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Molecular biological and electrophyrsiological studies of extranodal matrices on the saltatory conduction of the optic nerve

结外基质对视神经跳跃传导的分子生物学和电生理学研究

基本信息

批准号：
15591857
负责人：
OOHASHI Toshitaka
金额：
$ 2.24万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591857/
关键词：
Bral1 Hapln2 proteoglycan hyaluronan knockout mice optic nerve

项目摘要

The Bral1/Hapln2 proteins are expressed specifically at the nodes of Ranvier in the central nervous system and supposed to be important for the maintenance of matrix molecules by stable binding on the hyaluronan. To explore the role of the extranodal matrices on the saltatory conduction, we analyzed the Bral1/Hapln2 knockout mice.1.Immunohistochemical and electronmicroscopic analysisThere was no obvious alteration on the formation of paranodes, which is consisted of glial cell (oligodendrocyte) and neuron (axon) interactions. There was also no obvious effect on the clustering of Na+ channels, which is essential for the production of action potentials and propagation of the signals.2.Electrophiological analysisHowever, when we compared the speed of propagation of action potentials on the optic nerves via electrophysiological measurement, there was a significant difference between wild type and Bral1/Hapln2 deficient mice. To figure out those differences, we investigated further the diff … More erences of the extranodal matrices as well as the attachment of the endofoot of astrocyte at the node. We could find the mislocalization of several ECM molecules including versican (a chondroitin sulfate proteoglycan), tenascin-R and others in the Bral1/Hapln2 deficient mice. During those analyses, we could find one ECM molecule, which has not reported so far to localize at the nodes of Ranvier, is colocalizing and stabilized with Bral1/Hapln2. Nevertheless, there was no obvious difference of the attachment of the endofoot of the astrocyte.3.Possible physiological roles of the extranodal matrixCollectively, there was no obvious difference in formation of paranodes and astrocyte foot process via glial and neuronal cell interactions. We could only observe the difference in the formation of the structure of extranodal matrices. Therefore, we assume that the complex of matrix molecules consisting of chondroitin sulfate proteoglycans and other glycoprotein is contributing to the maintenance of the microenvironment around the nodes. Possibly, it can serve as a Na+ ion-pool, which is important for generating the action potentials via influx through the voltage dependent Na+ channels at the nodes. Less

Bral 1/Hapln 2蛋白在中枢神经系统的郎维氏结特异性表达，并被认为是通过稳定结合透明质酸来维持基质分子的重要蛋白。为探讨突触基质在跳跃传导中的作用，我们对Bral 1/Hapln 2基因敲除小鼠进行了分析。1.免疫组织化学和电镜分析由胶质细胞（少突胶质细胞）和神经元（轴突）相互作用组成的副阳极的形成没有明显改变。对Na+通道的聚集也没有明显的影响，这是产生动作电位和信号传播所必需的。2.电生理学分析然而，当我们通过电生理测量比较视神经上动作电位的传播速度时，野生型和Bral 1/Hapln 2缺陷型小鼠之间存在显著差异。为了找出这些差异，我们进一步研究了 ...更多信息结节基质的形成以及星形胶质细胞的内足在结节处的附着。我们可以在Bral 1/Haplin 2缺陷小鼠中发现几种ECM分子的错误定位，包括多功能蛋白聚糖（一种硫酸软骨素蛋白聚糖）、腱生蛋白R等。在这些分析过程中，我们可以发现一种ECM分子，其迄今为止尚未报道定位于Ranvier结，与Bral 1/Hapln 2共定位并稳定。3.神经节基质的可能生理作用总体而言，通过胶质细胞和神经元细胞的相互作用，在副阳极和星形胶质细胞足突的形成方面没有明显差异。我们只能观察到节点矩阵结构形成的差异。因此，我们假设由硫酸软骨素蛋白聚糖和其他糖蛋白组成的基质分子复合物有助于维持淋巴结周围的微环境。可能，它可以作为Na+离子池，这对于通过节点处的电压依赖性Na+通道的流入产生动作电位是重要的。少

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Lp3/Hapln3, a novel link protein which colocalizes with versican and is coordinately upregulated by platelet-derived growth factor in arterial smooth muscle cells

Lp3/Hapln3，一种新型连接蛋白，与多功能蛋白聚糖共定位，并在动脉平滑肌细胞中被血小板衍生生长因子协同上调

DOI：
发表时间：
2004
期刊：
Matrix Biol. 23
影响因子：
0
作者：
Ogawa H;Oohashi T;Sata S;Bekku Y;Hirohata S;Nakamura K;Yonezawa T;Kusachi S;Shiratori Y;Ninomiya Y
通讯作者：
Ninomiya Y

Rauch et al.: "Cartilage link protein interacts with neurocan, which shows hyalurona binding characteristics different from CD44 and TSG-6"Matrix Biol.. (in press). (2004)

Rauch 等人：“软骨连接蛋白与神经聚糖相互作用，显示出与 CD44 和 TSG-6 不同的透明质酸结合特征”Matrix Biol..（正在出版）。