Molecularbiology of the occupational urothelial tumors

第二次世界大战后，和歌山市的许多工厂生产了大量用于生产染料的芳香胺中间体。在这些工厂的员工中，尿路上皮癌的发病率一直很高。我们部门一直在对这些患者进行流行病学研究，并报告了这些癌症和尿路上皮癌在普通人群中的生物学行为的差异。然而，病理组织学研究并未显示职业性尿路上皮癌的生物学行为与普通人群的生物学行为有明显差异。1.芳香胺染整工人谷胱甘肽S转移酶M1基因的遗传研究：将染整工人分为癌症组和非癌症组，检测芳胺染整工人谷胱甘肽转移酶M1基因的多态性。有一个…GSTM1基因缺陷个体更倾向于癌症组。2.职业性尿路上皮癌中P53基因突变的研究：P53蛋白在职业性尿路上皮癌中的过度表达明显高于普通人群中的上皮性肿瘤。3.职业性尿路上皮癌中上皮细胞生长因子受体(EGFR)的表达研究：已有研究表明，EGFR在膀胱癌中的表达与肿瘤的分级和分期有关。与自发性膀胱癌相比，职业性膀胱癌组织中EGFR的过度表达。较少

After World War II, many factories in Wakayama City manufactured large quantities of aromatic amine intermediates for the production of dyes. There has been a high incidence of urothelial cancers among the employees of these factories. Our Department has been conducting epidemiological studies on these patients and reported differences in the biological behaviors of these cancers and urothelial cancers in the general population. Pathohistological studies, however, have not shown distinct differences between the biological behaviors of occupational urothelial cancers and those found in the general population. These studies investigated whether such differences could be detected using molecular biology techniques.1.Genetic studies of glutathione S-transferase M1 (GSTM1) in dye workers exposed to aromatic amines : We examined polymorphism of the GSTM1 gene among dye workers exposed to aromatic amines after dividing these workers into the cancer group and the non-cancer group. There was a … More strong tendency for GSTM1 gene-deficient individual to be in the cancer group. We also found a tendency toward an increased incidence of such gene deficiency in bladder cancer patients among dye workers compared with the incidence among bladder cancer patients in the general population.2.Investigation into the mutation of p53 gene in occupational urothelial cancers : p53 protein showed significantly higher overexpression in occupational epithelial tumors than in epithelial tumors in the general population. And also, the frequency of p53 gene mutation in occupational epithelial tumors tended to be higher than that in such tumors among the general population.3.Investigation into the expression of epithelial growth factor receptor (EGFR) in occupational urothelial cancer: It has been reported that expression of EGFR in bladder tumor participates in tumor grade and disease stage. We showed overexpression of EGFR in occupational bladder cancer compared with that in spontaneous bladder tumors. Less