Investigation for bone mass and trabecular bone turnover through transient regulation of marrow osteogenic and osteoclastogenic potentials follow by climbming exercise

通过短暂调节骨髓成骨和破骨细胞潜能并随后进行攀爬运动来研究骨量和小梁骨转换

• 批准号: 15591610
• 负责人: OKIMOTO Nobukazu
• 金额: $ 1.86万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591610/
• 关键词: osteoclast; bone formation rate; cortical bone; mineralized nodule; alkaline phosphatase; voluntary exercise; osteoblast; climbming

Introduction : The relationship between bone mass gain and local bone formation and resorption in mechanically loaded bone are not well understood.Material and method : 65 C57BL/6J mice, 8 weeks of age, were assigned to 5 groups : a baseline control and two groups each of ground control and climbing exercise mice for 2 and 4 weeks. Mice were housed in a 100-cm tower and had to climb to drink water toward a bottle placed at the top.Results : Compared to the ground control, bone mineral density (BMD) of the left femur increased in the climbing mice at 4 weeks. At 2 and 4 weeks, bone formation rate (BFR/BS) of periosteal surface, the cross-sectional area, and moment of inertia were increased in the climbing mice, while BFR/BS and eroded surface (ES/BS) of endosteal surface did not differ. The trabecular bone volume (BV/TV) of the proximal tibia increased in climbing mice and their osteoclasts surface (Oc.S/BS) and osteoclast number decreased at 2 weeks. At 4 weeks, there were increases in BV/TV and parameters of bone formation including mineralized surface, mineral apposition rate and bone formation rate. In marrow cell cultures from the tibia, the number of alkaline phosphatase-positive colony forming units-fibroblastic and the area of mineralized nodule formation in climbing mice were increased, and the number of osteoclast like tartrate-resistant acid phosphatase-positive multinucleated cells was lower at 2 weeks. At 4 weeks, these parameters of marrow cell cultures recovered to the levels of the ground controls.Conclusion : Our results indicated that climbing increased trabecular bone volume and reduced bone resorption, with a subsequent increase in bone formation. Intermittent climbing downregulate marrow osteoclastogenic cells and upregulate osteogenic cells initially, but further exercise appeared to desensitize them. Cortical envelopes were enlarged earlier, but the response seems to differ from trabecular bone.

简介：骨量增加与机械负载骨中局部骨形成和吸收之间的关系尚不清楚。材料和方法：65 只 8 周龄的 C57BL/6J 小鼠被分配到 5 组：一组为基线对照，两组分别为地面对照和攀爬运动小鼠，为期 2 周和 4 周。小鼠被安置在一个 100 厘米高的塔中，必须爬向顶部的瓶子喝水。结果：与地面对照相比，攀爬小鼠的左股骨骨密度 (BMD) 在第 4 周时有所增加。第2周和第4周时，攀爬小鼠的骨膜表面骨形成率（BFR/BS）、横截面积和转动惯量均增加，而骨内膜表面的BFR/BS和侵蚀表面（ES/BS）没有差异。攀爬小鼠的胫骨近端骨小梁体积（BV/TV）增加，破骨细胞表面（Oc.S/BS）和破骨细胞数量在2周时减少。第 4 周时，BV/TV 和骨形成参数（包括矿化表面、矿物质沉积率和骨形成率）有所增加。在胫骨骨髓细胞培养物中，攀爬小鼠中碱性磷酸酶阳性集落形成单位-成纤维细胞的数量和矿化结节形成面积增加，而破骨细胞样抗酒石酸盐酸性磷酸酶阳性多核细胞的数量在2周时较低。 4 周时，骨髓细胞培养物的这些参数恢复到地面对照的水平。结论：我们的结果表明，攀爬增加了小梁骨体积并减少了骨吸收，随后骨形成增加。间歇性攀爬最初会下调骨髓破骨细胞并上调成骨细胞，但进一步的运动似乎会使它们变得不敏感。皮质包膜较早扩大，但反应似乎与骨小梁不同。