A research for the involvement of renin-angiotensin system in placental formation

肾素-血管紧张素系统参与胎盘形成的研究

• 批准号: 15591740
• 负责人: ITAKURA Atsuo
• 金额: $ 2.24万
• 依托单位: Saitama Medical School (2005)Nagoya University (2003-2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591740/
• 关键词: angitensin II; trophoblast; extravillous trophoblast; choriocarcinoma; pregnancy induced hypertension; cytotrophoblast; 妊娠中毒症

Angiotensin II (Ang II), a bioactive peptide active in the renin-angiotensin system (RAS), has various biological functions besides its role as a potent vasoconstrictor. It has been shown to inhibit human extravillous trophoblast migration via PAI-1 activation through the Ang II type 1 receptor (AT1R). But, our previous study revealed induction of choriocarcinoma cell proliferation. The data suggested an involvement of the local RAS in tumor growth, contrasting with suppression of extravillous trophoblast invasion by RAS activation. In the present study we therefore investigated the effects of Ang II on choriocarcinoma cell migration in vitro using Boyden chamber and invasion assays. Ang II (10^<-8>M) promoted migration and invasion by a choriocarcinoma cell line (BeWo) and augmented random cell mobility on checker board analysis. Immunoblotting showed that Ang II activated the phosphorylation of FAK,ERK 1/2, and Akt in BeWo cells. Furthermore the Ang II effects on cell migration and invasion were abolished by a selective AT1R antagonist and an Akt inhibitor. The present results suggest that Ang II induced migration and invasion of choriocarcinoma cell probably involves PI3K/Akt following binding to the AT1R.

血管紧张素II（Angiotensin II，Ang II）是一种在肾素-血管紧张素系统（renin-angiotensin system，RAS）中具有活性的生物活性肽，除具有强的血管收缩作用外，还具有多种生物学功能。它已被证明可以抑制人绒毛外滋养层细胞迁移通过派-1激活通过血管紧张素II 1型受体（AT 1 R）。但是，我们以前的研究表明，诱导绒毛膜癌细胞增殖。这些数据表明，局部RAS参与肿瘤生长，与RAS激活抑制绒毛外滋养层细胞侵袭形成对比。因此，在本研究中，我们研究了血管紧张素II对绒毛膜癌细胞迁移的影响，在体外使用Boyden室和侵袭实验。Ang II（10 μ <-8>M）促进绒毛膜癌细胞系（BeWo）的迁移和侵袭，并增强棋盘分析中的随机细胞迁移率。免疫印迹显示，Ang II激活BeWo细胞中FAK、ERK 1/2和Akt的磷酸化。选择性AT 1 R拮抗剂和Akt抑制剂可阻断Ang Ⅱ对细胞迁移和侵袭的作用。提示Ang Ⅱ诱导绒癌细胞迁移和侵袭可能与其与AT 1 R结合后的PI 3 K/Akt有关。

项目成果

Possible antenatal and perinatal related factors in development of cystic periventricular leukomalacia

• DOI: 10.1016/j.braindev.2004.02.011
• 发表时间: 2005-01-01
• 期刊: BRAIN & DEVELOPMENT
• 影响因子: 1.7
• 作者: Murata, Y;Itakura, A;Mizutani, S
• 通讯作者: Mizutani, S

Angiotensin II augments migration and invasion of choriocarcinoma cells involves P13K activation through the AT1 receptor.

血管紧张素 II 增强绒毛膜癌细胞的迁移和侵袭涉及通过 AT1 受体激活 P13K。

• 发表时间: 2006
• 期刊: Placenta (in press)
• 作者: S.Ishimatsu;A.Itakura;et al.
• 通讯作者: et al.

C.Ito, A.Itakura, et al.: "A prospective evaluation of the effects of salpingectomy on endometria 1 lymphocyte clusters in cases with hydrosalpinges"Fertility and Sterility. (in press). (2004)

C.Ito、A.Itakura 等人：“输卵管积水病例中输卵管切除术对子宫内膜 1 淋巴细胞簇影响的前瞻性评估”生育力和不育。

Successful treatment of a heterotopic vical pregnancy and twin gestation by sonographically guided illation of hyperosmolar glucose

超声引导高渗葡萄糖成功治疗异位妊娠和双胎妊娠

• 发表时间: 2006
• 期刊: Acta Obstet Gynecol Scand 2006 (in press)
• 作者: Suzuki;A.Itakura;et al.
• 通讯作者: et al.

Successful treatment of a heterotopic cervical pregnancy ant twin gestation by sonographically guided instillation of hyperosmolar glucose

超声引导高渗葡萄糖滴注成功治疗异位宫颈妊娠和双胞胎妊娠

• 发表时间: 2006
• 期刊: Acta Obstet Gynecol Scand (in press)
• 作者: M.Suzuki;A.Itakura;et al.
• 通讯作者: et al.