Establishment of the rat glioma model at the midbrain and screenig of the tumor suppressor function

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As the treatment of human intrinsic brainstem gliomas remains challenging, experimental glioma models are needed. We developed a rat model of intrinsic brain stem glioma that uses a stereotactic frame to fix the head for the delivery of C6 glioma cells to target sites via a permanently implanted cannula. We inoculated the rat midbrain, pons, or cerebral cortex with 5 x 10^4 cells suspended in 1 μl of culture medium over the course of 2 min. Three days post-implantation, tumor formation was visible in the periaqueductal gray matter in the midbrain and in the tegmentum of the pons. On the 10^<th> day, the tumor diameter exceeded over 2 mm ; length, there was no tumor-cell seeding into the cerebrospinal fluid space. The tumor manifested the histological features typical of glioblastoma ; Ki-67 labeling index was 32%. Because in our model the cannula is permanently implanted, additional inocula can be delivered. Finally, we establish the reproductive glioma model at the brain stem including midbrain with stereotactically accuracy.