Functional analysis of LH/hCG receptor in human placental chorionic villous macrophages

人胎盘绒毛膜绒毛巨噬细胞LH/hCG受体的功能分析

• 批准号: 15591763
• 负责人: KATABUCHI Hidetaka
• 金额: $ 2.24万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591763/
• 关键词: Placenta; Villi; Macrophage; LH-hCG receptor; exon 9-deleted LH-hCG receptor; THP-1 cell; hCG; hCG β subunit core fragment; hCGβ subunit core fragment; hCG β subunit core fragment; hCGβsubunit core fragment

Human gonads and non-gonadal organs/tissues express LH/CG receptors. This study aimed to identify the LH/CG receptors and to clarify their function in human placental chorionic villous macrophages. Macrophages as well as syncytiotrophoblasts of human chorionic villous tissues were immunohistochemically positive for LH/CG receptor throughout gestation. By RT-PCR, villous macrophages were shown to express a variant type of LH/CG receptor, the sequencing of which revealed a deletion of exon 9. For experiments in vitro, a monocyte-macrophage cell line, THP-1, was transfected with vector alone, wild-type LH/CG receptor, and exon 9-deleted LH/CG receptor after phorbol 12-myristate 13-acetate (PMA) treatment. Non-PMA-treated THP-1 cells transfected with vector alone were also examined. THP-1 cells expressed exon 9-deleted LH/CG receptor after treatment with PMA. After the cells of the four groups were cultured in medium containing intact hCG, the concentrations of hCG and its b-core fragment … More (β-CF) were measured in the supernatant of the culture medium and in the cell cytosol. Time-dependent hCG uptake was observed in both non-PMA-treated and PMA-treated THP-1 cells, suggesting that the variant receptor is not directly involved in the ingestion of hCG. The degradation of hCG and excretion of β-CF were progressed in PMA-treated cells but not in the un-treated cells. In the cell cytosol, the ratio of β-CF and hCG concentrations (β-CF/hCG) was significantly higher in the PMA-treated cells than in non-PMA-treated cells ; however, it did not differ between the PMA-treated cells transfected with exon 9-deleted receptor and those transfected with vector alone. Macrophages may express the variant receptor in order to recognize the intracytoplasmic hCG and transport it to the lysosome. Among the two PMA-treated cells, the ratio was lower in those transfected with wild-type receptor. The expression of the variant receptor may modulate the degradation of hCG but be reduced by expression of the wild-type receptor in its lacking macrophages. Our data suggest a potentially important role for exon 9-deleted LH/CG receptors expressed in human placental villous macrophages in the local metabolism of hCG. Less

人腺体和非助剂器官/组织表达LH/CG受体。这项研究旨在鉴定LH/CG受体并阐明其在人类绒毛膜绒毛巨噬细胞中的功能。在整个妊娠期间，巨噬细胞以及人绒毛膜组织的合胞素层细胞在LH/CG受体上是免疫组织化学阳性的。 By RT-PCR, villous macrophages were shown to express a variant type of LH/CG receptor, the sequencing of which revealed a deletion of exon 9. For experiments in vitro, a monocyte-macrophage cell line, THP-1, was translated with vector alone, wild-type LH/CG receptor, and exon 9-deleted LH/CG receptor after phorbol 12-myristate 13-乙酸盐（PMA）治疗。还检查了单独使用载体翻译的非PMA处理的THP-1细胞。用PMA处理后，THP-1细胞表达外显子9删除的LH/CG受体。将四组细胞的细胞在包含完整HCG的培养基中培养后，HCG及其B核片段的浓度……在培养基的上清液和细胞胞质溶胶中测量更多（β-CF）。在非PMA处理和PMA处理的THP-1细胞中都观察到时间依赖性的HCG摄取，这表明该变体受体与HCG摄入摄入无直接相关。在PMA处理的细胞中，HCG和β-CF极端的降解是在未经处理的细胞中进行的。在细胞细胞质中，PMA处理的细胞中β-CF和HCG浓度（β-CF/HCG）的比率明显高于未经PMA处理的细胞。但是，用外显子9删除的受体翻译的PMA处理的细胞与单独使用载体翻译的细胞之间没有差异。巨噬细胞可以表达变异受体，以识别胞质内HCG并将其传输到溶酶体。在两个PMA处理的细胞中，在用野生型受体翻译的细胞中，比率较低。变体受体的表达可能会调节HCG的降解，但通过野生型受体在缺乏巨噬细胞中的表达来降低。我们的数据表明，在HCG的局部代谢中，在人斑绒毛巨噬细胞中表达的外显子9删除的LH/CG受体的潜在重要作用。较少的

项目成果

Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages.

• DOI: 10.1016/j.placenta.2004.07.001
• 发表时间: 2005-04
• 期刊: Placenta
• 影响因子: 3.8
• 作者: N. Sonoda;H. Katabuchi;H. Tashiro;T. Ohba;R. Nishimura;T. Minegishi;H. Okamura

N.Sageshima, et al.: "Necrotic feature of the trophoblasts lacking HLA-G expression in normal and preeclamptic placentas"American Journal of Reproductive Immunology. 49. 174-182 (2003)

N.Sageshima 等人：“正常和先兆子痫胎盘中缺乏 HLA-G 表达的滋养层细胞的坏死特征”美国生殖免疫学杂志。

Pathophysiological dynamics of human ovarian surface epithelial cells in epithelial ovarian carcinogenesis, review

人卵巢表面上皮细胞在卵巢上皮癌发生中的病理生理动力学，综述

• 发表时间: 2005
• 期刊: International review of cytology 242
• 作者: Okamura H.;Katabuchi H.
• 通讯作者: Katabuchi H.

Pathophysiological dynamics of human ovarian surface epithelial cells in epithelial ovarian carcinogenesis.

• DOI: 10.1016/s0074-7696(04)42001-4
• 发表时间: 2005
• 期刊: International review of cytology
• 作者: H. Okamura;H. Katabuchi

Characterization of 17beta-hydroxysteroid dehydrogenase type 4 in human ovarian surface epithelial cells.

• 发表时间: 2005
• 期刊: Molecular human reproduction
• 影响因子: 4
• 作者: Y. Nagayoshi;T. Ohba;H. Yamamoto;Y. Miyahara;H. Tashiro;H. Katabuchi;H. Okamura