Patho-parmacological Studies for Vascular Eendothel in Osteonecrosis of Femoral Head in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP).

对人股骨头坏死（ONFH）的研究表明，类固醇激素相关的骨质疏松症和氧化应激诱导的内皮功能障碍之间有密切的平行关系140只雄性SHRSP/Ngsk，在15周龄时，随机分为4组。第一组为对照组。第二组为HFC饲料组，饲喂HFC饲料。第三组为激素组，饲喂SP饲料，同时注射激素。第四组是类固醇激素加HFC饮食组，给予HFC饮食并用类固醇激素治疗。19周龄时断头处死，HFC饲料（68%）、SP饲料（船桥农场有限公司）、5%胆固醇、2%胆酸、25%牛油粉和0.006%NG-硝基-L-精氨酸（NOS抑制剂），17周龄时背部肌肉注射类固醇激素甲基强的松龙5 mg。苏木精-伊红染色观察股骨头坏死，Tunnel法检测股骨头细胞凋亡，抗8-OHdG免疫组化染色观察股骨头坏死（8-羟基脱氧鸟苷）和抗4-HNE结果表明，类固醇激素对股骨头坏死有明显的治疗作用，不仅在骨骺，而且在坏死周围的骨干中也有细胞凋亡，激素加HFC饮食组股骨头骨髓中可能存在较多的氧化应激，尤其是股骨头周围的动脉。提示氧化应激可能是引起股骨头缺血性坏死的原因之一，本研究为人类股骨头缺血性坏死的发生提供了氧化应激和高血压的参与。

Studies for human osteonecrosis of the femoral head(ONFH) have shown that a close parallelism between steroid hormone-related hyperlipemia and oxidization stress-induced endothelium dysfunction140 male SHRSP/Ngsk, maintained and bred at our university.At 15 weeks of age, rats were grouped to four at random. The first group was the control group. The second group was the HFC diet group, was fed the HFC diet. The third group was the steroid hormone administrated group, was fed the SP diet, and steroid hormone was injected. The fourth group was the Steroid Hormone plus HFC diet group, was given the HFC diet and treated with a steroid hormone. These rats were decapitated at 19th weeks of age.The regime of the HFC diet is consisted of 68%, SP diet (Funabashi Farm Co ltd),5% Cholesterol,2% Cholic acid,25% Beef tallow powder and 0.006% NG-nitro-L-arginine as a NOS inhibitor.As a steroid hormone,5mg Methyl-predonisolon was injected into the dorsal muscular layer at 17th weeks of age. Hematoxylin-Eosin staining for the check about ONFH, Tunnel's method to examine the apoptosis in the femoral head and immunostaining with anti 8-OHdG(8-hydroxy-deoxyguanosine) and anti 4-HNE(4-hydroxy-2-nonenal) were performed as pathological examinations.The study indicated that steroid hormone was very effective of osteonecrosis.Apoptosis was found not only in the femoral epiphysis but in the shaft around the necrosis, where the much adipocyte degeneration was found. So the adipocyte degeneration was suggested migration to necrosis.More oxidation stress might be existed in the bone marrow of femoral head in the steroid hormone plus HFC diet group, especially at the arteries around femoral head. It suggests that the oxidation stress may trigger the ischemic change.This study contributed the participation of hyperlipemia and oxidization stress related to the cause of femoral head avascular necrosis in humans.