Study regarding effects of the radical scavenger on ischemic-induced facial palsy

自由基清除剂对缺血性面瘫的影响研究

• 批准号: 15591819
• 负责人: NAKATANI Hiroaki
• 金额: $ 2.3万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591819/
• 关键词: facial palsy; animal model of facial palsy; reactive oxygen spicies; radical scavenger; nerve regeneration

The reactivation of herpes virus in the geniculate ganglion and the secondary microvascular disturbance of the facial nerve are essential for development of acute peripheral facial palsy. Especially, a vicious cycle of nerve edema and ischemia in the facial canal is essential for deterioration of palsy. Since ischemia is well known to promote free radical reactions, reactive oxygen species (ROS) is expected to play a crucial role in the development of palsy. In this study, we investigated the incidence of palsy, production of ROS, and histological findings after use of a radical scavenger, edaravone (Radicut・).1.The incidence of facial palsy53 Hartley guinea pigs served in this study and the petrosal branch of the middle meningeal artery was obstructed to cause ischemic facial palsy. Edaravone was given to 21 animals just after obstruction of the artery and to 12 animals 2 days after obstruction. The other 20 animals were not given edaravone as controls. The incidence of palsy in the a … More nimals treated early was remarkably low as compared to control animals and the incidence in late treated animals significantly decreased after administration of edaravone.2.Production of ROSROS was measured by dihydrotetramethyirosamine probe (Molecular Probes). The production of ROS was remarkable in the facial nerve of a non-treated animal, but it was nearly normal in the facial nerve of an edaravone-treated animal.3.Histological examination of facial nerve damageHistological changes of the facial nerve were investigated 1 month after artery damage in both treated and non-treated animals. The mastoid portion of the facial nerve showed severe damage in both groups but the geniculate ganglion in edaravone-treated animals showed preservation of cell bodies and findings of nerve regeneration whereas non-treated animals still revealed severe degeneration.In this study, edaravone attenuated development of ischemia-induced facial palsy in guinea pigs and prevented the production of ROS remarkably. In conclusion, the results lead that edaravone open the new avenue to the therapy of acute peripheral facial palsy. Less

疱疹病毒在膝状神经节内的重新激活和继发性面神经微血管障碍是急性周围性面神经麻痹发生的必要条件。特别是，神经水肿和面神经管缺血的恶性循环是瘫痪恶化的关键。由于缺血是众所周知的，以促进自由基反应，活性氧（ROS）预计将在瘫痪的发展中发挥至关重要的作用。本研究观察了自由基清除剂依达拉奉（Radicut·）对豚鼠面瘫的发生率、ROS的产生以及组织学改变。1.面瘫的发生率本研究采用Hartley豚鼠53只，以脑膜中动脉岩分支为模型，通过阻断其岩部分支，造成缺血性面瘫。21只动物在动脉阻塞后立即给予依达拉奉，12只动物在阻塞后2天给予依达拉奉。另外20只动物不给予依达拉奉作为对照。瘫痪的发生率在一个 ...更多信息 与对照动物相比，早期治疗的动物的发病率显著降低，并且在给予依达拉奉后，晚期治疗的动物的发病率显著降低。2.通过二氢四甲基氨基葡萄糖探针（MolecularProbes）测量ROSROS的产生。3.面神经损伤的组织学检查动脉损伤后1个月，观察依达拉奉治疗组和对照组面神经的组织学变化。两组动物的面神经乳突部均表现出严重损伤，但依达拉奉治疗动物的膝状神经节显示细胞体保留和神经再生，而未治疗动物仍表现出严重变性。在这项研究中，依达拉奉减轻了豚鼠缺血性面神经麻痹的发展，并显着阻止了活性氧的产生。结论：依达拉奉为急性周围性面神经麻痹的治疗开辟了新的途径。少

项目成果

Effects of edaravone on ischemia-induced facial palsy

依达拉奉对缺血性面瘫的作用

• 发表时间: 2003
• 期刊: Facial Nerve Research Japan 23
• 作者: Taizo Takeda;Setsuko Takeda;Hiroaki Nakatani;Masashi Hamada;Kazuhiro Yamakawa;Masaya Takumida
• 通讯作者: Masaya Takumida

虚血性顔面神経麻痺モデル動物に与えるEdaravoneの効果

依达拉奉对缺血性面神经麻痹模型动物的作用

• 发表时间: 2003
• 期刊: Facial Nerve Research Jpn 23
• 作者: 竹田泰三;竹田節子;中谷宏章;浜田昌史;山河和博;工田昌也
• 通讯作者: 工田昌也