Functional analysis of LH/hCG receptor in human placental chorionic villous macrophages

人胎盘绒毛膜绒毛巨噬细胞LH/hCG受体的功能分析

基本信息

  • 批准号:
    15591763
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2005
  • 项目状态:
    已结题

项目摘要

Human gonads and non-gonadal organs/tissues express LH/CG receptors. This study aimed to identify the LH/CG receptors and to clarify their function in human placental chorionic villous macrophages. Macrophages as well as syncytiotrophoblasts of human chorionic villous tissues were immunohistochemically positive for LH/CG receptor throughout gestation. By RT-PCR, villous macrophages were shown to express a variant type of LH/CG receptor, the sequencing of which revealed a deletion of exon 9. For experiments in vitro, a monocyte-macrophage cell line, THP-1, was transfected with vector alone, wild-type LH/CG receptor, and exon 9-deleted LH/CG receptor after phorbol 12-myristate 13-acetate (PMA) treatment. Non-PMA-treated THP-1 cells transfected with vector alone were also examined. THP-1 cells expressed exon 9-deleted LH/CG receptor after treatment with PMA. After the cells of the four groups were cultured in medium containing intact hCG, the concentrations of hCG and its b-core fragment … More (β-CF) were measured in the supernatant of the culture medium and in the cell cytosol. Time-dependent hCG uptake was observed in both non-PMA-treated and PMA-treated THP-1 cells, suggesting that the variant receptor is not directly involved in the ingestion of hCG. The degradation of hCG and excretion of β-CF were progressed in PMA-treated cells but not in the un-treated cells. In the cell cytosol, the ratio of β-CF and hCG concentrations (β-CF/hCG) was significantly higher in the PMA-treated cells than in non-PMA-treated cells ; however, it did not differ between the PMA-treated cells transfected with exon 9-deleted receptor and those transfected with vector alone. Macrophages may express the variant receptor in order to recognize the intracytoplasmic hCG and transport it to the lysosome. Among the two PMA-treated cells, the ratio was lower in those transfected with wild-type receptor. The expression of the variant receptor may modulate the degradation of hCG but be reduced by expression of the wild-type receptor in its lacking macrophages. Our data suggest a potentially important role for exon 9-deleted LH/CG receptors expressed in human placental villous macrophages in the local metabolism of hCG. Less
人类性腺和非性腺器官/组织表达LH/CG受体。本研究旨在鉴定人胎盘绒毛膜绒毛巨噬细胞中LH/CG受体并阐明其功能。人绒毛膜绒毛组织巨噬细胞和合体滋养细胞在整个妊娠期LH/CG受体免疫组化阳性。通过RT-PCR,绒毛巨噬细胞表达一种LH/CG受体的变异类型,其测序显示外显子9缺失。在体外实验中,将载体、野生型LH/CG受体和外显子9缺失的LH/CG受体分别转染单核巨噬细胞系THP-1,经phorbol 12-肉豆汁酸13-乙酸酯(PMA)处理。用载体单独转染未经pma处理的THP-1细胞也进行了检测。经PMA处理后,THP-1细胞表达外显子9缺失的LH/CG受体。四组细胞在含完整hCG的培养基中培养后,测定培养基上清液和细胞质中hCG及其b核片段β-CF的浓度。在非pma处理和pma处理的THP-1细胞中都观察到hCG摄取的时间依赖性,这表明变异受体不直接参与hCG的摄取。在pma处理的细胞中,hCG的降解和β-CF的排泄有进展,而在未处理的细胞中没有进展。在细胞质中,pma处理的细胞β-CF和hCG浓度之比(β-CF/hCG)显著高于未处理的细胞;然而,转染外显子9缺失受体的pma处理细胞与单独转染载体的细胞之间没有差异。巨噬细胞表达这种变异受体是为了识别胞浆内hCG并将其转运到溶酶体。在两种pma处理的细胞中,转染野生型受体的细胞比例较低。变异受体的表达可以调节hCG的降解,但在其缺失的巨噬细胞中,野生型受体的表达会减少hCG的降解。我们的数据表明,在人胎盘绒毛巨噬细胞中表达的外显子9缺失的LH/CG受体在hCG的局部代谢中具有潜在的重要作用。少

