Development of new prevention method for stomatitis caused by Candida induced by the use of denture in elderlies

老年人戴假牙诱发念珠菌性口腔炎预防新方法的研制

• 批准号: 15592059
• 负责人: NAGAMATSU Hiroshi
• 金额: $ 2.18万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592059/
• 关键词: mucosal immunity; Candida albicans; palatine tonsil; immunization; 唾液; IgA; カンジダ; 義歯性口内炎; ワクチン

To examine the possibility of a vaccine for candidiasis, we induced salivary antibodies to Candida albicans by weekly-applications of killed-C.albicans onto palatine tonsils of rabbits for six weeks. Significant levels of antibodies to C.albicans were seen in saliva and blood plasma by direct agglutination and ELISA. The saliva after immunization greatly inhibited C.albicans adherence to cloned human epithelial cells, while the saliva before immunization had no effect on the adherence. From the results of selective inhibition in ELISA reaction and agglutination by commercial antibodies to C.albicans, around 80% of the salivary antibodies were shown to react with one of the mannoprotein antigens, called factor 6 which has been shown to be involved of C.albicans adherence to epithelial cells. Therefore, tonsillar applications of C.albicans induce salivary antibodies that prevent C.albicans adherence to epithelial cells, and thus should prove useful in prevention of oral candidiasis.

为了研究念珠菌病疫苗的可能性，我们通过每周一次将杀死的白色念珠菌应用于兔子的腭扁桃体上六周来诱导唾液中的白色念珠菌抗体。通过直接凝集和ELISA，在唾液和血浆中观察到显著水平的抗白色念珠菌抗体。免疫后的唾液对白念珠菌在克隆人上皮细胞上的粘附有明显的抑制作用，而免疫前的唾液对白念珠菌在克隆人上皮细胞上的粘附无影响。从ELISA反应中的选择性抑制和商业抗体对白色念珠菌的凝集的结果来看，约80%的唾液抗体显示与甘露糖蛋白抗原之一反应，称为因子6，其已显示参与白色念珠菌对上皮细胞的粘附。因此，扁桃体应用白色念珠菌诱导唾液抗体，防止白色念珠菌粘附上皮细胞，因此应该证明在预防口腔念珠菌病有用。