Risk assessment of peri-inplantitis patients with chair-side MMP-8 assay system

椅旁MMP-8检测系统对种植体周围炎患者的风险评估

• 批准号: 13672031
• 负责人: ARAKAWA Hikaru
• 金额: $ 2.56万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672031/
• 关键词: Dental Implants; Peri-implantitis; Matrix metalloproteinases; Gingival crevicular fluids; bone-resorption; Western blotting; Risk marker; MMPs; ELISA法; PCR法; コラゲナーゼ

Matrix metalloproteinases (MMPs) are known to play an important role in pathological tissue destruction in the periodontal tissues. Therefore, MMPs related to bone matrix degradation could be a possible marker for ongoing bone destruction around the peri-implantitis implants. In this study, four peri-implantitis patients (male/female : 2/2 ; mean age : 68.5^^+__-6.7 yrs ; 9 implants ) and age- and sex- matched four control patients (male/female : 2/2, mean age : 66.0^^+__-5.8 yrs, 8 implants) were selected from 64 consecutive patients who were installed implant-supported superstructures in our school from 1990 to 2000. Inclusion criteria for selection of the peri-implantitis and control patients in this study were having an implant "with accumulated vertical bone loss (ABL) of more than 0.6 mm per year" and "with ABL of less than 0.2 mm per year" after superstructure installation, respectively. Bone levels around implants were assessed by dental x-ray films taken annually and mean valu … More es of three calibrated observers readings were used as outcome. Ongoing vertical bone loss per year (OBL) after superstructure installation was also calculated. Gingival crevicular fluids (GCFs) were collected at the mesio-buccal comer of the gingival crevices by using absorbent paper points. Then the samples were dissolved in 0.1% phoshate buffered saline with Tween 20 (PBS-T) and stored at -20℃. MMPs in GCFs were detected by Western blotting using anti-human monoclonal antibodies specific for MMP-1, -8 and -13 (Fuji Chemical Industry, Japan) with positive controls. As the results, in the peri-implantitis patients, MMP-8 was detected in three actively bone-resorbing implants whose OBL at GCF sampling was more than 0.8 mm and not detected in the other six implants whose OBL at GCF sampling was less than 0.3 mm, while no MMP-8 was detected in the controls. MMP-1 and -13 could not be detected in any GCFs in this study. These results suggest that MMP-8 might be a marker for prediction of ongoing bone destruction around the peri-implantitis implants. Less

基质金属蛋白酶（MMPs）在牙周组织的病理组织破坏中起重要作用。因此，与骨基质降解相关的MMPs可能是种植体周围正在进行的骨破坏的可能标志。本研究从我院1990 - 2000年连续64例种植体支撑上层建筑患者中选择4例种植体周围炎患者（男/女2/2，平均年龄68.5^^+_ -6.7岁，种植体9个）和4例年龄性别匹配的对照患者（男/女2/2，平均年龄66.0^^+_ -5.8岁，种植体8个）。本研究中选择种植体周围炎患者和对照患者的入选标准分别是在上层建筑安装后种植体“累积垂直骨丢失（ABL）每年大于0.6 mm”和“ABL每年小于0.2 mm”。通过每年拍摄的牙科x光片和平均值来评估种植体周围的骨水平。同时计算上层建筑安装后每年持续的垂直骨损失（OBL）。采用吸湿纸点在龈缝中颊角处收集龈沟液。将样品溶解于含Tween 20 （PBS-T）的0.1%磷酸盐缓冲盐水中，-20℃保存。采用MMP-1、-8和-13特异性抗人单克隆抗体（Fuji Chemical Industry, Japan），以阳性对照检测gcf中的MMPs。结果显示，在种植体周围炎患者中，3个GCF取样OBL大于0.8 mm的主动骨吸收种植体中检测到MMP-8，其余6个GCF取样OBL小于0.3 mm的种植体中未检测到MMP-8，而对照组中未检测到MMP-8。MMP-1和-13在本研究中未检测到。这些结果表明MMP-8可能是预测种植体周围正在进行的骨破坏的标记物。少

项目成果

荒川 光 ほか: "口腔インプラント患者の分子生物学的マーカによるリスク評価-口腔インプラント周囲歯肉溝滲出液中のMMPsの検出-"日本口腔インプラント学会誌. 14・1. 99 (2001)

Hikaru Arakawa 等人：“使用分子生物学标记对口腔种植患者进行风险评估 - 口腔种植体周围龈沟液中 MMP 的检测 -”日本口腔种植学会杂志 14, 1. 99 (2001)。

荒川 光ほか: "オッセオインテグレーションの獲得と維持からみた口腔インプラントの臨床予後評価"日本補綴歯科学会雑誌. 46特別号. 58 (2002)

Hikaru Arakawa 等人：“从骨整合的获得和维持角度评估口腔种植体的临床预后”，日本修复医学会杂志 46 号特刊（2002 年）。

H. Arakawa: "Oral implant treatment after extracing replanted teeth with severe root and bone resorption : A case report"J Jpn Prosthodont Soc. 46, (in Japanese). 758-759 (2002)

H. Arakawa：“拔除重植牙后的口腔种植治疗，伴有严重的牙根和骨吸收：病例报告”Jpn Prosthodont Soc。

H. Arakawa et al: "Risk assessment of peri-inplantitis patients with Matrix metalloproteiases in gingival crevicular fluids"J.Jpn.Soc.Oral Implant. 14, (in Japanese). 99 (2001)

H. Arakawa 等人：“龈沟液中基质金属蛋白酶对种植体周围炎患者的风险评估”J.Jpn.Soc.Oral Implant。

荒川 光 ほか: "口腔インプラントの生存に関する疫学調査:オッセオインテグレーションの獲得と維持からみた評価"日本口腔インプラント学会誌. 15・1. 67-74 (2002)

Hikaru Arakawa 等：“口腔种植体存活的流行病学调查：从骨整合的获得和维持的角度进行评估”日本口腔种植学会杂志 15・1（2002 年）。