Functional Analysis of Dentin Matrix Protein 1 (DMP1) and Regulation Analysis of their Expression during Fracture Healing.

牙本质基质蛋白 1 (DMP1) 的功能分析及其在骨折愈合过程中的表达调控分析。

• 批准号: 15591930
• 负责人: TOYOSAWA Satoru
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591930/
• 关键词: dentin matrix protein 1; mineralization; osteocyte; fracture healing; transgenic mouse; mRNA expression; protein localization; osteoporosis; 発現制御; 歯; 骨

Dentin matrix protein 1 (DMP1) is one of the acidic phosphorylated extracellular matrix proteins called the SIBLING (small integrin-binding ligand, N-linked glycoproteins) family. Recent studies showed that DMP1 is expressed in the mineralized tissues and suggested that DMP1 is involved in the mineralization. We investigated the precise localization of DMP1 messenger RNA (mRNA) and protein during fracture healing. In situ hybridization demonstrated that DMP1 mRNA was strongly expressed in preosteocytes and osteocytes in the bony callus during intramembranous and endochondral ossification. However, DMP1 mRNA was not detected in osteoblasts and chondrocytes. During endochondral ossification, however, a low number of DMP1-expressing cells were identified in the cluster of hypertrophic chondrocytes. However, these DMP1-expressing cells were not hypertrophic and were likely to be osteoblast-lineage cells, which were embedded in the matrix of bone or cartilage, because type I collagen-expressing cells and invasion of capillary vessels were observed in the same area. Northern blot, in situ hybridization, and immunohistochemical analyses showed that DMP1 mRNA and protein expressions were increased until day 14 postfracture, when bony callus was formed, and then declined to a lower level during remodeling of the bony callus. Therefore, DMP1 is likely to play an important role in the mineralization of the bony callus. Secondly, we elucidated the effect of DMP1 during fracture healing in DMP1-transgenic mice. X-ray and histological analysis showed no differences during fracture healing between in wild and transgenic mice. Thirdly, we estimated the DMP1 expression and distribution in ovariectomy-induced osteoporosis rats. Immunohistochemical studies showed no change of DMP1 expression and distribution in the osteoporosis rats.

牙本质基质蛋白1（Dentin matrix protein 1，DMP 1）是一种酸性磷酸化的细胞外基质蛋白，被称为SIBLING（small integrin-binding ligand，N-linked glycoproteins）家族。近年来的研究表明，DMP 1在矿化组织中表达，提示DMP 1参与了矿化过程。我们研究了骨折愈合过程中DMP 1信使RNA（mRNA）和蛋白质的精确定位。原位杂交结果表明，膜内和软骨内骨化过程中，DMP 1 mRNA在骨痂的前骨细胞和骨细胞中强烈表达。而成骨细胞和软骨细胞中未检测到DMP 1 mRNA。然而，在软骨内骨化过程中，在肥大的软骨细胞群中发现了少量的DMP 1表达细胞。然而，这些表达DMP 1的细胞不是肥大的，并且可能是成骨细胞系细胞，其嵌入骨或软骨的基质中，因为在同一区域中观察到I型胶原表达细胞和毛细血管的侵袭。北方印迹、原位杂交和免疫组化分析显示，骨折后14天，当骨痂形成时，DMP 1 mRNA和蛋白表达增加，然后在骨痂重塑过程中下降至较低水平。因此，DMP 1可能在骨痂的矿化过程中发挥重要作用。其次，我们阐明了DMP 1在DMP 1转基因小鼠骨折愈合过程中的作用。X射线和组织学分析显示，在野生型和转基因小鼠之间的骨折愈合过程中没有差异。第三，我们检测了去卵巢骨质疏松大鼠模型中DMP 1的表达和分布。免疫组化结果显示，骨质疏松大鼠骨组织中DMP 1的表达和分布无明显变化。

项目成果

Expression of dentin matrix protein 1 in tumors causing

牙本质基质蛋白1在肿瘤中的表达引起

• 发表时间: 2004
• 期刊: Modern Pathology 17
• 作者: 中野 裕紀子;豊澤 悟;山本 慎治;豊澤 悟;Shinji Yamamoto et al.;Satoru Toyosawa et al.;Satoru Toyosawa et al.;中野 裕紀子;豊澤 悟;豊澤 悟
• 通讯作者: 豊澤 悟

Expression level of valosin-containing protein(VCP)…

含缬洛辛蛋白（VCP）的表达水平...

• 发表时间: 2004
• 期刊: Ann.Oncol. 15
• 作者: 中野 裕紀子;豊澤 悟;山本 慎治;豊澤 悟;Shinji Yamamoto et al.;Satoru Toyosawa et al.;Satoru Toyosawa et al.;中野 裕紀子;豊澤 悟;豊澤 悟;山本 慎治
• 通讯作者: 山本 慎治

Eccentric localization of osteocytes expressing enzymatic activities, protein, and mRNA signals for type 5 tartrate-resistant acid phosphatase (TRAP)

• DOI: 10.1369/jhc.4a6378.2004
• 发表时间: 2004-11-01
• 期刊: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
• 影响因子: 3.2
• 作者: Nakano, Y;Toyosawa, S;Takano, Y
• 通讯作者: Takano, Y

豊澤 悟: "mRNA expression and protein localization of…"Bone. 34. 124-133 (2004)

Satoru Toyosawa：“……的 mRNA 表达和蛋白质定位” Bone. 34. 124-133 (2004)

Expression of Dentin Matrix Protein 1(DMP1) during Fracture Healing.

骨折愈合过程中牙本质基质蛋白 1 (DMP1) 的表达。

• 发表时间: 2004
• 期刊: Bone 35
• 作者: Takeyasu;K.;Kim;J.;Ohniwa;R.;L.;Kobori;T.;Morikawa;K.;Ohta;T.;Ishihama;A.;Yoshimura;S.H.;S.Toyosawa
• 通讯作者: S.Toyosawa