Development of new prevention method for stomatitis caused by Candida induced by the use of denture in elderlies

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To examine the possibility of a vaccine for candidiasis, we induced salivary antibodies to Candida albicans by weekly-applications of killed-C.albicans onto palatine tonsils of rabbits for six weeks. Significant levels of antibodies to C.albicans were seen in saliva and blood plasma by direct agglutination and ELISA. The saliva after immunization greatly inhibited C.albicans adherence to cloned human epithelial cells, while the saliva before immunization had no effect on the adherence. From the results of selective inhibition in ELISA reaction and agglutination by commercial antibodies to C.albicans, around 80% of the salivary antibodies were shown to react with one of the mannoprotein antigens, called factor 6 which has been shown to be involved of C.albicans adherence to epithelial cells. Therefore, tonsillar applications of C.albicans induce salivary antibodies that prevent C.albicans adherence to epithelial cells, and thus should prove useful in prevention of oral candidiasis.