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Study on time-dependent variability in response properties found in mammalian taste neurons using sieve electrodes.

使用筛电极研究哺乳动物味觉神经元响应特性随时间变化的变化。

基本信息

批准号：
15591979
负责人：
SHIMATANI Yuichi
金额：
$ 2.11万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591979/
关键词：
Gustatory System Chorda Tympani Nerve Regeneration Chronic Recording Micromachine Sieveelectrode

项目摘要

The receptor cells of taste buds have a life span of about 10 days but it is not known if the response characteristics of these receptors alters during this turnover. To examine taste cell responses over time, a micromachined polyimide sieve electrode array was implanted between the cut ends of the rat chorda tympani nerve. We made the new version of sieve electrodes that have Iridium metal surface on each recording site. With this change, stability of recordings and expansion of recording period were achieved. Long-term stable recordings from regenerated single afferent fibers innervating taste buds were possible using this technique for up to a few weeks. Taste stimuli recorded from the same single fiber changed with time. The changes occurred in both the magnitude of response and the relative response profiles to four chemical stimuli, NaCl, sucrose, HCl and quinine HCl. These changes in response characteristics were hypothesized to result from changes in the taste receptor cells innervated by the afferent fiber as the receptor cells turn over in the taste buds.Our results might pose a significant question regarding the theories of coding of taste quality since the electrophysiological data on which these theories have been based has been obtained in acute single fiber recordings nearly all of which are from cut decentralized nerves. Since the response characteristics change from day to day how is the information of taste quality is maintained? For example, based on the results of the chronic recordings tomorrow's "sweet pattern" will be different from today's "sweet pattern". Further experiments will hopefully confirm these findings and provide new information on the cell biology of the taste receptors.

味蕾的受体细胞的寿命约为10天，但不知道这些受体的反应特性是否在这种转换过程中发生变化。为了检查随着时间的推移的味觉细胞的反应，微加工的聚酰亚胺筛电极阵列被植入大鼠鼓索神经的切断端之间。我们制作了新版本的筛电极，在每个记录位点上具有铱金属表面。通过这种变化，实现了记录的稳定性和记录周期的扩展。长期稳定的记录从再生的单一传入纤维支配味蕾是可能的，使用这种技术长达几个星期。从同一根纤维记录的味觉刺激随时间而变化。对NaCl、蔗糖、HCl和盐酸奎宁四种化学刺激的反应幅度和相对反应曲线都发生了变化。这些反应特征的变化被认为是由于受传入纤维支配的味觉感受器细胞在味蕾中翻转时发生了变化。我们的结果可能对味觉编码理论提出了一个有意义的问题，因为这些理论所基于的电生理数据是在急性单纤维记录中获得的，几乎所有的记录都来自切割。分散神经由于响应特性每天都在变化，如何保持味觉质量的信息？例如，基于慢性记录的结果，明天的“甜蜜模式”将不同于今天的“甜蜜模式”。进一步的实验将有望证实这些发现，并提供有关味觉受体细胞生物学的新信息。