权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Basic study for sonodynamic therapy to the experimental brain tumor

实验性脑肿瘤声动力治疗的基础研究

基本信息

批准号：
17591540
负责人：
FUKUSHIMA Takeo
金额：
$ 2.24万
依托单位：
Fukuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We confirmed efficacy of sonodynamic therapy to the experimental brain tumor in the rat. C6 glioma cells were implanted in the brain of Wistcr rat and evaluated efficacy of sonodynamic therapy using a focused ultrasound and Rose Bengal as photosensitizer. A selective anti-tumor effect against cerebral gloma while sparing normal brain tissues was achieved by sonodynamic focused therapy consisting of focused ultrasound at 25 W/cm2 at 1 MHz for 5 min and Rose Bengal, In vitro, C6 rat glioma cells and U251 and U105 human glioma cells were used and evaluated in the efficacy of sonodynamic therapy using parallel ultrasound with photofrin as a photosensitizer Usefulness of low-intensity ultrasound for the cell killing of malignant glioma was confirmed and anti-tumor activity might be enhanced by combination with photofrin, which partially transported by cell surface LRP/ α 2-MR In EM study, swelling of the cytoplasm, plenty of pinocytosis and interruption of the cytoplasmic membrane of the en … More dothelial cells were observed, suggesting-destruction of the blood brain barrier-of the-peritumoral-brain tissue by the sonodynamic therapy. In this experiment, human glioma cells, U251-and U105, differed in sensitivity to the sonodynamic therapy U251 glioma cells survived in the nude rat brain but didn't grow up to neoplastic mass. These cells might differ in proliferation individually, depending upon each animal. U105 glioma cells neither survived in the nude rat brain nor grew up to the mass, so these glioma cells couldn't be used as experimental brain tumor model. We used U87 human glioma cells for experimental brain tumor in the nude rat. These cells were implanted into the nude rat brain-successfully and grew up-to the mass in a week, so U87 human. glioma cells were used for sonodynamic-therapy using the-focused ultrasound. The nude rat brain was exposed and was irradiated by the focused ultrasound after disinfection, A week later, the tumor maximum diameter was compared ultrasound group with control group, 40 mm in average in the former and 38 mm in the latter, therefore, effectiveness of sonodynamic therapy could not be obtained in this model. We speculated that there were any differences of proliferation activity between C6 glioma cells and human glioma cells of U251, U105 and U87 and /or of accumulation rate of photosensitizer to these glioma cells. Less

我们证实了声动力疗法对实验性大鼠脑肿瘤的疗效。将C6胶质瘤细胞植入SD大鼠脑内，并使用聚焦超声和虎红作为光敏剂评价声动力学治疗的效果。通过声动力学聚焦治疗实现了对脑血管球的选择性抗肿瘤作用，同时保留正常脑组织，所述声动力学聚焦治疗由在25 W/cm 2、1 MHz下聚焦超声5分钟和虎红组成，在体外，使用C6大鼠胶质瘤细胞和U251和U105人胶质瘤细胞，并评价使用平行超声的声动力学治疗的功效，其中Photofrin作为光敏剂，低强度超声波对恶性胶质瘤细胞有杀伤作用，并与光敏素联合应用，可增强其抗肿瘤活性，光敏素部分通过细胞表面LRP/ α 2-MR转运。电镜观察可见，细胞胞浆肿胀，胞饮增多，胞膜中断，胞浆内有大量的胞饮，胞浆内有大量的胞饮细胞，胞浆内有大量的胞饮细胞，胞浆内有大量的胞饮细胞，胞浆内有大量的胞饮细胞。 ...更多信息观察到内皮细胞，通过声动力学治疗破坏了瘤周脑组织的血脑屏障。在本实验中，人胶质瘤细胞U251-和U105对声动力疗法的敏感性不同，U251胶质瘤细胞在裸大鼠脑中存活，但没有生长成肿瘤块。这些细胞的增殖可能因动物而异。U105胶质瘤细胞在裸鼠脑内不能存活，也不能生长成瘤，不能作为实验性脑肿瘤模型。我们用U87人脑胶质瘤细胞在裸鼠体内进行实验性脑肿瘤的研究。这些细胞被植入到裸大鼠的大脑中，并在一周内成功地生长到肿块，所以U87人。神经胶质瘤细胞用于使用聚焦超声的声动力学疗法。暴露裸鼠脑组织，消毒后用聚焦超声照射，1周后比较超声组和对照组肿瘤最大直径，前者平均40 mm，后者平均38 mm，说明该模型不能获得声动力学治疗的效果。我们推测C6胶质瘤细胞与U251、U105和U87胶质瘤细胞的增殖活性和/或光敏剂对这些胶质瘤细胞的蓄积率存在差异。少