Development of chair-side assay system for ongoing bone loss with peri-implant sulcus fluids

本研究的第一个目的是使用蛋白质印迹法将PISF样品中的MMP-1、MMP-8和MMP-13与牙周治疗相关联，并通过免疫组织化学法确定MMP-8的表达。从1990年至2000年期间在冈山大学医院就诊的64名连续患者中选择4名种植体周围炎患者和4名健康受试者（对照组）。仅在3例患者中观察到初始阳性试验。第一次采样后，2名患者接受了种植体周围机械和抗菌治疗，而1名患者由于无法定期到医院就诊而未接受积极治疗。在第二次PISF采样分析中，仅在一名未接受积极牙周治疗的患者中观察到对应于pro-MMP-8的85 kDa条带。在第二次PISF采样后半年，由于种植体周围炎的严重性，该患者的种植体不幸丢失。活动性种植体周围炎性结缔组织细胞周围可见MMP-8样免疫反应阳性。本研究的下一个目的是通过多变量分析确定获得和维持骨整合的危险因素。研究对象为1990年2月至2002年3月在我院安装并完成二次手术骨结合牙种植体的连续患者。基于调整后的多变量模型，烟草使用、颌骨和种植体涂层与骨整合的获得在统计学上相关。而影响骨结合的主要因素是颌骨和种植体长度。

The first aim of this study was to correlate MMP-1,MMP-8 and MMP-13 in the PISF samples collected from ongoing bone loss sites around per-implants with the periodontal treatments using Western blotting and to determine expression of MMP-8 by immunohistochemistry. Four peri-implantitis patients and four healthy subjects (control group) were selected from a sample of 64 consecutive patients who had attended the Okayama University Hospital from 1990 to 2000. Initial positive test was observed only in three patients. Following the first sampling, two patients received peri-implant mechanical and anti-microbial treatment, while one patient received no active treatment because of his inability to attend the hospital regularly. At the second PISF sampling analysis, an 85 kDa band corresponding to pro-MMP-8 was observed only in one patient received no active periodontal treatment. Half a year following the second PISF sampling, the implant in this patient unfortunately was lost because of severity of peri-implantitis. And MMP-8-like immunoreactivity was clearly seen around inflammatory connective tissue cells in from active peri-implantitis patients.The next aim of this study was to identify the risk factors in acquisition and maintenance of osseointegration using multivariate analysis. Subjects were consecutive patients who had been installed and finished the secondary surgery osseointegrated dental implants in our hospital from February 1990 to March 2002. Based on the adjusted multivariate models, tobacco use, jaw and implant coating were statistically associated with acquisition of osseointegration. While the risk factors in maintenance of osseointegration were jaw and implant length.