the limaitation of the number of samples.