权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The osteonecrosis of femoral head in stroke positive prone of spontaneously hypertensive rats, especially about the participation of fat cells

自发性高血压大鼠脑卒中阳性易发生股骨头坏死，尤其是脂肪细胞的参与

基本信息

批准号：
18591666
负责人：
KUMAGAI Kenji
金额：
$ 2.49万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The epidemiology. and the clinical approach. remarkable.. has advanced recently.. in the idiopathic osteonecrosis of the femoral head (ONFH). ONFH is found to be increased by administering more than 12.5mg of steroid hormone, hyperlipidemia, and to clarify a relationship with arteriole.. vein and endothelial cell. In recent years, there has been an increase in the incidence of steroid-induced ONFH associated with increased rates of organ transplantation and treatment of collagen disease, posing a social problem.The purpose of the research is the pathogenetic analysis of the ischemic mechanism in idiopathic osteonecrosis of the femoral head. The apractical aim was clarifying the relationship between the precapillary circulation, especially participation of the vascular endothelium andthe fat cell hyperplasia generated by the steroid hormone administering.About 100 SHRSP/Izm rats were sacrificed at the age of 17 weeks for the above-mentioned condition analysis. The femoral head were gath … More ered. The pathological specimen were made for the microscope, blood were collected, and the many internal organs were collected too.The osteonecrosis was checked microscopically by each femoral head. The qualitative reactiveness of adiponectin, PAI-1, leptin and, TNF about intra and inter fat cells surrounding the fat cell in the marrow was confirmed by using anti-rat's antibody in the immunohistology. Aquantitative reactiveness of various cytokines was evaluated by the presence of the Steroid Hormone administering and the presence of ONFH.To exam the cytokine at molecular level, it is necessary to extract from fat cell in femoral shaft of femoral head optical micro specimen. The extraction was tried to fail technically.Then, two experiments that administered the pentosan, heparin-like substance and pravastatin as a Statin were studied to check the dynamic state of the fat cell.In both study, frequency of osteonecrosis decreased, and the numberr and size of the fat cell were reducted.. The harvest was obtained by the viewpoint of the participation proof of ONFH with the fat cell and the search for the treatment of ONFH Less

流行病学。和临床方法。卓越的。最近有进展。特发性股骨头坏死（ONFH）。发现ONFH增加通过给予超过12.5mg的类固醇激素，高脂血症，并阐明与小动脉的关系。静脉和内皮细胞。近年来，激素性股骨头坏死的发病率随着器官移植和胶原病治疗率的增加而增加，成为一个社会问题，本研究的目的是探讨特发性股骨头坏死的缺血机制。本研究的目的是为了阐明毛细血管前循环，尤其是血管内皮的参与与类固醇激素引起的脂肪细胞增生之间的关系。取股骨头， ...更多信息有了。制作病理标本，留取血液标本，并取多个脏器标本，每只股骨头镜检骨坏死情况。免疫组化证实了脂联素、派-1、瘦素、TNF对骨髓脂肪细胞周围的脂肪细胞内、间的定性反应。通过激素治疗和股骨头坏死的存在来定量评价各种细胞因子的反应性，为了在分子水平上检测细胞因子，有必要从股骨头的股骨干中提取脂肪细胞。然后，我们进行了两项实验，即给予戊聚糖、肝素样物质和普伐他汀作为他汀，以检查脂肪细胞的动态状态，在这两项研究中，骨坏死的频率降低，脂肪细胞的数量和大小减少。从脂肪细胞参与股骨头坏死的证据和寻找股骨头坏死的治疗方法的观点上取得了收获。