Application of tissue regeneration using acellular scaffolds by technique of regenerative medicine

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To develop a regenerative trachea of stable and safe mechanical strength, we attempted the making acellular tracheal scaffold and its application i.e., the tracheal reconstruction by using acellular tracheal scaffold.Dog or rabbit trachea enucleated were rinsed by Triton X-100 for 24 hours at ambient temperature. The component cells in trachea were removed almost all by Triton X-100 treatment. Rabbit and dog trachea were replaced by the acellular tracheal matrix lyophilized and sterilized. Acellular tracheal scaffold of rabbit kept the function of tracheal duct after grafting. Twenty-eight numbers of rabbit grafted survived for 14 to 60 days post surgery. However, all of rabbits grafted died by acellular trachea replaced was infected. Accelular tracheal scaffold of dog also kept the enough function of tracheal duct after grafting. Rabbit grafted acellular trachea did not show angiogenesis at grafted region enough but dog grafted accellular trachea obtained the results of good angiogenesis. The cellular infiltration and connective tissue proliferation in accellular trachea was not only found but also bronchial epithelium attracted on the lumen of acellular trachea grafted 2-months post grafting histochemically.Acellular tracheal scaffold may be an effective regenerative device for tracheal reconstruction.