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Molecularbiology of occupational urothelial tumors

职业性尿路上皮肿瘤的分子生物学

基本信息

批准号：
11671577
负责人：
SHINKA Toshiaki
金额：
$ 2.37万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671577/
关键词：
benzidine β-naphthylamine occupation GSTM1 gene p53 gene urothelial tumor dve workers moleculareoidemiology 職業性尿路上皮癌芳香族アミン p53蛋白過剰発現 p53遺伝子突然変異 DNA sequence analysis 職業性尿路上皮腫瘍免疫組織化学染色 PCR-SSCP 点突然変異

项目摘要

In this study, the differences were analyzed between labor environment factors and host specific factors related to carcinogenesis in molecularepidemiology, because some people develop cancer and others do not, even in the population exposed to many chemical carcinogens. Secondly, the differences were examined between occupational epithelial tumors and spontaneous generation epithelial tumors at the gene level.1. Examination of the Glutathion S-transferase (GSTM1) gene in dye workers exposed to aromatic amines :We examined the polymorphism of the GSTM1 gene between the cancer group and the non-cancer group, which is one of the detoxic enzymes of the aromatic amines. The abounding tendency in GSTM1 gene-deficient individual was recognized in cancer patients. In examining the relationship between occupational environment factors and the GSTM1 gene, labor environment factors, such as employment in small-scale factories and the duration of employment surpassed GSTM1 gene deficiency in carcinogenesis.2. Examination of p53 gene mutation in dye workers exposed to aromatic amines :p53 protein in occupational epithelial tumors showed an overexpression which was significantly higher than in epithelial tumors in the general population. The frequency of p53 gene mutation in occupational epithelial tumors tended to be higher than tumors in the general population.

本研究从分子流行病学的角度分析了劳动环境因素与宿主特异性致癌因素的差异，因为即使在接触多种化学致癌物的人群中，也有一些人会发生癌症，而另一些人则不会。其次，从基因水平考察职业性上皮性肿瘤与自发性上皮性肿瘤的差异。染整工人谷胱甘肽S转移酶基因的检测：我们检测了芳香胺解毒酶之一的谷胱甘肽S转移酶基因在癌症组和非癌症组之间的多态性。在癌症患者中发现GSTM1基因缺陷个体的丰富趋势。在考察职业环境因素与GSTM1基因之间的关系时，劳动环境因素，如小规模工厂的从业人数和工龄，在致癌方面超过了GSTM1基因缺陷。芳香胺作业工人P53基因突变检测：职业性上皮性肿瘤中P53蛋白过度表达，明显高于一般人群上皮性肿瘤。职业性上皮性肿瘤中P53基因突变频率高于一般人群肿瘤。