Functional analysis of Galanin-like peptide (GALP) in feeding regulation

甘丙肽样肽（GALP）在摄食调节中的功能分析

• 批准号: 16500228
• 负责人: GUAN Jian-Lian
• 金额: $ 2.24万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500228/
• 关键词: Galanin-like peptide; GPCR; feeding regulation; hypothalamus; 神経ペプチド; ニューロンネットワーク; シナプス; 免疫電顕

The hypothalamus plays a critical role in regulation of feeding behavior, energy metabolism and reproduction. A novel peptide discovered in porcine hypothalamus has 60 amino acid peptide and a non-amidated C-terminus. Since a portion of this peptide is homology with galanin, it is named galanin-like peptide (GALP). GALP is mainly produced in the hypothalamic arcuate nucleus. GALP acts in the central nervous system, where it is involved in the regulation of feeding behavior. GALP-producing neurons make neuron networks with several feeding related peptide- producing neurons. Since GALP controls food intake and energy balance, it is possible that GALP is an important factor in development of obesity. Furthermore, we have succeeded to make transgenic (Tg) mice that express green fluorescence (EGFP) in GALP-containing neurons and also to make Tg mice that express EGFP in afferent GALP neurons in brain. As a result, it appeares that GALP-containing neurons make synaptic input from the piriform cortex and amygdala. Therefore, GALP may involve in feeding behavior, energy metabolism and reproduction, further studies on the morphology and function of GALP should continue to demonstrate further the understanding in brain function.

下丘脑在摄食行为、能量代谢和生殖调控中起着重要作用。在猪下丘脑中发现的一种新肽具有60个氨基酸的肽和非酰胺化的C-末端。由于该肽的一部分与甘丙肽同源，因此将其命名为甘丙肽样肽（Galanin-like peptide，GALP）。GALP主要产生于下丘脑弓状核。GALP作用于中枢神经系统，参与摄食行为的调节。产生GALP的神经元与几个与进食相关的产生肽的神经元形成神经元网络。由于GALP控制食物摄入和能量平衡，因此GALP可能是肥胖发展的重要因素。此外，我们已经成功地制造了在含GALP的神经元中表达绿色荧光（EGFP）的转基因（Tg）小鼠，并且还制造了在脑中的传入GALP神经元中表达EGFP的Tg小鼠。结果表明，含GALP的神经元从梨状皮质和杏仁核进行突触输入。因此，GALP可能参与摄食行为、能量代谢和生殖，对其形态学和功能的进一步研究将有助于进一步了解其脑功能。

项目成果

Neuronal protein kinase Cγ-dependent proliferation/hypertrophy of spinal cord astrocyte following repeated in vivo administration of morphine.

重复体内给予吗啡后脊髓星形胶质细胞的神经元蛋白激酶 Cγ 依赖性增殖/肥大。

• 发表时间: 2004
• 期刊: Eur.J.Neurosci. 19
• 作者: M.Narita;M.Suzuki;M.Narita;Y.Yajima;R.Suzuki;S.Shioda;T.Suzuki
• 通讯作者: T.Suzuki

Involvement of IL-6 and tumor necrosis factor alpha in CYP 3A11 and 2C29 down-regulation by Bacillus calmette-guerin and Lipopolysaccharide in mouse liver.

IL-6 和肿瘤坏死因子 α 参与卡介苗和脂多糖对小鼠肝脏中 CYP 3A11 和 2C29 的下调。

• 发表时间: 2004
• 期刊: Drug Metab.Dispos. 32
• 作者: Isogawa K;Akiyoshi J;Kodama K;Matsushita H;Tsutsumi T;Funakoshi H;Nakamura T;Ashino T.
• 通讯作者: Ashino T.

Electron microscopy examination of galanin-like peptide (GALP)-containing neurons in the rat hypothalamus

• DOI: 10.1016/j.regpep.2004.08.011
• 发表时间: 2005-03-15
• 期刊: REGULATORY PEPTIDES
• 作者: Guan, JL;Kageyama, H;Shioda, S
• 通讯作者: Shioda, S

Orexin-1 receptor expression after global ischemia in mice

• DOI: 10.1016/j.regpep.2004.08.037
• 发表时间: 2005-03-15
• 期刊: REGULATORY PEPTIDES
• 作者: Nakamachi, T;Endo, S;Shioda, SJ
• 通讯作者: Shioda, SJ

Mishandling of the therapeutic peptide glucagons generates cytotoxic amyloidogenic fibrils.

治疗性肽胰高血糖素处理不当会产生细胞毒性的淀粉样原纤维。

• 发表时间: 2004
• 期刊: Pharmaceut.Res. 21
• 作者: Nakajima;O.;Nakamura;F.;Yamashita;N.;Tomita;Y.;Syto;F.;Okada;T.;Iwamattsu;A.;Kondo;E.;Fujisawa;H.;Takei;K.;Goshima;Y.;Onoue S.
• 通讯作者: Onoue S.