The functional analysis of the putative choriocarcinoma suppressor gene, HTF12 in choriocarcinoma.

HTF12基因位于人类第7号染色体7q11.2，有三个cDNA，剪接变体。我检查了从该区域纯合缺失到绒毛膜癌恶性转化的干预。我回顾了是否有一个独特的序列表达，但没有发现启动子特异性，因为在绒毛膜癌提取物中的表达分析是困难的，我确定了在正常绒毛中表达的表达位点。原位杂交证实滋养细胞转变为合体滋养细胞，并表达HTF12。未转化为合体滋养细胞的滋养细胞未被识别。基于此，估计HTF12基因是影响合胞化的基因。此外，当加入forskolin时，合体化明显促进，HTF12在滋养层细胞中的表达增强，forskolin是一种让你改变的合体化化学物质。 ...更多信息 在绒毛细胞中进入它。在这个帐户上，有人建议，毛喉素采取行动超过HTF12。在提供的葡萄胎中，至今未发现HTF12区域的缺陷，HTF12的表达并未消失。HTF12不作用于绒毛使磨牙水肿的水平，并且建议其通过致癌水平参与转化。此外，肉眼可见的全部痣68例，其中绒毛囊肿154例为葡萄胎，部分痣为显微镜下可见的痣。然而，通过多态性分析，120例被认为是雄核发育，正是它的后果让你预见到，基因诊断的诊断被打破，为未来的宏观诊断。除所有痣外，有必要对一例贝壳癌变进行复查。没有广泛的多态性分析的数据，但到目前为止，通过多态性分析证实了日本人的多态性分布。少

The HTF12 gene is in the 7th human chromosome at 7q11.2, and has three cDNAs, splice variants.I examined intervention from this region being homozygous deletion to malignant transformation in choriocarcinoma. I reviewed whether there was an unique sequence to express, but nothing specific for promoter was not found. Because expression analysis in an extractive tissues of choriocarcinoma was difficulty, I confirmed the expression locus about expression in a normal villus. The trophoblastic cell which changed into syncitiotrophoblast, and expression of HTF12 was recognized by in situ hybridization. The trophoblastic cell which did not change into syncitiotrophoblast was not recognized. On this account that a HTF12 gene was the gene which affected syncitionization was estimated. Furthermore, the syncitionyzation was promoted obviously, and expression of HTF12 in a trophoblastic cell was enhanced when having added forskolin which was the syncitionyzation chemical material which let you cha … More nge into it in a villus cell. On this account it was suggested that forskolin acted over HTF12. Deficiency of HTF12 region was not recognized till now in a provided mole, and expression of HTF12 did not disappear. HTF12 did not act on the level that a villus turned molar edematization into, and that participated in transformation by a cancer-causing level was suggested. In addition, all moles were 68 examples macroscopically, and, among villus 154 examples that a cyst changed into it as for the hydatidiform mole, as for the part mole, a microscopical mole was had elsewhere. However, by analysis with polymorphism, it was recognized that 120 examples were androgenesis, and it was it with the consequence which let you foresee that a diagnosis by gene diagnosis was broken for the future, macroscopic diagnosis. Necessity to reexamine a case of canceration of shell besides all moles was confirmed till now. There were not data of wide polymorphism analysis, but distribution of polymorphism of a Japanese was confirmed to a Japanese till now by polymorphism analysis. Less