Establishment of the rat glioma model at the midbrain and screenig of the tumor suppressor function

由于人类固有脑干胶质瘤的治疗仍然具有挑战性，因此需要实验胶质瘤模型。我们开发了一种大鼠内源性脑干胶质瘤模型，该模型使用立体定向框架固定头部，以便通过永久植入的套管将C6胶质瘤细胞运送到目标部位。我们用悬浮在1 μl培养基中的5 × 10^4个细胞接种大鼠中脑、脑桥或大脑皮层，接种时间为2min。植入3天后，在中脑导水管周围灰质和脑桥被盖可见肿瘤形成。第10天肿瘤直径大于2mm；长度，没有肿瘤细胞播种到脑脊液间隙。肿瘤表现为胶质母细胞瘤的组织学特征；Ki-67标记指数为32%。因为在我们的模型中，套管是永久植入的，所以可以提供额外的接种。最后，我们建立了包括中脑在内的脑干的生殖胶质瘤模型，该模型具有立体定向精度。

As the treatment of human intrinsic brainstem gliomas remains challenging, experimental glioma models are needed. We developed a rat model of intrinsic brain stem glioma that uses a stereotactic frame to fix the head for the delivery of C6 glioma cells to target sites via a permanently implanted cannula. We inoculated the rat midbrain, pons, or cerebral cortex with 5 x 10^4 cells suspended in 1 μl of culture medium over the course of 2 min. Three days post-implantation, tumor formation was visible in the periaqueductal gray matter in the midbrain and in the tegmentum of the pons. On the 10^<th> day, the tumor diameter exceeded over 2 mm ; length, there was no tumor-cell seeding into the cerebrospinal fluid space. The tumor manifested the histological features typical of glioblastoma ; Ki-67 labeling index was 32%. Because in our model the cannula is permanently implanted, additional inocula can be delivered. Finally, we establish the reproductive glioma model at the brain stem including midbrain with stereotactically accuracy.