Effects of delta-opioid on neuronal injury after spinal cord ischemia

在大鼠脊髓缺血模型上观察δ-阿片受体激动剂SNC 80对脊髓缺血后神经元损伤的影响。使用2 Fr Fogarty导管进行脊髓缺血，通过抽血进行低血压。研究一：SNC 80对脊髓缺血9 min和11 min后神经元损伤的影响。在脊髓缺血前15分钟鞘内施用SNC 80。结果表明，鞘内注射SNC 80可明显减轻脊髓缺血9 min和11 min大鼠灰质神经元的损伤。研究2：在大鼠脊髓缺血模型中评价脊髓缺血后白色物质损伤的变化。根据空泡化和淀粉样前体蛋白免疫反应性的程度评价白色物质损伤。结果表明，脊髓缺血后灰质和白色均受到损伤。研究三：在常温和低温下观察SNC 80对大鼠脊髓缺血后灰质和白色损伤的影响。结果表明，SNC 80在常温和低温条件下显著减轻白色损害，而其对灰质损害的作用仅在常温条件下观察到。

The effects of delta-opioid agonist SNC80 on neuronal injury after spinal cord ischemia were evaluated in rats. Spinal ischemia was conducted using 2 Fr Fogarty catheter with hypotension by blood withdrawal. SNC80 was administered intrathecally.Study 1: The effects of SNC80 on neuronal injury after 9min and 11 min of spinal cord ischemia. SNC80 was intrathecally administered 15 min prior to spinal cord ischemia. The results indicated that intrathecal administration of SNC80 significantly attenuated the neuronal injury in the gray matter in rats subjected to 9 and 11 min of spinal cord ischemia.Study 2: Changes in white matter injury after spinal cord ischemia were evaluated in a rat model of spinal cord ischemia. White matter injury was evaluated based on the extent of vacuolation and amyloid precursor protein immnoreactivity. The results indicated that both gray and white matter were injured after spinal cord ischemia.Study 3: The effects of SNC80 on gray and white matter injury after spinal cord ischemia were evaluated in rats under normothermia and hypothermia. The results indicated that SNC80 significantly attenuated white matter damage under normothermia and hypothermia, whereas its effects on gray matter damage were only noted under normothermic conditions.