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Pathology and function of immuno-reactive cells for cell-mediated immunity in intestinal immune system of gastric and colorectal cancer

胃癌和结直肠癌肠道免疫系统中免疫反应细胞细胞介导免疫的病理学和功能

基本信息

批准号：
15591438
负责人：
MURAKAMI Saburo
金额：
$ 1.73万
依托单位：
Saitama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591438/
关键词：
gastric cancer colorectal cancer IL-2R IgA CD3 cell-mediated immunity cytokine マクロファージ

项目摘要

To aim to ascertain the cell-mediated immunity in gastric and colorectal cancer, we investigated the pathology and function of cel-mediated immunity, by immunohistochemical methods for the cancer and normal tissues. The immunohistochemical studies (CD25, CD3, IgA) were performed, using the surgical specimens in the thirty two cases of gastric cancer. CD25 positive cells were identified in the cancer tissues and metastatic lymph nodes, whereas they were not recognized in normal and non-metastatic lymph nodes. The same results had been recognized in twenty eight cases of the colorectal cancer. Based on the results described the above, the excistence of tumor-infiltrating lymphocytes and activated T lymphocytes were identified in gastric and colorectal cancer. Moreover, the difference between epithelial cells of normal and cancer tissues was recognized. The intraepithelial lymphocyte(IEL) was stained by CD25 in the epithelial cells of normal tissues, whereas it was not stained in cancer t … More issues. The immunohistochemical study by IgA showed the large amount of IgA near the surface of epithelial cells in normal tissues, whereas it did not show the IgA in cancer tissues. In addition, the immunohistochemical study by CD3 revealed that CD3 positive cells were recognized both in normal and cancer tissues. According to these data, the immunological function of intraepithelial lymphocytes and mucosal barrier in cancer tissues were destroyed by cancer cells, although the activated T-lymphocytes were largely identified in cancer cells. Moreover, The Levels of serum IL-12 in 143 cases with gastric cancer showed higher than those of normal controls. The immunohistochemical study by CD68 showed that the localization and distribution of macrophages were destroyed in the gastric cancer tissues. The same result was also recognized in the colorectal cancer tissues. Therefore, the origin of high levels of serum IL-12 was not derived from macrophages in the cancer tissues. Moreover, the immunohistochemical study by CD4 showed that CD4-positive cells were identified in normal tissues. On the other hand, CD4-positive cells were not recognized in gastric and colorectal tissues. Based on these data, the destruction of helper/inducer T lymphocytes was supposed in cancer tissues. Less

为了确定胃癌和结直肠癌中的细胞介导的免疫，我们通过癌症和正常组织的免疫组织化学方法研究了细胞介导的免疫的病理学和功能。使用 32 例胃癌的手术标本进行免疫组织化学研究（CD25、CD3、IgA）。 CD25阳性细胞在癌组织和转移淋巴结中被识别，而在正常和非转移淋巴结中未被识别。在 28 例结直肠癌病例中也得到了同样的结果。基于上述结果，在胃癌和结直肠癌中鉴定出肿瘤浸润淋巴细胞和活化T淋巴细胞的存在。此外，还认识到正常组织和癌组织的上皮细胞之间的差异。正常组织上皮细胞中的上皮内淋巴细胞 (IEL) 被 CD25 染色，而癌症中则不被染色。 IgA免疫组化研究显示，正常组织中上皮细胞表面附近有大量IgA，而癌组织中未见IgA。此外，CD3的免疫组织化学研究表明，CD3阳性细胞在正常组织和癌组织中均被识别。根据这些数据，尽管癌细胞中大量发现了活化的T淋巴细胞，但癌组织中上皮内淋巴细胞和粘膜屏障的免疫功能被癌细胞破坏。此外，143例胃癌患者血清IL-12水平高于正常对照。 CD68免疫组化研究显示胃癌组织中巨噬细胞的定位和分布被破坏。在结直肠癌组织中也发现了相同的结果。因此，高水平血清IL-12的来源并非来自癌组织中的巨噬细胞。此外，CD4的免疫组织化学研究表明，在正常组织中发现了CD4阳性细胞。另一方面，CD4阳性细胞在胃和结直肠组织中未被识别。根据这些数据，推测癌组织中辅助/诱导 T 淋巴细胞被破坏。较少的