Study of the adhesion prevention with using self-setting barrier membrane

自凝阻隔膜防粘连的研究

• 批准号: 12470310
• 负责人: INOUE Hajime
• 金额: $ 9.09万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470310/
• 关键词: alginate membrane; adhesion; self-setting barrier membrane; osteocyte; surgical treatment; laminectomy; phagocytosis; 骨細胞

Alginate membrane is easily prepared with sodium alginate and potassium chloride. This material is easy to handle and is used in several fields. The present study focus on the preventing role of this self-setting barrier membrane in adhesion formation.In vivo study, the effect of alginate membrane during wound healing was investigated in Wistar rats. Rat paravertebral muscle was exposed on its back to establish an adhesion model. Alginate membrane was applied to the cavity over the muscle abrasion. Histological assessment showed that alginate membrane occupied the cavity fully and inflammation cells infiltrated in the first week after operation. After 3 week, giant cells came up to the edge of this membrane. Alginate membrane was almost absorbed by the phagocytosis induced by giant cells within 9 week. Compared to the control group, the granulation tissue, fibrosis and the organization of remodeling tissue were reduced. Furthermore, fat tissue replacement was well recognized in alginate membrane treated group. In another study, the prevention of neural adhesion was also investigated in Beagle dogs. Alginate membrane was applied after laminectomy. Similar histological findings were observed in epidural space and around neural tissue, to reduce adhesion with using this membrane. Taken together these data, this bioresorbable membrane has the effect to diminish the adhesion after operation.We also examined the effect of the alginate in vitro study. In the study with the osteocyte, the significant difference was not found in cell proliferation. The function of osteocyte (the production of osteocalcin and ALP) was not damaged by this membrane.Alginate membrane is forein material and the further examinations are needed for the control of inflammation and absorption. However alginate membrane is though to be one of the suitable materials for the prevention of adhesion after surgical treatments.

以海藻酸钠和氯化钾为原料，制备了海藻酸钠膜。这种材料易于处理，并用于多个领域。本研究的重点是这种自固化屏障膜在粘连形成中的预防作用。在体内研究中，藻酸盐膜在Wistar大鼠伤口愈合过程中的作用进行了研究。背部暴露大鼠椎旁肌建立粘连模型。将藻酸盐膜应用于肌肉擦伤上方的空腔。术后1周组织学检查显示藻酸盐膜完全占据腔体，炎细胞浸润。3周后，巨细胞长至膜边缘。藻酸盐膜在9周内几乎被巨细胞诱导的吞噬作用所吸收。与对照组相比，肉芽组织减少，纤维化程度减轻，重塑组织机化程度减轻。此外，藻酸盐膜处理组的脂肪组织替代良好。在另一项研究中，还在比格犬中研究了神经粘连的预防。椎板切除术后应用藻酸盐膜。在硬膜外腔和神经组织周围观察到类似的组织学结果，以减少使用该膜的粘连。综合这些数据，这种生物可吸收膜具有减少术后粘连的效果。我们还在体外研究中检查了藻酸盐的效果。在骨细胞的研究中，未发现细胞增殖的显著差异。海藻酸钠膜对骨细胞的功能（骨钙素和碱性磷酸酶的产生）无明显影响，是一种较有前途的材料，其抗炎和吸收作用有待进一步研究。然而，藻酸盐膜被认为是用于预防手术治疗后粘连的合适材料之一。