Effect of ions on in vitro mineral induction by dentin matrix

修复材料释放的离子对牙本质矿化的影响尚不清楚。修复材料含有氟化物、硅酸盐、钙、磷酸盐，有时也含有镁和银。我们在体外研究了不同离子对牙本质基质矿化的影响。脱矿牙本质基质在亚稳磷酸钙溶液中孵育，有或没有硅酸盐、氟化物、钙、磷酸盐、镁或银。在不添加离子的亚稳溶液中，可溶牙本质基质诱导矿物形成。硅酸盐和氟化物显著降低了不溶性牙本质基质对矿物质的诱导作用。至少需要钙或磷酸盐来促进矿物质诱导。相反，镁和银抑制矿物质诱导。每个样品孵育24h后诱导的矿物经x射线衍射图鉴定为磷灰石。结果表明，硅酸盐对牙本质基质矿化的诱导作用强于氟化物、钙和磷酸盐，而镁和银对牙本质基质矿化的诱导作用有抑制作用。

The effect of ions that could be released from restorative materials on the mineralization of dentin are not known. Restorative material contains fluoride, silicate, calcium, phosphate, and sometimes magnesium, and silver at well. We examined the effects of various ions on mineralization of dentin matrix in vitro. Demineralized dentin matrix was incubated in metastable calsium phosphate solution with or without silicate, fluoride, calcium, phosphate, magnesium or silver. In soluble dentin matrix induced mineral fomation in metastable solution without added ions. Silicate and fluoride significantly reduced mineral induction by insoluble dentin matrix. At least calcium or phosphate was required to promote mineral induction. Conversely, magnesium and silver inhibited mineral induction. The mineral induced by each sample after incubation for 24h was identified by its X-ray diffraction pattern as apatite. We conclude that silicate is a stronger inducer of remineralization of dentin matrix than fluoride, calcium or phosphate, and that magnesium and silver inhibit the induction of mineralization of dentin matrix.