Analysis of genetic polymorphisms associated with viral infection

病毒感染相关基因多态性分析

• 批准号: 15591867
• 负责人: NAKAO Kumiko
• 金额: $ 1.66万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591867/
• 关键词: uveitis; polymorphisms; Behcet's disease; HTLV-1; NOS; SOD; TNF-α; LT-α; ウイルス; ICAM-1

To elucidate whether any polymorphic genes for antioxidant enzymes and cytokines are associated with the development of uveitis, a case-control study was performed on a consecutive series of 191 patients with uveitis (51 patients with Vogt-Koyanagi-Harada disease, 78 patients with Behcet's disease, 62 patients with HTLV-1-associated uveitis). Controls were 88 systemically healthy individuals who had no inflammatory ocular disorders. The polymorphisms of endothelial nitric oxide synthase(eNOS)、manganese superoxide dismutase(MnSOD)、extracellular superoxide dismutase(EC-SOD), intercellular adhesion molecule-1(ICAM-1)、tumor necrosis factor-α(TNF-α)、TNF receptor、and lymphotoxin-α(LT-α) were analyzed by the method of PCR restriction fragment length polymorphism. The genotype frequency distributions of eNOS, EC-SOD, and ICAM-1 did not show a statistically significant difference between the patients and controls. We found a significant association of MnSOD-9valine/alanine polymorphism with Behcet's disease and HAU(p=0.00005,p=0.05). The patients had an increased frequency of valine allele and valine/valine genotype. Serum levels of MnSOD were 18,1±3.5 ng/ml in VKH, 22.3±4.8 ng/ml in Behcet's disease, and 20.4±6.3 ng/ml in HAU. Serum levels of MnSOD in Behcet's disease were significantly increased, but they were not associated with MnSOD polymorphism. We also found a significant association of TNF-α-863 and LT-α polymorphism with Behcet's disease. The patients had an increased frequency of TNF-α-863 A allele(p=0.05), and they had an increased frequency of A allele and A/A genotype in LT-α(p=0.0024,p=0.0007). The genotype frequency distributions of TNF-α-1031,-308,-238, and TNF receptor did not show a statistically significant difference. These results suggested that MnSOD, TNF-α-863, and LT-α polymorphisms might be related to etiology of Behcet's disease.

为了阐明抗氧化酶和细胞因子的任何多态性基因是否与葡萄膜炎的发展相关，对191例葡萄膜炎患者（51例Vogt-Koyanagi-Harada病患者，78例Behcet病患者，62例HTLV-1相关葡萄膜炎患者）进行了连续系列的病例对照研究。对照组为88名全身健康的个体，他们没有炎症性眼部疾病。采用PCR限制性片段长度多态性方法分析内皮型一氧化氮合酶（eNOS）、锰超氧化物歧化酶（MnSOD）、细胞外超氧化物歧化酶（EC-SOD）、细胞间粘附分子-1（ICAM-1）、肿瘤坏死因子-α（TNF-α）、肿瘤坏死因子受体（TNF-α）和光敏素-α（LT-α）基因多态性。eNOS、EC-SOD和ICAM-1基因型频率分布在患者组和对照组之间差异无统计学意义。我们发现MnSOD-9缬氨酸/丙氨酸多态性与白塞病和HAU显著相关（p= 0.00005，p =0.05）。患者的缬氨酸等位基因和缬氨酸/缬氨酸基因型频率增加。血清MnSOD水平VKH为18.1 ± 3.5ng/ml，Behcet病为22.3± 4.8ng/ml，HAU为20.4± 6.3ng/ml。白塞病患者血清MnSOD水平明显升高，但与MnSOD基因多态性无关。我们还发现TNF-α-863和LT-α基因多态性与白塞病有显著关联。TNF-α-863 A等位基因频率增高（p=0.05），LT-α A等位基因和A/A基因型频率增高（p= 0.0024，p =0.0007）。TNF-α-1031、-308、-238和TNF受体基因型频率分布差异无统计学意义。提示MnSOD、TNF-α-863和LT-α基因多态性可能与白塞病的发病有关。

项目成果

Analysis of HLA class I and class II gene polymorphisms in Japanese patients with human T-cell lymphotropic virus type 1-associated uveitis.

日本人类T细胞嗜淋巴细胞病毒1型相关葡萄膜炎患者HLA I类和II类基因多态性分析

• 发表时间: 2005
• 期刊: Ocular Immunology and Inflammation 15
• 作者: Kaminagayoshi T;Nakao K;et al.
• 通讯作者: et al.

Kaminagayoshi T, Nakao K, et al.: "Analysis of HLA class I and class II gene polymorphisms in Japanese patients with human T-cell lymphotropic virus type 1-associated uveitis"Ocular Immunology and Inflammation. in press. (2004)

Kaminagayoshi T、Nakao K 等人：“人类 T 细胞嗜淋巴细胞病毒 1 型相关葡萄膜炎日本患者 HLA I 类和 II 类基因多态性分析”眼部免疫学和炎症。