Detection of human papilloma virus(HPV) DNA by microdissection of adenocarcinoma of the uterine cervix

本研究的目的是评估HPV及其亚型腺癌本身感染的正确比例，采用激光捕获显微解剖方法消除鳞状细胞和基质细胞的污染。我们采用激光捕获显微解剖法和聚合酶链反应法对60例宫颈腺癌（子宫内膜样腺癌19例、宫颈内膜型37例、肠型1例、低分化型2例、未分类腺癌1例）和16例腺癌周围鳞状上皮中是否存在人乳头瘤病毒及其亚型进行分析。37种宫颈内型21例（56.8%），19种子宫内膜样型15例（78.9%），2种低分化型1例（50.0%），1种未分类型1例。没有肠道型的HPV病例。60例宫颈腺癌中有38例存在HPV，占63.3%。腺癌与腺癌周围的CIN没有不同类型的病例。我们的研究支持人乳头瘤病毒可能在子宫颈腺癌发病中起一定作用。

The aim of this study was to assess the correct ratio of infection of HPV and its subtype of adenocarcinoma itself, using laser capture microdissection method to eliminate the contamination of squamous cells and stromal cells. We analyzed 60 adenocarcinomas of the uterine cervix (19 endometrioid type, 37 endocervical type, 1 intestinal type, 2 poor differentiated type and 1 unclassified adenocarcinoma), and 16 of squamous epithelium around the adenocarcinoma for presence of human papillomavirus and viral subtype using laser capture microdissection method and polymerase chain reaction method.There are 21 cases with HPV of 37 endocervical types (56.8%), 15 of 19 endometrioid types (78.9%), 1 of 2 poor differentiated types (50.0%), 1 of 1 unclassified type, respectively. And there is no case with HPV in intestinal type. Totally, we found 38 cases with HPV of 60 adenocarcinomas of the cervix (63.3%). There is no case demonstrated different type between adenocarcinoma and CIN around adenocarcinoma. Our studies support that human papillomavirus may play a certain role in the adenocarcinoma pathogenesis of the uterine cervix.