Histopathological analysis for the effects of shear force caused by position change on pressure ulcer healing

体位改变剪切力对压疮愈合影响的组织病理学分析

• 批准号: 15592226
• 负责人: SUGAMA Junko
• 金额: $ 1.92万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592226/
• 关键词: pressure ulcer; animal model; shear force; wound healing; histopathology; ラットモデル

Shear force injury occurs when the skin remains stationary and the underlying tissue shifts. This shift diminishes blood supply to the skin and soon results in ischemia and tissue damage. However, no satisfactory animal models of a shear force induced ischemic wound have been constructed, and the effects of shear force on the healing process of such wounds have yet to be clarified. The present study therefore aimed to prepare a shear force induced ischemic wound model and histologically clarify the effects of shear force on wound healing.Two incisions were made down to the peritoneal cavity in the flank region of 16 rats, and a metal plate was passed through one incision under the peritoneum and then exited from the other. From the exterior surface, 8 kg of pressure was applied for 6 hours to the flank skin and abdominal muscle to prepare an ischemic wound. The wound was observed for two weeks, and histological tissue sections were prepared on days 7 and 14. Wound healing was compared between two groups : a shear force group (1.0 kg shear force loading) and a control group (only pressure was applied to prepare an'ischemic wound).It took one week longer for a shear force induced ischemic wound to heal than a pressure induced ischemic wound. Histopathlogical findings of the shear force induced ischemic wound were the caudal muscle layer's degeneration and the cranial dermis layer's congestion and hemorrhage. Control group's histopathlogical findings were muscle layer's degeneration on Day 7, and tissue regeneration on Day 14.

当皮肤保持静止而下面的组织移位时，发生剪切力损伤。这种转变减少了皮肤的血液供应，很快导致局部缺血和组织损伤。然而，还没有建立满意的剪切力诱导的缺血性伤口的动物模型，并且剪切力对这种伤口的愈合过程的影响还有待澄清。因此，本研究旨在制备剪切力诱导的缺血性伤口模型，并从组织学上阐明剪切力对伤口愈合的影响。在16只大鼠的侧腹区域向下做两个切口至腹膜腔，将金属板穿过腹膜下的一个切口，然后从另一个切口退出。从外表面对侧腹皮肤和腹部肌肉施加8 kg压力6小时以制备缺血性伤口。观察伤口两周，并在第7天和第14天制备组织学组织切片。比较剪切力组（1.0kg剪切力负荷）和对照组（仅施加压力以制备“缺血性伤口”）的伤口愈合情况，剪切力诱导的缺血性伤口比压力诱导的缺血性伤口愈合时间长一周。剪切力致缺血性损伤的组织学表现为尾侧肌层变性和头侧真皮层充血出血。对照组的组织病理学结果为第7天的肌肉层变性和第14天的组织再生。