Suppressive effect of hepatocyte growth factor on graft-versus-host disease in nonmyeloablative preparative regimen bone marrow transplantation for pancreatic carcinoma.

本研究旨在探讨HGF（肝细胞生长因子）在非清髓预备方案骨髓移植治疗胰腺癌中对GVHD（移植物抗宿主病）的抑制作用。在原计划中，HGF对GVHD的抑制作用是通过减少免疫抑制剂的剂量来确定的。但由于T细胞和B细胞的缺乏，人们认为无法确定HGF是否可能减少免疫抑制剂。所以，我们考虑了实验室模型。我们在SCID/B6小鼠背部皮下设置癌细胞植入部位。在这个模型中，我们确定了HGF对GVHD的影响和GVT的作用。移植胰腺癌细胞后，移植骨髓和脾脏淋巴细胞。我们还测定了移植肿瘤大小的变化以及GVHD对小肠和肝脏的影响。我们的GVT效应数据是分散的，GVT效应难以量化。虽然还需要更多的实验模型来确定，但已经证实HGF可能具有降低GVHD而不降低GVT效应的可能性。

This study was designed to investigate the suppressive effect of HGF(hepatocyte growth factor) on GVHD(graft-versus-host disease) in nonmyeloablative preparative regimen bone marrow transplantation as the treatment of pancreatic cancer.In original plan, the effect of HGF on GVHD suppression was determined by dose reduction of immunosuppressant agents. But it was thought to unable to determine whether the reduction of immunosuppressant agents was possible by HGF because of the deficiency of T cell and B cell.So, we considered the laboratory model. We set the site of inplant of the cancer cell subcutaneous of the back of the SCID/B6 mouse. In this model, we determined the effect of HGF on GVHD and GVT effect.After plantation of pancreatic cancer cells, bone marrow and lymphocytes of spleen were transplanted. And we determined the changes in the size of implanted tumor and GVHD effects on small intestine and liver.Our data of GVT effect were scattering, and it was difficult to quantification of GVT effects. Although t is necessary to determine in more number of experimental model, it was confirmed that HGF may have possibilities to reduce the GVHD without reduction of GVT effect.