Invitromineral induction by phosphoprpteins-Change of PH in mineral induction

磷酸蛋白体外矿物质诱导-矿物质诱导中 PH 的变化

• 批准号: 15592027
• 负责人: TOYOOKA Hiroki
• 金额: $ 2.05万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592027/
• 关键词: mineral induction; PH; induction activity

We previously reported that immobilized phosphoprpteins and insoluble dentin were able to induce to mineral formation in metastable solution. However, it were not reported pH change that immobilized phosphoprotein induce apatite formation. In this study, phosphoproteins were crosslinked to an agarose beads and measured pH at several time in metastable solution. We investigated that usefulness of evaluation using electrical engineering in mineral induction. Phosvitin was used as a model phosphoprotein in this study. Phosvitin was immobilized to agarose beads. Three mineralizing solutions were used. Ten microliters of each sample were incubated in 3ml of metastable solutions at 37℃ in a shaker. Samples were measured pH at several time points and filtered. Then, samples were analyzed for calcium by atomic absorption spectrometry. Phosvitin crosslinked agarose beads were observed fall of pH 2.5h, 3h, 5h after beginning of incubation in the three solution. At the same time, Samples increased in analysis for calcium absorption spectrometry. These results suggested this method was more simple and precision.

我们以前报道过，固定化磷酸蛋白和不溶性牙本质能够在亚稳态溶液中诱导矿物形成。然而，没有报道固定化磷蛋白诱导磷灰石形成的pH变化。在这项研究中，磷蛋白交联的琼脂糖珠，并在几个时间在亚稳溶液中测量pH值。我们研究了在矿物感应中使用电气工程评估的有用性。在这项研究中，卵黄高磷蛋白被用作模型磷蛋白。将卵黄高磷蛋白固定于琼脂糖珠。使用了三种矿化溶液。在37℃下，在振荡器中，将10 μ L各样品置于3 ml亚稳态溶液中孵育。在几个时间点测量样品的pH并过滤。然后，通过原子吸收光谱法分析样品中的钙。在三种溶液中孵育开始后2.5h、3h、5h观察到卵黄高磷蛋白交联琼脂糖珠的pH下降。与此同时，钙吸收光谱法分析的样品增加。结果表明，该方法简便、准确。

项目成果

in vitro study of Dentin : Effect of Ions an Mineral Induction by Decalcified

牙本质的体外研究：离子对脱钙矿物质诱导的影响

• 发表时间: 2003
• 期刊: Caries Research 212
• 作者: Takashi Saito;Hiroki Toyooka;Shinichi Ito;Miles A.Crenshaw
• 通讯作者: Miles A.Crenshaw

Takasi Saito, Hiroki Toyooka, Shuichi Ito, Miles A.Crenshaw: "In vitro Study of Dentin : Effect of Ions on Mineral Induction by Decalcified Dentin Matrix."Caries Research. 212. 1-5 (2003)

Takasi Saito、Hiroki Toyooka、Shuichi Ito、Miles A.Crenshaw：“牙本质的体外研究：离子对脱钙牙本质基质矿物质诱导的影响。”龋齿研究。

in vitro study of Dentin : Effect of Ions on Mineral Induction by Decalcified

牙本质的体外研究：离子对脱钙矿物诱导的影响

• 发表时间: 2003
• 期刊: Caries Research 212
• 作者: Takasi Saito;Hiroki Toyooka;Shuiti Ito;MilesA. Crenshaw
• 通讯作者: MilesA. Crenshaw