Research of transplantation of the cryopreserved tracheal allograft

冻存同种异体气管移植的研究

基本信息

批准号：
15591886
负责人：
MAEDA Kosaku
金额：
$ 1.66万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Background : Transplantation of cryopreserved tracheal allograft is thought to be an effective therapeutic procedure of the congenital tracheal stenosis. However, the long-term prognosis is not clear. Especially, the influence of immune reaction is not understood well. We evaluated the morphologic changes of the cryopreserved tracheal allograft in using radiation or immunosuppression in a growing rabbit model.Methods : Each allograft was harvested from 90- to 120-day-old Japanese rabbits, immersed in the preservation solution, and stored in a programmable freezer until reaching -80℃ and then kept in liquid nitrogen for one month. Orthotopic tracheal transplantation of 4 tracheal rings in an end-to-end fashion was performed in age-matched young rabbits. Seven animals were classified into three groups : the group used radiation (3OGy) of the graft before transplantation, the group that used Tacrolimus as immunosuppressant and control. All grafts were evaluated 4 to 8 weeks after transplantation.Results : One animal died from technical problem. The body weight gain was similar in three groups. All grafts were patent. Microscopic findings of the allograft with radiation showed remarked fibrosis in the subepithelium. The development of a neovascularization around the graft was showed in Tacrolimus group.Conclusions : There was no acute rejection in three groups. Radiation to transplanted allograft developed severe fibrosis. Tacrolimus induced remarked angiogenesis around the allograft. Chronic rejection was not obviously observed in this animal model.

背景：冷冻保存的气管同种异体移植被认为是先天性气管狭窄的有效治疗方法。但是，长期进步尚不清楚。特别是，免疫反应的影响不太了解。我们评估了在不断增长的兔子模型中使用辐射或免疫抑制的冷冻保存气管同种异体移植的形态变化。方法：每种合金从90天到120天的日本兔子收获，沉浸在保存中，并保存在可编程的冷冻室中，直到将其存放在-80 liquies for-80 liquies in in in norrogen中。以年龄匹配的年轻兔子进行了对4个气管环的原位气管移植。将七只动物分为三组：移植前使用了移植物的辐射（3ogy），该组将他克莫司作为免疫抑制剂和对照组。移植后4至8周对所有移植物进行了评估。回报：一只动物死于技术问题。三组体重增加相似。所有移植物都是专利。辐射的同种血管化的显微镜发现显示了上皮下纤维化。他克莫司组显示了围绕移植物周围的新血管形成的发展。结论：三组没有急性排斥。移植合金的辐射会出现严重的纤维化。他克莫司诱导的同种异体移植周围的血管生成。在这种动物模型中并未明显观察到慢性排斥。