Isolation and Identification of Tumor suppressor gene in choriocarcinoma.

绒毛膜癌抑癌基因的分离与鉴定。

• 批准号: 11671631
• 负责人: MATSUDA Takao
• 金额: $ 2.3万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671631/
• 关键词: Villi; choriocarcinoma; placenta; tumor suppressor gene; subtraction; human chromosome7; BAC; cDNAライブラリー; 7q11.22; 造腫瘍性の抑制

1) We previously reported that the homozygous deletion in human chromosome7 were presented in choriocarcinoma tissues and cell lines. We thought the presence of tumor suppressor effect at that lesion in chromosome7. We have got the several BAC clones from the lesion located at 7q11.22 by using STS marker. Then we introduce these BAC clones into choriocarcinoma cell lines. The clones transfected with RS544-12D (B6) and RS741-8D (B7)were suppressed in vivo tumorigenicity. Then by using these clones for probe we got several cDNA library clones that suppressed the tumorigenicity of choriocarcinoma cell lines. We through that these were one of the putative choriocarcinoma suppressor genes. Now we analyze the mechanisms of tumor suppress activity of the genes.2) We made the villi-specific cDNA library that subtracted by large amount of choriocarcinoma cDNA fragment. One of the unknown gene, named clone S31 expressed only in villi, but not in choriocarcinoma. The size of clone S31 is 1.1 kb, coded 73 amino acids and have tumor suppressive potential when this clone were introduced into choriocarcinoma cell line. This S31 are homologous to gingiva expressed cDNA clone and located near the putative lesion of juvenile gingivatitts.

1)我们以前报道过人类7号染色体的纯合缺失在绒毛膜癌组织和细胞系中出现。我们认为在7号染色体的病变处存在肿瘤抑制作用。利用STS标记获得了位于7q11.22位点的几个BAC克隆。然后我们将这些BAC克隆引入绒毛膜癌细胞系。转染RS544-12D （B6）和RS741-8D （B7）的克隆在体内的致瘤性受到抑制。然后利用这些克隆作为探针，获得了几个抑制绒毛膜癌细胞系致瘤性的cDNA文库克隆。我们认为这些是一种假定的绒毛膜癌抑制基因。现在我们分析这些基因抑制肿瘤活性的机制。2)通过大量减去绒毛膜癌cDNA片段，构建绒毛特异性cDNA文库。其中一个未知基因，命名为克隆S31，仅在绒毛中表达，而在绒毛膜癌中不表达。克隆S31的大小为1.1 kb，编码73个氨基酸，导入绒毛膜癌细胞后具有抑瘤作用。该S31与牙龈表达的cDNA克隆同源，位于推定的青少年牙龈炎病变附近。

项目成果

Matsuda et al: "Molecular Biology of Choriocarcinoma. V.Basic knowledge of the malignant tumor in corpus uteri"Obstet.Gynecol (Tokyo). 66. 320-325 (1999)

松田等人：“绒毛膜癌的分子生物学。V.子宫体恶性肿瘤的基础知识”Obstet.Gynecol（东京）。

松田貴雄 他: "新女性医学体系 第37巻 絨毛性疾患 D.新知見(印刷中)"中山書店. (2000)

Takao Matsuda 等：“新女性医疗系统第 37 卷：绒毛膜疾病 D. 新发现（印刷中）”Nakayama Shoten（2000 年）。

松田貴雄 他: "8.絨毛癌の分子生物学 V.子宮体部悪性腫瘍の基礎知識"産科と婦人科. 66. 320-325 (1999)

Takao Matsuda等：“8.绒毛膜癌的分子生物学V.子宫体恶性肿瘤的基础知识”妇产科66.320-325(1999)。

Zhou Y et al: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development."Molecular Carcinogenesis. 25. 64-72 (1999)

Zhou Y等人：“DPC4启动子突变参与子宫内膜癌的发展。”分子癌发生。

Zhou Y et al.: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development"Molecular Carcinogenesis. 25. 64-72 (1999)

Zhou Y等人：“DPC4启动子突变参与子宫内膜癌的发展”分子癌发生。