项目成果

期刊论文数量(23)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages.
  • DOI:
    10.1016/j.placenta.2004.07.001
  • 发表时间:
    2005-04
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    N. Sonoda;H. Katabuchi;H. Tashiro;T. Ohba;R. Nishimura;T. Minegishi;H. Okamura
  • 通讯作者:
    N. Sonoda;H. Katabuchi;H. Tashiro;T. Ohba;R. Nishimura;T. Minegishi;H. Okamura
Establishment of an immortalised human ovarian surface epithelial cell line without chromosomal instability
  • DOI:
    10.1038/sj.bjc.6602662
  • 发表时间:
    2005-07-11
  • 期刊:
  • 影响因子:
    8.8
  • 作者:
    Maeda, T;Tashiro, H;Okamura, H
  • 通讯作者:
    Okamura, H
Pathophysiological dynamics of human ovarian surface epithelial cells in epithelial ovarian carcinogenesis, review
人卵巢表面上皮细胞在卵巢上皮癌发生中的病理生理动力学,综述
N.Sageshima, et al.: "Necrotic feature of the trophoblasts lacking HLA-G expression in normal and preeclamptic placentas"American Journal of Reproductive Immunology. 49. 174-182 (2003)
N.Sageshima 等人:“正常和先兆子痫胎盘中缺乏 HLA-G 表达的滋养层细胞的坏死特征”美国生殖免疫学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Histologic type, organ of origin, and Wnt pathway status: effect on gene expression in ovarian and uterine carcinomas.
组织学类型、起源器官和 Wnt 通路状态:对卵巢癌和子宫癌基因表达的影响。
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KATABUCHI Hidetaka其他文献

KATABUCHI Hidetaka的其他文献

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{{ truncateString('KATABUCHI Hidetaka', 18)}}的其他基金

The development of novel therapeutic strategies for targeting ovarian cancer stem cells
针对卵巢癌干细胞的新型治疗策略的开发
  • 批准号:
    21390454
  • 财政年份:
    2009
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
An ovarian carcinoma model using immortalized human ovarian surface epithelial cells without chromosomal instability
使用无染色体不稳定的永生化人卵巢表面上皮细胞的卵巢癌模型
  • 批准号:
    18390450
  • 财政年份:
    2006
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Characterization of immortalized human ovarian surface epithelial cells transfected by PTEN expression vectors
PTEN表达载体转染的永生化人卵巢表面上皮细胞的表征
  • 批准号:
    12671616
  • 财政年份:
    2000
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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Convergent evolution of placental villi in primates and ungulates: Are some placentas more efficient than others?
灵长类动物和有蹄类动物胎盘绒毛的趋同进化:某些胎盘是否比其他胎盘更有效?
  • 批准号:
    BB/Y005953/1
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    2024
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    $ 2.24万
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Relationship among fluctose uptake, intestinal permeability, and length of intestinal villi in NAFLD patients
NAFLD患者果糖摄取量、肠道通透性和肠绒毛长度的关系
  • 批准号:
    23K06915
  • 财政年份:
    2023
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    $ 2.24万
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    Grant-in-Aid for Scientific Research (C)
Mechanisms by which trophoblasts recruit T cells to the placental villi during maternal HIV and CMV co-infection
母体 HIV 和 CMV 合并感染期间滋养层将 T 细胞募集至胎盘绒毛的机制
  • 批准号:
    10080877
  • 财政年份:
    2020
  • 资助金额:
    $ 2.24万
  • 项目类别:
Mechanisms by which trophoblasts recruit T cells to the placental villi during maternal HIV and CMV co-infection
母体 HIV 和 CMV 合并感染期间滋养层将 T 细胞募集至胎盘绒毛的机制
  • 批准号:
    10223400
  • 财政年份:
    2020
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    $ 2.24万
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Characteristic DNA methylation profles of chorionic villi in recurrent miscarriage
复发性流产中绒毛膜绒毛的特征性 DNA 甲基化谱
  • 批准号:
    19K18701
  • 财政年份:
    2019
  • 资助金额:
    $ 2.24万
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    Grant-in-Aid for Early-Career Scientists
Spatiotemporal analysis and pathogenesis of proinflammatory regulatory T cells in intestinal villi
肠绒毛促炎调节T细胞的时空分析及发病机制
  • 批准号:
    19K22624
  • 财政年份:
    2019
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Analysis of the role of laeverin expression in fetal-placental circulating stem cells derived from chorionic villi
Laeverin表达在绒毛膜绒毛来源的胎儿胎盘循环干细胞中的作用分析
  • 批准号:
    18K16793
  • 财政年份:
    2018
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    $ 2.24万
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    Grant-in-Aid for Early-Career Scientists
The identification of cystic villi-specific molecular markers in early pregnancy and their clinical significance
妊娠早期囊性绒毛特异性分子标志物的鉴定及其临床意义
  • 批准号:
    16K11144
  • 财政年份:
    2016
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    $ 2.24万
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    Grant-in-Aid for Scientific Research (C)
Analysis of chorionic villi-derived embryo stem cells by novel sten cell markers, laeberin and hTERT
通过新型干细胞标记 laeberin 和 hTERT 分析绒毛膜绒毛来源的胚胎干细胞
  • 批准号:
    16K15702
  • 财政年份:
    2016
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    Grant-in-Aid for Challenging Exploratory Research
Immunohistochemical analysis of macrophages in the placental chorionic villi for the elucidation of the pathogenesis of fetal inflammatory response syndrome
胎盘绒毛膜绒毛巨噬细胞的免疫组织化学分析阐明胎儿炎症反应综合征的发病机制
  • 批准号:
    15K20150
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    2015
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    $ 2.24万
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    Grant-in-Aid for Young Scientists (B)
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