Significance of E2-EPF in esophageal cancer

E2-EPF在食管癌中的意义

• 批准号: 15591445
• 负责人: ISHIBASHI Yoshio
• 金额: $ 1.98万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591445/
• 关键词: E2-EPF; Esophageal Carcinoma; Ubiquitin

1)Gene expression profilingUsing oligonucleotide microarrays, we analyzed total RNA expression levels corresponding to 12,600 transcript sequences in 24 paired cancerous and normal tissue operative specimens from 12 patients with esophageal squamous cell carcinoma. Hierarchical clustering analysis using gene expression ratios divided the 12 patients into two groups. This analysis showed significant difierenoes in the outcomes. The expression of E2-EPF was up-regulated in the poor prognostic cluster.2)ImmunoblottingEsophageal cancer samples and KYSE 50 were analyzed by SDS-PAGE, followed by immunoblotting using an anti-E2-EPF polyclonal antibody of ab4504,J8,and J9. A single hand of 24kDa was present in KYSE 50, not in esophageal cancer samples by using ab4504. But a single band of 48kDa was present in KYSE 50 and esophageal cancer samples by using J8. These result suggested that a double quantity body of E2-EPF might be present in esophageal cancer.3)ImmunohistochemistryUsing paraffin-embedded specimens from patients with esophageal squamous cell carcinoma, E2-EPF prontein was detected using an anti-E2-EPF polyclonal antibody of AP2120b using a dextrau polymer conjugate two step visualization system. E2-EPF was enriched on the surface of nuclei in cancer cells. E2-EPF protein expression patterns within cancerous lesions were nat associated with any of the following variables : TNM classification, stage, invasion into lymphatic or blood vessels, intramural metastasis, or bistologic grades of differentiation.

1)基因表达谱利用寡核苷酸芯片，我们分析了12例食管鳞状细胞癌患者的24对癌组织和正常组织手术标本中对应于12，600个转录物序列的总RNA表达水平。使用基因表达比率的层次聚类分析将12名患者分为两组。该分析显示了结果中的显著差异。E2-EPF的表达在预后不良的群体中上调。2）免疫印迹通过SDS-PAGE分析食管癌样品和KYSE 50，然后使用抗E2-EPF多克隆抗体ab 4504、J8和J 9进行免疫印迹。通过使用ab 4504，KYSE 50中存在24 kDa的单手，而食管癌样品中不存在。而KYSE 50和食管癌组织中J8均出现一条48 kDa的条带。免疫组化应用抗E2-EPF多克隆抗体AP 2120 b，采用葡聚糖二步法显色系统，检测食管鳞癌组织中E2-EPF蛋白的表达。E2-EPF在癌细胞核表面富集。E2-EPF蛋白在癌性病变中的表达模式与以下变量无关：TNM分类、分期、淋巴管或血管浸润、壁内转移或分化的生物学分级。

项目成果

Profiling gene expression ratios of paired of cancerous and normal tissue predicts relapse of esophageal squamous cell carcinoma

配对癌组织和正常组织的基因表达比率分析可预测食管鳞状细胞癌的复发

• 发表时间: 2003
• 期刊: Cancer Research 63
• 作者: Ichikawa W;Takahashi T;Suto K;Nihei Z;Shirota Y;Shimizu M;Sasaki Y;Hirayama R.;Ishibashi Y. et al.
• 通讯作者: Ishibashi Y. et al.

Ishibashi Y., et al.: "Profiling gene expression of paired of cancerous and normal Tissue predicts relapse of esophageal squamous cell carcinoma"Cancer Research. 63. 5159-5164 (2003)

Ishibashi Y. 等人：“分析癌组织和正常组织的基因表达可预测食管鳞状细胞癌的复发”癌症研究。