Risk assessment of peri-inplantitis patients with chair-side MMP-8 assay system

基质金属蛋白酶(MMPs)在牙周组织的病理组织破坏中起重要作用。因此，与骨基质降解相关的MMPs可能是种植体周围骨破坏的一个可能标记物。本研究从1990年至2000年在我校安装种植体支持的连续病例中选取4名种植体周围炎患者(男/女：2/2；平均年龄：68.5±6.7岁；9枚种植体)和4名年龄和性别匹配的对照组患者(男/女：2/2，平均年龄：66.0±5.8岁，8枚种植体)作为对照。本研究中选择种植体周围炎和对照患者的纳入标准分别为种植体年累积垂直骨丢失(ABL)大于0.6 mm和种植体年累积垂直骨丢失(ABL)小于0.2 mm。种植体周围骨水平通过每年拍摄的牙科x光片和平均值…进行评估使用三个校准的观察者读数中的更多的ES作为结果。还计算了上层建筑安装后每年持续的垂直骨丢失(OBL)。用吸湿纸尖收集牙周裂隙近中颊角处的牙周液。然后将样品溶解在含吐温20的0.1%磷酸盐缓冲液中(PBS-T)，并在-20℃下保存。用抗MMP1、MMP8和MMP13的抗人单抗(日本富士化学工业)，用免疫印迹法检测GCFs中的MMPs，并设阳性对照。结果显示，在种植体周围炎患者中，3颗牙周膜厚度大于0.8 mm的活动性骨吸收种植体中检测到了基质金属蛋白酶-8的表达，而在牙周炎患者的牙周组织中未检测到基质金属蛋白酶-8的表达。本研究未见MMP1和MMP13在GCFs中表达。这些结果表明，基质金属蛋白酶-8可能是预测种植体周围骨破坏的一个标记物。较少

Matrix metalloproteinases (MMPs) are known to play an important role in pathological tissue destruction in the periodontal tissues. Therefore, MMPs related to bone matrix degradation could be a possible marker for ongoing bone destruction around the peri-implantitis implants. In this study, four peri-implantitis patients (male/female : 2/2 ; mean age : 68.5^^+__-6.7 yrs ; 9 implants ) and age- and sex- matched four control patients (male/female : 2/2, mean age : 66.0^^+__-5.8 yrs, 8 implants) were selected from 64 consecutive patients who were installed implant-supported superstructures in our school from 1990 to 2000. Inclusion criteria for selection of the peri-implantitis and control patients in this study were having an implant "with accumulated vertical bone loss (ABL) of more than 0.6 mm per year" and "with ABL of less than 0.2 mm per year" after superstructure installation, respectively. Bone levels around implants were assessed by dental x-ray films taken annually and mean valu … More es of three calibrated observers readings were used as outcome. Ongoing vertical bone loss per year (OBL) after superstructure installation was also calculated. Gingival crevicular fluids (GCFs) were collected at the mesio-buccal comer of the gingival crevices by using absorbent paper points. Then the samples were dissolved in 0.1% phoshate buffered saline with Tween 20 (PBS-T) and stored at -20℃. MMPs in GCFs were detected by Western blotting using anti-human monoclonal antibodies specific for MMP-1, -8 and -13 (Fuji Chemical Industry, Japan) with positive controls. As the results, in the peri-implantitis patients, MMP-8 was detected in three actively bone-resorbing implants whose OBL at GCF sampling was more than 0.8 mm and not detected in the other six implants whose OBL at GCF sampling was less than 0.3 mm, while no MMP-8 was detected in the controls. MMP-1 and -13 could not be detected in any GCFs in this study. These results suggest that MMP-8 might be a marker for prediction of ongoing bone destruction around the peri-implantitis implants. Less