Detection of human papilloma virus(HPV) DNA by microdissection of adenocarcinoma of the uterine cervix

宫颈腺癌显微切割检测人乳头瘤病毒（HPV）DNA

• 批准号: 15591728
• 负责人: TSUNODA Hajime
• 金额: $ 1.98万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591728/
• 关键词: Cervical adenocarcinoma; Human Papilloma Virus; Laser capture microdissection; 子宮頸癌; ヒトパピローマウイルス(HPV); Laser capture microdissection

The aim of this study was to assess the correct ratio of infection of HPV and its subtype of adenocarcinoma itself, using laser capture microdissection method to eliminate the contamination of squamous cells and stromal cells. We analyzed 60 adenocarcinomas of the uterine cervix (19 endometrioid type, 37 endocervical type, 1 intestinal type, 2 poor differentiated type and 1 unclassified adenocarcinoma), and 16 of squamous epithelium around the adenocarcinoma for presence of human papillomavirus and viral subtype using laser capture microdissection method and polymerase chain reaction method.There are 21 cases with HPV of 37 endocervical types (56.8%), 15 of 19 endometrioid types (78.9%), 1 of 2 poor differentiated types (50.0%), 1 of 1 unclassified type, respectively. And there is no case with HPV in intestinal type. Totally, we found 38 cases with HPV of 60 adenocarcinomas of the cervix (63.3%). There is no case demonstrated different type between adenocarcinoma and CIN around adenocarcinoma. Our studies support that human papillomavirus may play a certain role in the adenocarcinoma pathogenesis of the uterine cervix.

本研究的目的是评估HPV及其亚型腺癌本身的感染的正确比率，使用激光捕获显微切割方法，以消除鳞状细胞和基质细胞的污染。我们分析了60例宫颈腺癌，其中类腺癌19例，宫颈腺癌37例，肠腺癌1例，低分化腺癌2例，未分类腺癌1例，应用激光捕获显微切割技术和聚合酶链反应技术对16例宫颈腺癌周围鳞状上皮进行了HPV及病毒亚型检测，共检出HPV 21例，共37个型别类分化型15例（78.9%），低分化型1例（50.0%），未分化型1例（50.0%）。肠道型HPV感染无一例。60例宫颈腺癌中HPV阳性38例，阳性率为63.3%。腺癌与癌周CIN类型无差异。我们的研究支持人乳头瘤病毒在宫颈腺癌的发病中可能起一定的作用。

项目成果

Hajime Tsunoda, Hiroyuki Yoshikawa et al.: "Ultrasound diagnosis of uterine arteriovenous fistula associated with placental site trophoblastic tumor"Ultrasound Obstet Gynecol.. 21(6). 606-608 (2003)

Hajime Tsunoda、Hiroyuki Yoshikawa 等：“与胎盘部位滋养细胞肿瘤相关的子宫动静脉瘘的超声诊断”Ultrasound Obstet Gynecol.. 21(6)。

Hajime Tsunoda, Hiroyuki Yoshikawa et al.: "Undifferentiated carcinoma with osteoclast-like multinucleated giant cells arising in an ovarian mature cystic teratoma"Gynecol Obstet Invest.. 56(4). 184-187 (2003)

Hajime Tsunoda、Hiroyuki Yoshikawa 等人：“卵巢成熟囊性畸胎瘤中出现破骨细胞样多核巨细胞的未分化癌”Gynecol Obstet Invest.. 56(4)。

Early acquisition of gelatinolytic activity in carcinogenesis of the uterine cervix.

宫颈癌发生过程中早期获得明胶分解活性。

• 发表时间: 2003
• 期刊: Mod Pathol. 16(11)
• 作者: Minami R;Tsunoda H;Iijima T;Yoshikawa H;Nemori R;Noguchi M.
• 通讯作者: Noguchi M.

Hajime Tsunoda, Hiroyuki Yoshikawa et al.: "Early acquisition of gelatinolytic activity in carcinogenesis of the uterine cervix"Moddern Pathology.. 16(11). 1164-1170 (2003)

Hajime Tsunoda、Hiroyuki Yoshikawa 等人：“在子宫颈癌发生过程中早期获得明胶分解活性”现代病理学.. 16(11)。

Early acquisition of gelatinolytic activity in carcinogenesis of the uterine cervix

宫颈癌发生过程中早期获得明胶分解活性

• 发表时间: 2004
• 期刊: Mod Pathol 16(11)
• 作者: R Minami