The regeneration of tooth in combination with homologous transplantation of freezed tooth germ and non-freezed bone marrow tissue.

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Very few researches were available on the transplantation of immature tooth germs at the initial stage of hard tissue formation into intraalveolar tissue, although similar studies on the transplantaion of those tissues into the capsule of kidney or the subcutaneous tissues of the back skin. Initially, cell morphology and lectin immunohistochemistry were examined in freezed tooth germ with light microscopy, to explore whether preservation of tooth germ by freezing could make any influence on various cellular components of tooth germ. Simultaneously, light microscopic staining of compound polysaccharides were carried out to observe these polysaccharides which exists abundantly at the intercellular tissue.The rat tooth germ of upper first molar was preserved by freezing at postnatal 5 day, thawed after one week, and histology and the expression of compound polysaccharides were compared with non-freezed control tooth germ on ameloblasts, cells of the intermediate layer, stellate cells of e … More namel pulp, odontoblasts, pulp cells and vascular endothelial cells. Subsequently, histology, lectin expression and staining of compound polysaccharides showed no definite difference in most of cellular and intercellular components between the experimental group and the control, suggesting that preservation by freezing made no significant changes in tooth germ tissue.Consequently, tooth germs at postnatal 5 days were taken from the half number of siblings, and preserved by freezing, and transplanted into the jaw bone of the remaining siblings at postnatal 13 days, and the capacity of transplanted tooth germ to form hard tissue was explored histologically at postoperative one and two weeks. Moreover staining of sugar chains and compound polysaccharides were also compared with the unfrozen control group. Newly formed dent inogenesis was scatteringly observed at postoperative one week, and the dentin became thick at postoperative 2 weeks. Sugar chains as well as compound polysaccharides showed no definite deviation in expression and localization of pulp cells and odontoblasts between the experimental and control group. These above findings suggested that frozen tooth germs maintained dentinogenesis. Resorption of enamel or osseous ankylosis, both of which could occur in the trauma of tooth germ, was observed in those frozen tooth germs. The prevention of these changes is open to be solved hereafter The combination of tooth germ transplantation with the implantation of bone marrow tissue from the tibia formed slightly thicker dentin, although the residual bone marrow could not be confirmed after the implantation and such marrow tissue could be associated with the improved dentinogenesis, if any. Less