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Detection and quantification of aldehyde dehydrogenase activities in Japanese young female students using genetic and psychiatrist tests of the alcoholism

使用遗传和精神病学家的酒精中毒测试来检测和定量日本年轻女学生的乙醛脱氢酶活性

基本信息

批准号：
16500144
负责人：
TAKII Yukio
金额：
$ 2.3万
依托单位：
Mukogawa Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Genetic and epidemiological studies on alcohol tolerance frequencies were conducted by analyzing genetic materials from 149 healthy female students. Aldehyde dehydrogenase 2 (ALDH2) gene was characterized by combination of polymerase chain reaction and agarose gel electrophoresis of respective DNA specimen.Nail clippings were found to be relatively easy to collect and extract from volunteers especially when they restricted to female students. Plucking hair roots was not successful, since it caused sometimes pains on the volunteers. Among 149 individuals tested, 65.6% were the typical or normal homozygote, 32.2% were the heterozygote, and 2.0% were the atypical mutant homozygote.The reliability of the scale ratings using ALDH2 genotype analysis was assessed and confirmed by ethanol patch tests and other psychiatrists evaluation methods such as TAST (Tokyo University ALDH2 Phenotype Screening Test). However, the genotype analysis is not directly associated with AAIS (Adolescent Alcohol Involvement Scale). Accordingly, careful case-control study must be undertaken on genotype analysis and it must be accompanied by several combinations of epidemiological studies.

对149名健康女大学生的遗传资料进行了酒精耐受率的遗传学和流行病学研究。用聚合酶链式反应和琼脂糖凝胶电泳法相结合的方法对乙醛脱氢酶2(ALDH2)基因进行了分析。研究发现，从志愿者身上采集和提取指甲切片相对容易，尤其是当指甲切片仅限于女生时。拔掉发根并不成功，因为这有时会给志愿者带来疼痛。在149名受试者中，65.6%为典型或正常纯合子，32.2%为杂合子，2.0%为非典型突变纯合子。通过酒精斑贴试验和其他精神科医生评估方法，如东京大学ALDH2表型筛选试验，对ALDH2基因分析量表评分的可靠性进行了评估和确认。然而，基因分析与青少年酗酒量表(AAIS)并不直接相关。因此，必须对基因分析进行仔细的病例对照研究，并且必须伴随着几种流行病学研究的组合。

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

液体培養系におけるグルコアミラーゼ高生産麹菌株の取得

在液体培养系统中获得高产葡糖淀粉酶的曲霉菌株

DOI：
发表时间：
2006
期刊：
温古知新 43
影响因子：
0
作者：
T.;Miura;T.;Tanaka;Y.;Suemitsu;Shigetoshi;Nara;瀧井幸男
通讯作者：
瀧井幸男

Purification and characterization of β-glucosidase from Rhizopus oryzae MIBA348

米根霉 MIBA348 β-葡萄糖苷酶的纯化和表征

DOI：
发表时间：
2005
期刊：
Journal of Biological Macromolecule 5
影响因子：
0
作者：
Yukio Takii;Kimiko Ikeda;Chihiro Sato;Megumu Yano;Tsutomu Sato;Hiroshi Konno
通讯作者：
Hiroshi Konno

麹菌(Aspergillus oruzae)グルコアミラーゼ高生産株の取得と酵素学的特性

米曲霉糖化酶高产菌株的获得及酶学性质