Bone regeneration of allogeneic bone marrow derived mesenchymal cells in femoral segmental model

股骨节段模型中同种异体骨髓间充质细胞的骨再生

• 批准号: 15591913
• 负责人: TSUCHIDA Hiroyuki
• 金额: $ 1.79万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591913/
• 关键词: bone regeneration; bone marrow derived mesenchymal cell; BMP rat; femoral segmental model; XY-FISH; allograft; 骨形成因子; BMP2; 間葉系幹細胞; mesenchymal stem cell; FISH; allograft; bone regeneration; 骨形成

Lewis male rat was used as donor and Brown Norway female rat (allogeneic transplantation model) and Lewis female rat (syngeneic transplantation model) was used as the recipient. Three-dimensional culture by the collagen gel was done after rhBMP 2 was added to MSC that was gathered from the donor femoral bone and cultured. Then it transplanted recipient's femoral bone defect. Recipient rats were divided three groups. A and B groups were allogeneic transplantation model (different condition of FK506), and group C was syngeneic transplantation model. A group (n=20) administered FK506 (1mg/1kg weight) one every day of the first week of after the operation, and, in addition, went in the every other day for one week. B group (n=20) administered FK506 (10mg/1kg weight) in single times on that day of the operation. C group (n=8) did not use FK506. The amount of the bone formation was evaluated by the soft X-ray on 2, 4, 6, and eight weeks after operation and the histological evaluation was don … More e. Transplanted cells were evaluated by fluorescence in situ hybridization (FISH) for survival time and the part where it lives.All three groups were obtained excellent bone formations. In the histology evaluation, woven bone was observed at two weeks after the operation and at six weeks after the operation lamellar bone was observed.The amount of bone formation was used six point scale of the soft X-ray image, a statistics and significant difference was not admitted between three groups. B group's transplanted cells where it had lived were less than A group at 4-6 weeks after the operation were little.In rat allogeneic MSC transplantation using of the rhBMP 2, although single use of FK506 group had few transplant living cell at 4-6 weeks than two week use of FK506 group, single use of FK506 group obtained the same degree of the bone formation as the two week use of FK506 group. It was thought that transplanted cells were thought to be participation only in a bone formation early phase of post implantation. We demonstrated a long-term immune suppression is unnecessary, even if MSC was transplanted under the allogeneic condition. Less

刘易斯雄性大鼠作为供体，Brown Norway雌性大鼠（异基因移植模型）和刘易斯雌性大鼠（同基因移植模型）作为受体。将rhBMP 2加入骨髓间充质干细胞（MSC）中进行体外培养，胶原凝胶三维培养。然后移植受者股骨缺损。将大鼠分为3组。A、B组为异基因移植模型（不同FK 506条件），C组为同基因移植模型。一组（n=20）在术后第一周每天给予FK 506（1 mg/1 kg体重）一次，此外，每隔一天给药一次，持续一周。B组（n=20）于手术当天单次给予FK 506（10 mg/kg体重）。C组（n=8）不使用FK 506。分别于术后2、4、6、8周行软X线片检查，并进行组织学观察 ...更多信息 e.荧光原位杂交（FISH）检测移植细胞的存活时间和存活部位，三组均获得良好的成骨效果。组织学评价：术后2周可见编织骨，术后6周可见板层骨，成骨量采用软X线片六点评分法，三组间无统计学差异。B组术后4- 6周移植活细胞数少于A组，rhBMP 2组大鼠同种异体MSC移植中，单用FK 506组4-6周移植活细胞数虽少于2周组，单用FK 506组骨形成程度与单用FK 506两周组相同。认为移植的细胞仅参与植入后的骨形成早期阶段。我们证明了长期的免疫抑制是不必要的，即使是在同种异体条件下移植MSC。少