Effects of Platelet Rich Plasma(PRP) for the Patients with Cleft of Cleft Lip and Palate

富血小板血浆（PRP）对唇腭裂患者的疗效

• 批准号: 15592120
• 负责人: MORIYMA Taiji
• 金额: $ 1.79万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592120/
• 关键词: Platelet rich plasma; cleft lip and palate; TGF-β1; Aluminum equivalence value; 唇顎口蓋裂; TGF-β2; アルミ当量値; 多血小板血漿(PRP); TFG-β2; 骨移植

The postoperative changes in the grafted bone were evaluated in 28 patients with cleft lip and palate (34 clefts) who had undergone iliac bone grafting in the alveolar cleft using platelet rich plasma(PRP)(PRP group), and compared to those in 17 patients with cleft lip and palate(17 clefts) who had undergone grafting without PRP(non-PRP group).Dental X-ray with an aluminum wedge was taken in the grafted bone in the alveolar cleft, 1 week, and 1,3, and 6 months postoperatively, and the levels of the upper and lower margins of the bone bridge in the alveolar cleft was measured. The bone density on the dental X-ray film was also measured on an aluminum-equivalence(Al-Eq) value based on aluminum wedge with a thickness of 0 to 16 mm was mounted on the film holder. The surgical outcome was regarded as good when the level of the upper margin of the bone bridge was 1.0 or less and that of the lower margin was 3.0 or more 6 months postoperatively and as failure otherwise, and was evaluated on b … More oth groups.The postoperative change of the bone grafting by iliac block bone and the distraction osteogenesis using PRP in alveolar cleft was evaluated in a patient with unilateral cleft lip and palate.The results were as follows :1.Preoperatively, the mean number of platelets in the plasma was 27.7×10^4/μl, and that in the PRP was 86.8×10^4/μl, showing a concentration rate of 319.9%. The mean amount of TGF-β1 in the plasma was 4.4 ng/ml, and that in the PRP was 191.7 ng/ml, which was 51.5-fold higher than in the former. It was confirmed that the number of platelets and the amount of TGF-β1 in the PRP were increased.2.The Al-Eq rapidly decreased up to 3 months postoperatively in the PRP group, and slightly increased 6 months postoperatively. In the non-PRP group, the Al-Eq gradually decreased up to 6 months postoperatively. Bone regeneration was slightly more rapid in the PRP group than in the non-PRP group.3.The lower margin of the bone bridge was slightly reduced between 1 week and 6 months postoperatively in the PRP and non-PRP groups, but the differences were not significant. The upper margin of the bone bridge was rapidly absorbed up to 1 month postoperatively in both groups, and then remained almost unchanged. Good outcomes 6 months postoperatively were observed in 12 of the 17 patients in the PRP group (70.6%), and in 9 of the 17 patients in the non-PRP group (52.9%), the difference being significant.4.The rate of change in the bone bridge width between 1 week and 6 months postoperatively was 77.2% in the PRP group and 71.7% in the non-PRP group, indicating that bone absorption was slightly lower in the former than in the latter.5.In grafted bone by iliac block bone, the Al-Eq rapidly decreased up to 3 months postoperatively, and the rate of change in Al-Eq value width between 1 week and 1 year postoperatively was 90.7%.These findings suggested that PRP enhanced bone regeneration by acting on the autogenous iliac bone, resulting in good outcomes of iliac bone grafting. Less

对28例唇腭裂患者术后移植骨的变化进行了评价（34例）在牙槽突裂中使用富血小板血浆（PRP）进行髂骨移植（PRP组），并与17例唇腭裂患者进行比较（17例裂）在没有PRP的情况下接受移植术后1周、1个月、3个月和6个月，测量牙槽突裂骨桥上、下缘的水平。还根据铝当量（Al-Eq）值测量牙科X射线胶片上的骨密度，该值基于安装在胶片保持器上的厚度为0至16 mm的铝楔。术后6个月，骨桥上缘水平≤ 1.0，下缘水平≥ 3.0为良好，否则为失败，按B分级 ...更多信息 对1例单侧唇腭裂患者行髂骨块植骨和PRP牵张成骨术后的变化进行了观察，结果如下：1.术前血浆血小板平均数为27.7× 104/μl，PRP平均数为86.8× 104/μ l，血小板浓缩率为319.9%。血浆中TGF-β1的平均含量为4.4ng/ml，PRP中TGF-β1的平均含量为191.7ng/ml，是前者的51.5倍。PRP组术后3个月Al-Eq迅速下降，6个月略有上升。在非PRP组中，Al-Eq在术后6个月逐渐降低。PRP组骨再生速度略快于非PRP组。3. PRP组和非PRP组骨桥下缘在术后1周和6个月间略有缩小，但差异无统计学意义。两组骨桥上缘在术后1个月内迅速吸收，然后几乎保持不变。PRP组17例患者中有12例在术后6个月观察到良好结局（70.6%），非PRP组17例患者中有9例术后1周至6个月骨桥宽度变化率PRP组为77.2%，非PRP组为71.7%，5.髂骨块移植骨的Al-Eq值在术后3个月内迅速下降，术后1周至1年的Al-Eq值宽度变化率为90.7%，提示PRP通过作用于自体髂骨促进骨再生，导致髂骨移植的良好结果。少