The regeneration of tooth in combination with homologous transplantation of freezed tooth germ and non-freezed bone marrow tissue.

结合冷冻牙胚和非冷冻骨髓组织同源移植的牙齿再生。

• 批准号: 15591960
• 负责人: TANIGUCHI Kunihisa
• 金额: $ 1.86万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591960/
• 关键词: regeneration of tooth germ; preservation by freezing; transplantation; sugar chain; lectin stainin; bone marrow transplantation

Very few researches were available on the transplantation of immature tooth germs at the initial stage of hard tissue formation into intraalveolar tissue, although similar studies on the transplantaion of those tissues into the capsule of kidney or the subcutaneous tissues of the back skin. Initially, cell morphology and lectin immunohistochemistry were examined in freezed tooth germ with light microscopy, to explore whether preservation of tooth germ by freezing could make any influence on various cellular components of tooth germ. Simultaneously, light microscopic staining of compound polysaccharides were carried out to observe these polysaccharides which exists abundantly at the intercellular tissue.The rat tooth germ of upper first molar was preserved by freezing at postnatal 5 day, thawed after one week, and histology and the expression of compound polysaccharides were compared with non-freezed control tooth germ on ameloblasts, cells of the intermediate layer, stellate cells of e … More namel pulp, odontoblasts, pulp cells and vascular endothelial cells. Subsequently, histology, lectin expression and staining of compound polysaccharides showed no definite difference in most of cellular and intercellular components between the experimental group and the control, suggesting that preservation by freezing made no significant changes in tooth germ tissue.Consequently, tooth germs at postnatal 5 days were taken from the half number of siblings, and preserved by freezing, and transplanted into the jaw bone of the remaining siblings at postnatal 13 days, and the capacity of transplanted tooth germ to form hard tissue was explored histologically at postoperative one and two weeks. Moreover staining of sugar chains and compound polysaccharides were also compared with the unfrozen control group. Newly formed dent inogenesis was scatteringly observed at postoperative one week, and the dentin became thick at postoperative 2 weeks. Sugar chains as well as compound polysaccharides showed no definite deviation in expression and localization of pulp cells and odontoblasts between the experimental and control group. These above findings suggested that frozen tooth germs maintained dentinogenesis. Resorption of enamel or osseous ankylosis, both of which could occur in the trauma of tooth germ, was observed in those frozen tooth germs. The prevention of these changes is open to be solved hereafter The combination of tooth germ transplantation with the implantation of bone marrow tissue from the tibia formed slightly thicker dentin, although the residual bone marrow could not be confirmed after the implantation and such marrow tissue could be associated with the improved dentinogenesis, if any. Less

将处于硬组织形成初期的未成熟牙胚移植到牙槽内组织的研究很少，但将这些组织移植到肾被膜或背部皮肤皮下组织的研究却很少。首先，用光镜观察冷冻牙胚的细胞形态和凝集素免疫组化，以探讨牙胚冷冻保存是否会对牙胚的各种细胞成分产生影响。将大鼠上颌第一磨牙牙胚于生后5d冷冻保存，1周后解冻，与未冷冻对照牙胚比较成釉细胞、中间层细胞、E星状细胞 ...更多信息 牙髓、成牙本质细胞、牙髓细胞和血管内皮细胞。随后，组织学、凝集素表达和复合多糖染色显示，实验组与对照组之间的大部分细胞和细胞间成分无明显差异，表明冷冻保存对牙胚组织无明显影响。于出生后13天将牙胚移植到其余同胞的颌骨中，并于术后1周和2周对移植牙胚形成硬组织的能力进行组织学观察。并与解冻对照组比较糖链和复合多糖的染色情况。术后1周可见散在的新牙本质形成，术后2周牙本质增厚。实验组和对照组牙髓细胞和成牙本质细胞中糖链和复合多糖的表达和定位无明显差异。上述结果表明，冷冻牙胚能维持牙本质的形成。牙胚冷冻后可出现牙胚损伤后牙釉质吸收或骨性强直。牙胚移植联合胫骨骨髓组织植入后，牙本质形成较厚，但植入后不能确认骨髓残留，骨髓组织可能与牙本质形成有关。少

项目成果

歯胚の凍結保存による糖鎖発現および硬組織形成への影響

牙胚冷冻保存对糖链表达和硬组织形成的影响

• 发表时间: 2005
• 期刊: 福岡歯科大学学会雑誌 31
• 作者: 江原道子